Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Cat. 51200
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Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Cat. 51200

For the rapid and simple isolation of circulating DNA from plasma/serum samples

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Versatile plasma and serum input volumes (0.4 mL to 2 mL)
  • Concentrate circulating DNA into small elution volumes
  • Isolate inhibitor-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
  • Purification is based on Norgen’s proprietary resin matrix

For research use only and NOT intended for in vitro diagnostics.

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Plasma/Serum Circulating DNA Isolation Midi Kit (Slurry Format)
(Cat. 51200)
20 Preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Versatile plasma and serum input volumes (0.4 mL to 2 mL)
  • Concentrate circulating DNA into small elution volumes
  • Isolate inhibitor-free circulating DNA
  • Compatible with Streck Cell-Free DNA BCT® Tubes
  • Purification is based on Norgen’s proprietary resin matrix

Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides an efficient method for the purification of fragmented free-circulating DNA from 0.4 mL to 2 mL of human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit.

Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100 ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. Preparation time for a single sample is less than 45 minutes. The purified plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

Kit Specifications
Minimum Plasma/Serum Input
400 μL
Maximum Plasma/Serum Input
2 mL
Minimum Elution Volume
100 μL
Time to Complete Purifications
< 45 minutes


Storage Conditions and Product Stability
It is recommended to warm Slurry C2 and Lysis Buffer A for 20 minutes at 60oC if any salt precipitation is observed. Slurry C2 contains a grey resin that will not dissolve with warming.


Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides an efficient method for the purification of fragmented free-circulating DNA from 0.4 mL to 2 mL of human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit.

Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100 ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. Preparation time for a single sample is less than 45 minutes. The purified plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

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You have selected: Cat. 51200
Kit Specifications
Minimum Plasma/Serum Input
400 μL
Maximum Plasma/Serum Input
2 mL
Minimum Elution Volume
100 μL
Time to Complete Purifications
< 45 minutes


Storage Conditions and Product Stability
It is recommended to warm Slurry C2 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry C2 contains a grey resin that will not dissolve with warming.

 


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