Pospiviroid belongs to the Pospiviroidae family on the basis of biochemical, biological and structural properties which have been classified. The genus Pospiviroid contains nine viroid species: Potato spindle tuber viroid (PSTVd), Tomato chlorotic dwarf viroid (TCDVd), Mexican papito viroid (MPVd), Tomato planta macho viroid (TPMVd), Chrysanthemum stunt viroid (CSVd), Citrus exocortis viroid (CEVd), Tomato apical stunt viroid (TASVd), Iresine viroid (IrVd) and Columnea latent viroid (CLVd). Viroids cannot be detected by immunological methods because they do not encode any proteins, therefore an accurate and reliable diagnostic method for the detection of viroids, followed by eradication of the infected sources, is needed for quarantine operations. Norgen’s Pospiviroid Viroid RT-PCR Detection Kit allows for the detection of any of the 9 viroid species of the Pospiviroid family.
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Pospiviroid TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Pospiviroid TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.
TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
