ProteoSpin™ On-Column Proteolytic Digestion Kit For the rapid and convenient on-column digestion of proteins For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ProteoSpin™ On-Column Proteolytic Digestion Kit For the rapid and convenient on-column digestion of proteins Price USD$219.00 SKU 17500 Format Spin Column Size 25 Preps Quantity Overview Details Documentation Citations Overview Rapid and simple procedure to generate digested peptides Simultaneous digestion, purification and concentration at once Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry Peptides are ready for applications such as mass spectrometry and SDS-PAGE Purification is based on spin column chromatography that uses Norgen’s resin separation matrix This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format. Trypsin is added to the protein sample and bound to the column. Salts are washed away and the trypsin is then activated to digest proteins. Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation). The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more. Details Supporting Data Previous Figure 1. Generation of Tryptic BSA Peptides without the Generation of any Artifacts. MALDI-TOF spectra of the peptides that resulted after BSA was digested on-column with trypsin using the ProteoSpinTM On-Column Proteolytic Digestion Kit (Panel A) or digested in a typical liquid environment (Panel B). For both digestions, 15 µg of BSA was used as the input and 0.3 µg of Trypsin was used for the digestion. For on-column digestion, the 15 µg of BSA was loaded onto the column with the trypsin, the BSA was washed, then the on-column digestion proceeded and the resulting peptides were eluted. By comparing the spectra, it can be seen that the ProteoSpinTM kit (Panel A) resulted in the generation of the same peptide profile in MALDI-TOF as the liquid digestion did (Panel B). Furthermore, no artifacts were found to arise from the use of the column, and all the same peaks could be detected in both spectra. Next Click for expanded view Kit Specifications Maximum Protein Input 15 μg Minimum Protein Input 2 μg Minimum Elution Volume 30 μL Time to Process 10 Purifications 20 minutes (Plus a 1-hour incubation) Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment. Component Cat. 17500 (25 preps) Wash Solution C 30 mL Binding Buffer A 4 mL Column Activation Buffer 3 mL Micro Spin Columns 25 Collection Tubes 25 Elution tubes (1.7 mL) 25 Product Insert 1 Documentation PROTOCOLS PI17500-12 Proteospin On-Column Proteolytic Digestion Kit.pdf SAFETY DATA SHEETS 17500_SDS.pdf FLYERS Detergent-Free Total Protein Isolation Kit.pdf RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Products ProteoSpin™ Total Protein Concentration and Detergent Clean-Up Mini Kit
ProteoSpin™ On-Column Proteolytic Digestion Kit For the rapid and convenient on-column digestion of proteins For research use only and NOT intended for in vitro diagnostics. Powered by Bioz ProteoSpin™ On-Column Proteolytic Digestion Kit For the rapid and convenient on-column digestion of proteins Price USD$219.00 SKU 17500 Format Spin Column Size 25 Preps Quantity