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  • For rapid and simple simultaneous purification of all sizes of cell-free circulating DNA (cfc-DNA), and circulating tumour DNA (Ct-DNA) and circulating/exosomal RNA, including microRNA from the same plasma/serum sample
  • Versatile plasma/serum input ranging from 1 mL to 6 mL
  • No phenol extractions or carrier RNA 
  • Minimal high molecular weight gDNA contamination in the purified cfc-DNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL
  • Purify superior-quality and superior-quantity RNA in 45 minutes
  • Fully automated purification procedure on Hamilton MicroLab Nimbus
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

Related Services


ItemsCat. #Size
Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit 68300 50 preps

  • For rapid and simple simultaneous purification of all sizes of cell-free circulating DNA (cfc-DNA), and circulating tumour DNA (Ct-DNA) and circulating/exosomal RNA, including microRNA from the same plasma/serum sample
  • Versatile plasma/serum input ranging from 1 mL to 6 mL
  • No phenol extractions or carrier RNA 
  • Minimal high molecular weight gDNA contamination in the purified cfc-DNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL
  • Purify superior-quality and superior-quantity RNA in 45 minutes
  • Fully automated purification procedure on Hamilton MicroLab Nimbus
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

Related Services



Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit

Norgen’s cfc-DNA/cfc-RNA Advanced Fractionation Kit provides fast, reliable and simple procedures for the fractionation of the highest quality and quantity of all sizes of circulating DNA and circulating RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL into two separate elutions. Purification is based on using Norgen’s proprietary resin separation matrix. The kits are designed to isolate total cfc-nucleic acid from either fresh, preserved or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-RNA and cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum total cfc-nucleic acid is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications
Minimum Plasma/Serum Input
1 mL
Maximum Plasma/Serum Input
6 mL
Size of cfc-RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)
Optimal size of purified cfc-DNA
146 - 180 bp
Elution Volume
25-50 µL
Time to Complete 10 Purifications
45 minutes
Average Yields* Variable depending on specimen

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit contains ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.


Protocols: 
ItemsCat. #Size
Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit 68300 50 preps

  • For rapid and simple simultaneous purification of all sizes of cell-free circulating DNA (cfc-DNA), and circulating tumour DNA (Ct-DNA) and circulating/exosomal RNA, including microRNA from the same plasma/serum sample
  • Versatile plasma/serum input ranging from 1 mL to 6 mL
  • No phenol extractions or carrier RNA 
  • Minimal high molecular weight gDNA contamination in the purified cfc-DNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL
  • Purify superior-quality and superior-quantity RNA in 45 minutes
  • Fully automated purification procedure on Hamilton MicroLab Nimbus
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

Related Services



Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit

Norgen’s cfc-DNA/cfc-RNA Advanced Fractionation Kit provides fast, reliable and simple procedures for the fractionation of the highest quality and quantity of all sizes of circulating DNA and circulating RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL into two separate elutions. Purification is based on using Norgen’s proprietary resin separation matrix. The kits are designed to isolate total cfc-nucleic acid from either fresh, preserved or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-RNA and cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum total cfc-nucleic acid is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications
Minimum Plasma/Serum Input
1 mL
Maximum Plasma/Serum Input
6 mL
Size of cfc-RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)
Optimal size of purified cfc-DNA
146 - 180 bp
Elution Volume
25-50 µL
Time to Complete 10 Purifications
45 minutes
Average Yields* Variable depending on specimen

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit contains ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2.5 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Protocols: 
 

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