Plum Pox Virus (PPV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plum Pox Virus (PPV) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM33450 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for PPV Available in TaqMan format for analysis Plum pox virus (PPV) is a member of the genus Potyvirus, and the virus is considered the most serious pathogen affecting stone fruits including apricots, cherry, nectarine, peaches, and plums. The symptoms of plum pox disease or Sharka include fruit abnormality, loss of taste, and fruits that drop prematurely. These symptoms may cause losses as high as 80–100% of a crop and consequently it is a disease of economic significance in every country where stone fruits are grown. To achieve successful eradication or effective management of the associated disease, sensitive detection of the virus in infected plants is required. TaqMan PCR Kit Click to expand options PPV TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents PPV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM33450 (100 preps) Cat. TM33410 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - PPV Primer & Probe Mix 280 μL 280 μL PPV Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM33450) PPV TaqMan RT-PCR Kit - Protocol (100 rxns) (TM33410) PPV TaqMan Probe/Primer and Control Set - Protocol (100 rxns) POSTERS Development of a high throughput PPV detection method based on unique nucleic acid isolation system Related Products Plum Pox Virus (PPV) TaqMan Probe/Primer and Control Set Plant/Fungi Total RNA Purification Kit
Plum Pox Virus (PPV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plum Pox Virus (PPV) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM33450 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
