Pospiviroid TaqMan RT-PCR Detection Kits Powered by Bioz Pospiviroid TaqMan RT-PCR Detection Kits SKU TM53750 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the Pospiviroid Available for TaqMan format for analysis Pospiviroid belongs to the Pospiviroidae family on the basis of biochemical, biological and structural properties which have been classified. The genus Pospiviroid contains nine viroid species: Potato spindle tuber viroid (PSTVd), Tomato chlorotic dwarf viroid (TCDVd), Mexican papito viroid (MPVd), Tomato planta macho viroid (TPMVd), Chrysanthemum stunt viroid (CSVd), Citrus exocortis viroid (CEVd), Tomato apical stunt viroid (TASVd), Iresine viroid (IrVd) and Columnea latent viroid (CLVd). Viroids cannot be detected by immunological methods because they do not encode any proteins, therefore an accurate and reliable diagnostic method for the detection of viroids, followed by eradication of the infected sources, is needed for quarantine operations. Norgen’s Pospiviroid Viroid RT-PCR Detection Kit allows for the detection of any of the 9 viroid species of the Pospiviroid family. TaqMan PCR Kit Click to expand options Pospiviroid TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Pospiviroid TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM53750 (100 preps) Cat. TM53710 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Pospiviroid Primer & Probe Mix 280 μL 280 μL Pospiviroid Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS PITM53750-1 Pospiviroid TaqMan RT-PCR Kit Insert.pdf (TM53710) Pospiviroid TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Plant/Fungi Total RNA Purification Kit
Pospiviroid TaqMan RT-PCR Detection Kits Powered by Bioz Pospiviroid TaqMan RT-PCR Detection Kits SKU TM53750 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
