ProteoSpin™ Inclusion Body Protein Isolation Kits

Cat. 10300, 17700
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ProteoSpin™ Inclusion Body Protein Isolation Kits

Cat. 10300, 17700

For the rapid isolation of inclusion body proteins

  • All-in-one solution for inclusion body protein isolation and purification
  • Fast and convenient spin column protocol
  • Complete kit with Cell Lysis Reagent, Inclusion Body Solubilization Reagent, buffers and spin columns to purify proteins
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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ProteoSpin™ Inclusion Body Isolation Micro Kit
(Cat. 10300)
25 preps
ProteoSpin™ Inclusion Body Isolation Maxi Kit
(Cat. 17700)
4 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • All-in-one solution for inclusion body protein isolation and purification
  • Fast and convenient spin column protocol
  • Complete kit with Cell Lysis Reagent, Inclusion Body Solubilization Reagent, buffers and spin columns to purify proteins
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

ProteoSpin™ Inclusion Body Protein Isolation Kits

These kits provide everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.

ProteoSpin™ Inclusion Body Protein Isolation Micro Kit

The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 µg of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit

The procedure is efficient and streamlined and can process up to 4 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic proteins from 100 mL of induced bacterial culture. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

About Inclusion Bodies

Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are typically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these subcellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example. Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time-consuming and not cost-effective. This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography - all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.

Kit Specifications

You have selected: Cat. 10300
Kit Specifications
Maximum Culture Volume
1.5 mL
Yield from 1.5 mL
Up to 50 μg
Minimum Elution Volume
30 μL
Time to Process 12 Samples
60 minutes

 

Storage Conditions

The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for the pH Binding Buffers (Acidic and Basic). Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.

You have selected: Cat. 17700
Kit Specifications
Maximum Culture Volume
100 mL
Yield from 100 mL of Culture
Up to 12 mg
Minimum Elution Volume
4 mL
Time to Process 1 Sample
1 hour

Storage Conditions
The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for Binding Buffer C and Binding Buffer N. Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.

Supporting Data

Click for expanded view

Component Cat. 10300 (Micro - 25 preps) Cat. 17700 (Maxi - 4 preps)
Wash Solution C 30 mL 130 mL
Wash Solution N 30 mL 130 mL
Binding BUffer A 4 mL 20 mL
Binding Buffer N 4 mL 20 mL
Elution Buffer C 8 mL 2 x 30 mL
Protein Neutralizer 4 mL 4 mL
Cell Lysis Reagent 15 mL 110 mL
IB Solubilization Reagent 2 mL 50 mL
Syringes, 1cc, slip tip 25 -
Needles (Bev, 20G x 1 inch) 25 -
Syringes, 10 mL, Luer-Lok™ Tip - 4
Needles (18G x 1.5 inch) - 4
Micro Spin Columns 25 -
Maxi Spin Columns (filled with SiC) inserted into 50 mL collection tubes - 4
Collection Tubes 25 -
Elution Tubes (1.7 mL) 25 -
Elution Tubes (50 mL) - 4
Product Insert 1 1

Maxi (17700)

Title CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidase
Journal BMC Microbiology. 2016.
Authors Shadnezhad A, Naegeli A, Collin M.
Title Proteome of Human Calcium Kidney Stones.
Journal Urology. 2010.
Authors Canales BK, Anderson L, Higgins L, Ensrud-Bowlin K, Roberts KP, Wu B, Kim IW, Monga M.

ProteoSpin™ Inclusion Body Protein Isolation Kits

These kits provide everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.

ProteoSpin™ Inclusion Body Protein Isolation Micro Kit

The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 µg of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit

The procedure is efficient and streamlined and can process up to 4 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic proteins from 100 mL of induced bacterial culture. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

About Inclusion Bodies

Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are typically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these subcellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example. Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time-consuming and not cost-effective. This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography - all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 10300
Kit Specifications
Maximum Culture Volume
1.5 mL
Yield from 1.5 mL
Up to 50 μg
Minimum Elution Volume
30 μL
Time to Process 12 Samples
60 minutes

 

Storage Conditions

The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for the pH Binding Buffers (Acidic and Basic). Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.

You have selected: Cat. 17700
Kit Specifications
Maximum Culture Volume
100 mL
Yield from 100 mL of Culture
Up to 12 mg
Minimum Elution Volume
4 mL
Time to Process 1 Sample
1 hour

Storage Conditions
The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for Binding Buffer C and Binding Buffer N. Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.

Component Cat. 10300 (Micro - 25 preps) Cat. 17700 (Maxi - 4 preps)
Wash Solution C 30 mL 130 mL
Wash Solution N 30 mL 130 mL
Binding BUffer A 4 mL 20 mL
Binding Buffer N 4 mL 20 mL
Elution Buffer C 8 mL 2 x 30 mL
Protein Neutralizer 4 mL 4 mL
Cell Lysis Reagent 15 mL 110 mL
IB Solubilization Reagent 2 mL 50 mL
Syringes, 1cc, slip tip 25 -
Needles (Bev, 20G x 1 inch) 25 -
Syringes, 10 mL, Luer-Lok™ Tip - 4
Needles (18G x 1.5 inch) - 4
Micro Spin Columns 25 -
Maxi Spin Columns (filled with SiC) inserted into 50 mL collection tubes - 4
Collection Tubes 25 -
Elution Tubes (1.7 mL) 25 -
Elution Tubes (50 mL) - 4
Product Insert 1 1

Maxi (17700)

Title CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidase
Journal BMC Microbiology. 2016.
Authors Shadnezhad A, Naegeli A, Collin M.
Title Proteome of Human Calcium Kidney Stones.
Journal Urology. 2010.
Authors Canales BK, Anderson L, Higgins L, Ensrud-Bowlin K, Roberts KP, Wu B, Kim IW, Monga M.

 


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