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For the rapid and simple isolation of circulating DNA from plasma/serum samples
For research use only and NOT intended for in vitro diagnostics.
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Kit Specifications
Kit Specifications
|
|
Minimum Plasma/Serum Input |
50 μL
|
Maximum Plasma/Serum Input |
400 μL
|
Minimum Elution Volume |
100 μL
|
Time to Complete Purifications |
< 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Kit Specifications
|
|
Minimum Plasma/Serum Input
|
400 μL
|
Maximum Plasma/Serum Input
|
2 mL
|
Minimum Elution Volume |
100 μL
|
Time to Complete Purifications
|
< 45 minutes
|
Storage Conditions and Product Stability
It is recommended to warm Slurry C2 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry C2 contains a grey resin that will not dissolve with warming.
Kit Specifications
|
|
Minimum Plasma/Serum Input
|
2 mL
|
Maximum Plasma/Serum Input
|
10 mL
|
Size of DNA Purified |
≥ 50 bp
|
Elution Volume |
100 μL
|
Time to Complete 10 Purifications |
15-20 minutes
|
Average Yields |
Variable depending on specimen
|
Note: Do not exceed the recommended sample input volume of 10 mL.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
Mini (50600)
Title | Comparison of the Effectiveness of Four Commercial DNA Extraction Kits on Fresh and Frozen Human Milk Samples |
Journal | Methods Protoc. 2022. |
Author | Cassidy Butler, Amy Matsumoto, Casey Rutherford and Hope K. Lima |
Title | Optimization of DNA Extraction from Human Milk |
Journal | Digital Commons @ Winthrop Universtity. Spring 5-2020. |
Author | Cassandra Z. Rutherford. |
Title | Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma |
Journal | Analytical and Bioanalytical Chemistry. 2015. |
Authors | Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J. |
Title | Cow’s Milk Contamination of Human Milk Purchased via the Internet |
Journal | Pediatrics. 2015. |
Authors | Sarah A. Keim, Manjusha M. Kulkarni, Kelly McNamara, Sheela R. Geraghty, Rachael M. Billock, Rachel Ronau, Joseph S. Hogan, Jesse J. Kwiek |
Title | Microbial contamination of human milk purchased via the Internet |
Journal | Pediatrics. 2013. |
Authors | Keim SA, Hogan JS, McNamara KA, Gudimetla V, Dillon CE, Kwiek JJ, Geraghty SR. |
Maxi (51300)
Title | Loa loa and Onchocerca ochengi miRNAs detected in host circulation |
Journal | Molecular and Biochemical Parasitology. 2014. |
Authors | Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary. |
Plasma/Serum Circulating DNA Purification Kits (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Minimum Plasma/Serum Input |
50 μL
|
Maximum Plasma/Serum Input |
400 μL
|
Minimum Elution Volume |
100 μL
|
Time to Complete Purifications |
< 30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Kit Specifications
|
|
Minimum Plasma/Serum Input
|
400 μL
|
Maximum Plasma/Serum Input
|
2 mL
|
Minimum Elution Volume |
100 μL
|
Time to Complete Purifications
|
< 45 minutes
|
Storage Conditions and Product Stability
It is recommended to warm Slurry C2 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry C2 contains a grey resin that will not dissolve with warming.
Kit Specifications
|
|
Minimum Plasma/Serum Input
|
2 mL
|
Maximum Plasma/Serum Input
|
10 mL
|
Size of DNA Purified |
≥ 50 bp
|
Elution Volume |
100 μL
|
Time to Complete 10 Purifications |
15-20 minutes
|
Average Yields |
Variable depending on specimen
|
Note: Do not exceed the recommended sample input volume of 10 mL.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Mini (50600)
Title | Comparison of the Effectiveness of Four Commercial DNA Extraction Kits on Fresh and Frozen Human Milk Samples |
Journal | Methods Protoc. 2022. |
Author | Cassidy Butler, Amy Matsumoto, Casey Rutherford and Hope K. Lima |
Title | Optimization of DNA Extraction from Human Milk |
Journal | Digital Commons @ Winthrop Universtity. Spring 5-2020. |
Author | Cassandra Z. Rutherford. |
Title | Comprehensive evaluation of methods to isolate, quantify, and characterize circulating cell-free DNA from small volumes of plasma |
Journal | Analytical and Bioanalytical Chemistry. 2015. |
Authors | Mauger F, Dulary C, Daviaud C, Deleuze JF, Tost J. |
Title | Cow’s Milk Contamination of Human Milk Purchased via the Internet |
Journal | Pediatrics. 2015. |
Authors | Sarah A. Keim, Manjusha M. Kulkarni, Kelly McNamara, Sheela R. Geraghty, Rachael M. Billock, Rachel Ronau, Joseph S. Hogan, Jesse J. Kwiek |
Title | Microbial contamination of human milk purchased via the Internet |
Journal | Pediatrics. 2013. |
Authors | Keim SA, Hogan JS, McNamara KA, Gudimetla V, Dillon CE, Kwiek JJ, Geraghty SR. |
Maxi (51300)
Title | Loa loa and Onchocerca ochengi miRNAs detected in host circulation |
Journal | Molecular and Biochemical Parasitology. 2014. |
Authors | Lucienne Tritten, Maeghan O’Neill, Samuel Wanji, Abdel Njouendoui, Fanny Fombad, Jonas Kengne-Ouaffo, Charles Mackenzie, Timothy Geary. |