Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
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ProteoSpin™ On-Column Proteolytic Digestion Kit
Rapid and simple procedure to generate digested peptides
Simultaneous digestion, purification and concentration at once
Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry
Peptides are ready for applications such as mass spectrometry and SDS-PAGE
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format. Trypsin is added to the protein sample and bound to the column. Salts are washed away and the trypsin is then activated to digest proteins. Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation). The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.
Figure 1. Generation of TrypticBSA Peptides without the Generation of any Artifacts. MALDI-TOF spectra of the peptides that resulted after BSA was digested on-column with trypsin using the ProteoSpinTMOn-Column Proteolytic Digestion Kit (Panel A) or digested in a typical liquid environment (Panel B). For both digestions, 15 µg of BSA was used as the input and 0.3 µg of Trypsin was used for the digestion. For on-column digestion, the 15 µg of BSA was loaded onto the column with the trypsin, the BSA was washed, then the on-column digestion proceeded and the resulting peptides were eluted. By comparing the spectra, it can be seen that the ProteoSpinTMkit (Panel A) resulted in the generation of the same peptide profile in MALDI-TOF as the liquid digestion did (Panel B). Furthermore, no artifacts were found to arise from the use of the column, and all the same peaks could be detected in both spectra.
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.