ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits

Cat. 22800, 22200
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ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits

Cat. 22800, 22200

For rapid and efficient endotoxin removal from proteins and peptides

  • Columns bind proteins of interest while endotoxins flow through
  • Reduce endotoxin levels to less than 0.01 EU/µg of protein
  • Proteins are desalted
  • Greater than 95% protein recovery
  • Concentrate protein samples and remove detergents at the same time
  • Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit
(Cat. 22800)
25 preps
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit
(Cat. 22200)
4 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Columns bind proteins of interest while endotoxins flow through
  • Reduce endotoxin levels to less than 0.01 EU/µg of protein
  • Proteins are desalted
  • Greater than 95% protein recovery
  • Concentrate protein samples and remove detergents at the same time
  • Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

Mini Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Maxi Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Kit Specifications

You have selected: Cat. 22800
Kit Specifications
Maximum Protein Input
200 μg
Maximum Column Volume Input
600 μL
Molecular Weight of Recovered Proteins No Molecular Weight cut-off
Final Endotoxin Levels
≤ 0.01 EU/μg protein
Protein Recovery
90-95%
% Detergent Removal 90-95%
Detergents that can be Removed Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

You have selected: Cat. 22200
Kit Specifications
Maximum Protein Input
4 mg
Minimum Protein Input
0.25 mg
Maximum Column Volume Input 18 mL
Molecular Weight of Recovered Proteins No Molecular Weight cut-off
Final Endotoxin Levels
≤ 0.01 EU/μg protein
Protein Recovery
90-95%
% Detergent Removal 90-95%
Detergents that can be Removed Including Triton® X-100, CHAPS, NP-40 and Tween 20
Elution Volume 2-4 mL
Time to Complete 10 Purifications
30-40 minutes


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 22800 (25 preps) Cat. 22200 (4 preps)
Binding Buffer J 8 mL 8 mL
Binding Buffer N 4 mL 20 mL
Wash Solution M 50 mL 130 mL
Wash Solution CIP 20 mL 60 mL
Wash Solution N 30 mL 130 mL
Wash Solution NIP 20 mL 60 mL
Elution Buffer G 6 mL 20 mL
Endotoxin Removal Solution 1.5 mL 1.5 mL
Protein Neutralizer EF 2 mL 2 mL
Maxi Spin Columns (assembled with Collection Tubes) - 4
Mini Spin Columns 25 -
Collection Tubes 25 -
Maxi Spin Columns (assembled with Collection Tubes) 4
Elution Tubes (1.7 mL) 25 -
Elution Tubes (50 mL) 4 -
Product Insert 1 1

Mini (22800)

Title The emetic activity of staphylococcal enterotoxins, SEK, SEL, SEM, SEN and SEO, in a small emetic animal model, house musk shrew
Journal Microbiology and Immunology. 2017.
Authors Hisaya K. Ono, Shouhei Hirose, Ikunori Naito, Yusuke Sato'o, Krisana Asano, Dong-Liang Hu, Katsuhiko Omoe, Akio Nakane
Title HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice
Journal Journal of Molecular Microbiology and Biotechnology. 2016.
Authors Kang X, Yang Y, Jiao Y, Song H, Song L, Xiong D, Wu L, Pan Z, Jiao X.
Title Nucleic acids encodng anti-C5A antibodies
Journal United States Patents US9434784 B1. 2016.
Authors Russell P. Rother, Douglas L. Sheridan, Paul P. Tamburini, Yuchun Zhang
Title Expression of recombinant Newcastle disease virus F protein in Pichia pastoris and its immunogenicity using flagellin as the adjuvant
Journal Protein Expression and Purification. 2016.
Authors Kang X, Wang J, Jiao Y, Tang P, Song L, Xiong D, Yin Y, Pan Z, Jiao X
Title Synthetic Biology Platform for Sensing and Integrating Endogenous Transcriptional Inputs in Mammalian Cells
Journal Cell Reports. 2016.
Authors Angelici, B., Mailand, E., Haefliger, B., & Benenson, Y
Title Recombinant IL-4/13A and IL-4/13B induce arginase activity and down-regulate nitric oxide response of primary goldfish (Carassius auratus L.) macrophages
Journal Developmental & Comparative Immunology. 2016.
Authors Hodgkinson JW, Fibke C, Belosevic M
Title ANTI-C5 Antibodies Having Improved Pharmacokinetics
Journal United States Patent Application 20160108115. 2016.
Authors Andrien Jr., Bruce A., Sheridan, Douglas L., Tamburini, Paul P.
Title Methods and Compositions for Treating Cancer and Inflammatory Diseases
Journal United States Patent Application 20160159872. 2016.
Authors Zhang Q, Zhao R, Wang XJ, Blevins M
Title Anti-C5A Antibodies
Journal United States Patent Application 20160159892. 2016.
Authors Rother RP, Sheridan DL, Tamburini PP, Zhang Y
Title Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages
Journal Jundishapur Journal of Microbiology. 2016.
Authors Xie, X., Han, M., Zhang, L., Liu, L., Gu, Z., Yang, M., & Yang, H
Title Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Also Exhibit Chaperone like Activity In-Vitro and In-Vivo
Journal PLOS ONE. 2016.
Authors Pandey S, Sharma A, Tripathi D, Kumar A, Khubaib M, Bhuwan M, Chaudhuri TK, Hasnain SE, Ehtesham NZ
Title Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori
Journal Frontiers in Microbiology. 2016.
Authors Hanafi A, Lee WC, Loke MF, Teh X, Shaari A, Dinarvand M, Lehours P, Mégraud F, Leow AH, Vadivelu J, Goh KL.
Title Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.
Journal Glia. 2015.
Authors Levine J, Kwon E, Paez P, Yan W, Czerwieniec G, Loo JA, Sofroniew MV6, Wanner IB
Title Substitution in Amino Acid 70 of Hepatitis C Virus Core Protein Changes the Adipokine Profile via Toll-Like Receptor 2/4 Signaling
Journal PLOS ONE. 2015.
Authors Satoko Uraki, Masahiko Tameda, Kazushi Sugimoto, Katsuya Shiraki, Yoshiyuki Takei, Tsutomu Nobori, Masaaki Ito
Title Inhibition of 4-1BBL-regulated TLR response in macrophages ameliorates endotoxin-induced sepsis in mice
Journal European Journal of Immunology. 2014.
Authors Bo Ram Bang, Sang Jick Kim, Hideo Yagita, Michael Croft and Young Jun Kang
Title Immunological characteristics of Mycobacterium tuberculosis antigen Rv2628
Journal Chinese Journal of Biotechnology. 2014.
Authors Yin Y, Gao Y, Zhao D, Lian K, Chen X, Xu Z, Pan Z and Jiao X.
Title Passive Immunization with Tau Oligomer Monoclonal Antibody Reverses Tauopathy Phenotypes without Affecting Hyperphosphorylated Neurofibrillary Tangles.
Journal Journal of Neuroscience. 2014.
Authors Castillo-Carranza DL, Sengupta U, Guerrero-Muñoz MJ, Lasagna-Reeves CA, Gerson JE, Singh G, Estes DM, Barrett AD, Dineley KT, Jackson GR, Kayed R.
Title Identification and functional characterization of the goldfish (Carassius auratus L.) high mobility group box 1 (HMGB1) chromatin-binding protein.
Journal Developmental and Comparative Immunology. 2014.
Authors Xie J, Hodgkinson JW, Li C, Kovacevic N, Belosevic M.
Title Cross-protective effect of a novel multi-antigen-chimeric vaccine against Streptococcus and Staphylococcus aureus infection in mice.
Journal Journal of Medical Microbiology. 2014.
Authors Yu L, Fan Z, Ma J, Tong C, Song B, Zhu Z, Cui Y.
Title Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase.
Journal Nature Neuroscience. 2014.
Authors Andrei I Molosh, Philip L Johnson, John P Spence, David Arendt, Lauren M Federici, Cristian Bernabe, Steven P Janasik, Zaneer M Segu, Rajesh Khanna, Chirayu Goswami, Weiguo Zhu, Su-Jung Park, Lang Li, Yehia S Mechref, D Wade Clapp & Anantha Shekhar.
Title Prolactin fractions from lactating rats elicit effects upon sensory spinal cord cells of male rats.
Journal Neuroscience. 2013.
Authors Mena F, González-Hernández A, Navarro N, Castilla A, Morales T, Rojas-Piloni G, Martínez-Lorenzana G, Lara MC.
Title Improved protective efficacy of a chimeric staphylococcus aureus vaccine candidate.
Journal Microbiology and Immunology2013.
Authors Yu L, Wang N, Ma J, Tong C, Song B, Chi J, Ma G, Zhu Z, Cui Y.
Title Borrelia burgdorferi BbHtrA degrades host ECM proteins and stimulates release of inflammatory cytokines in vitro.
Journal Molecular Microbiology. 2013.
Authors Russell TM, Delorey MJ, Johnson BJ.
Title FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling.
Journal Proceedings of the National Academy of Sciences of the United States of America. 2013.
Authors Zhu Z, Li R, Li H, Zhou T, Davis RS.
Title Variant screening of the serum amyloid A1 gene and functional study of the p.Gly90Asp variant for its role in atherosclerosis.
Journal Atherosclerosis. 2013.
Authors Leow KY, Goh WW, Tan SZ, Lim J, Ng K, Oh VM, Low AF, Heng CK.
Title The Toll-like receptor 5 ligand flagellin promotes asthma by priming allergic responses to indoor allergens.
Journal Nature Medicine2012.
Authors Wilson RH, Maruoka S, Whitehead GS, Foley JF, Flake GP, Sever ML, Zeldin DC, Kraft M, Garantziotis S, Nakano H, Cook DN.
Title Quantitative retinal protein analysis using iTRAQ tags after optic nerve transection reveals a neuroprotective role for hepatoma derived growth factor.
Journal Investigative Ophthamology & Visual Science. 2012.
Authors Hollander A, D'Onofrio PM, Magharious MM, Lysko MD, Koeberle PD.
Title Serum low-molecular-weight protein fractionation for biomarker discovery.
Journal Methods in Molecular Biology. 2011.
Authors Vanmeter AJ, Camerini S, Polci ML, Tessitore A, Trivedi N, Heiby M, Kamal Y, Hansen J, Zhou W.
Title Active and Passive Immunization with rHyr1p-N Protects Mice against Hematogenously Disseminated Candidiasis.
Journal PLOS One. 2011.
Authors Luo G, Ibrahim A, French S, Edwards J Jr and Fu Y.
Title Galectin-9 and T Cell Immunoglobulin Mucin-3 Pathway Is a Therapeutic Target for Type 1 Diabetes.
Journal Endocrinology2011.
Authors Kanzaki M, Wada J, Sugiyama K, Nakatsuka A, Teshigawara S, Murakami K, Inoue K, Terami T, Katayama A, Eguchi J, Akiba H, Yagita H, Makino H.
Title Generation of Bivalent and Bispecific Kringle Single Domains by Loop Grafting as Potent Agonists against Death Receptors 4 and 5.
Journal Journal of Molecular Biology. 2011.
Authors Lee CH, Park KJ, Kim SJ, Kwon O, Jeong KJ, Kim A, Kim YS.
Title ApoB-100 and HSP60 Peptides Exert a Synergetic Role in Inhibiting Early Atherosclerosis in Immunized ApoE-null Mice.
Journal Protein and Peptide Letters. 2011.
Authors Li J, Zhao X, Zhang S, Wang S, Du P, Qi G.
Title Disparities in TLR5 Expression and Responsiveness to Flagellin in Equine Neutrophils and Mononuclear Phagocytes.
Journal Journal of Immunology. 2011.
Authors Kwon S, Gewirtz AT, Hurley DJ, Robertson TP, Moore JN, Vandenplas ML.
Title Comparative Effects of Toll-Like Receptor Agonists on a Low Dose PspA Intranasal Vaccine against Fatal Pneumococcal Pneumonia in Mice.
Journal Journal of Vaccines and Vaccination. 2011.
Authors Piao Z, Oma K, Ezoe H, Akeda Y, Tomono K, Oishi K.
Title Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood.
Journal Biomaterials. 2011.
Authors Xu H, Aguilar ZP, Yang L, Kuang M, Duan H, Xiong Y, Wei H, Wang A.
Title Quantitative iTRAQ analysis of retinal ganglion cell degeneration after optic nerve crush.
Journal Journal of Proteome Research. 2011.
Authors Magharious M, D'Onofrio PM, Hollander A, Zhu P, Chen J, Koeberle PD.
Title Use of capillary electrophoresis-sodium dodecyl sulfate to monitor disulfide scrambled forms of an Fc fusion protein during purification process.
Journal Analytical Biochemistry. 2011.
Authors Hapuarachchi S, Fodor S, Apostol I, Huang G.
Title Proteomics Analysis of Human Optic Nerve Head Astrocytes Following Biomechanical Strain.
Journal Molecular and Cellular Proteonomics. 2011.
Authors Rogers RS, Dharsee M, Ackloo S, Sivak JM, Flanagan JG.
Title Computational Design of Epitope-Scaffolds Allows Induction of Antibodies Specific for a Poorly Immunogenic HIV Vaccine Epitope
Journal Structure. Vol 18, 2010.
Authors Correia E, Ban Y, Holmes M, Xu H, Ellingson K, Kraft Z, Carrico C, Boni E, Sather D, Zenobia C, Burke K, Bradley-Hewitt T, Bruhn-Johannsen J, Kalyuzhniy O, Baker D, Strong R, Stamatatos L, Schief1 W
Title Myeloid Differentiation Primary Response Protein 88 Couples Reverse Cholesterol Transport to Inflammation.
Journal J Allergy Clin Immunol. 2010.
Authors Conway DH, Dara J, Bagashev A, Sullivan KE.
Title The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins.
Journal The Journal of Allergy and Clinical Immunology. 2010.
Authors Mueller GA, Edwards LL, Aloor JJ, Fessler MB, Glesner J, Pomés A, Chapman MD, London RE, Pedersen LC.
Title An aberrant prostate antigen–specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans
Journal Journal of Clinical Investigation. 2009.
Authors Hou Y, DeVoss J, Dao V, Kwek S, Simko JP, McNeel DG, Anderson MS, Fong L.
Title Intranasal immunization with a mixture of PspA and a Toll-like receptor agonist induces specific antibodies and enhances bacterial clearance in the airways of mice
Journal Vaccine. 2009.
Authors Oma K, Zhao J, Ezoe H, Akeda Y, Koyama S, Ishii KJ, Kataoka K, Oishi K.
Title Molecular cloning and functional characterization of avian interleukin-19.
Journal Molecular Immunology 47(2-3):476-842009.
Authors Kim S, Miska KB, McElroy AP, Jenkins MC, Fetterer RH, Cox CM, Stuard LH, Dalloul RA.
Title Sample preparation and fractionation for proteome analysis and cancer biomarker discovery by mass spectrometry.
Journal Journal of Separation Science. 2009.
Authors Farid E. Ahmed.
Title Proteomic analysis of a matrix stone: a case report.
Journal Urology Research. 2009.
Authors Canales BK, Anderson L, Higgins L, Frethem C, Ressler A, Kim IW, Monga M.
Title Comprehensive Proteomic Analysis of Human Calcium Oxalate Monohydrate Kidney Stone Matrix.
Journal Journal of Endourology. 2008.
Authors Anderson L, Canales B, Higgins L, Liu N, Monga M, Roberts K, Slaton J.
Title Immunotherapy of hepatocellular carcinoma with a vaccine based on xenogeneic homologous a fetoprotein in mice
Journal Biochemical and Biophysical Research Communications. 2008.
Authors Zhang W, Liu J, Wu Y, Xiao F, Wang Y, Wang R, Yang H, Wang G, Yang J, Deng H, Li J, Wen Y, Wei Y.
Title Development and Characterization of a TAPIR-Like Mouse Monoclonal Antibody to Amyloid-beta
Journal Journal of Alzheimer's Disease.Vol 4, no 2, 161-1732008.
Authors Wang J, Hara H, Makifuchi T, Tabira T.
Title Electrophoretic Analysis of Proteins Isolated From Mammalian, Avian, and Reptilian Bone Using a Pressure Cycling
Journal 9th Annual Symposium on Advances in Separation Sciences and Mass Spectrometry Poster. 2007.
Authors Smejkal G, Kwan A, Muddiman D, Schweitzer M, Collier T.
Title Identification of eggshell membrane proteins and purification of ovotransferrin and beta-NAGase from hen egg white.
Journal Protein Journal.. 2006.
Authors Ahlborn GJ, Clare DA, Sheldon BW, Kelly RW.

Maxi (22200)

Title ANTI-C5 Antibodies Having Improved Pharmacokinetics
Journal United States Patent Application 20160108115. 2016.
Authors Andrien Jr., Bruce A., Sheridan, Douglas L., Tamburini, Paul P.
Title Methods and Compositions for Treating Cancer and Inflammatory Diseases
Journal United States Patent Application 20160159872. 2016.
Authors Zhang Q, Zhao R, Wang XJ, Blevins M
Title Anti-C5A Antibodies
Journal United States Patent Application 20160159892. 2016.
Authors Rother RP, Sheridan DL, Tamburini PP, Zhang Y
Title Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Also Exhibit Chaperone like Activity In-Vitro and In-Vivo
Journal PLOS ONE. 2016.
Authors Pandey S, Sharma A, Tripathi D, Kumar A, Khubaib M, Bhuwan M, Chaudhuri TK, Hasnain SE, Ehtesham NZ
Title An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin
Journal BMC Biotechnology. 2015.
Authors Li Song, Dan Xiong, Xilong Kang, Yun Yang, Jing Wang, Yaxin Guo, Hui Xu, Sujuan Chen, Daxin Peng, Zhiming Pan and Xinan Jiao
Title Identification and Characterization of a Novel Staphylococcal Emetic Toxin
Journal Applied and Environmental Microbiology. 2015.
Authors Hisaya K. Ono, Yusuke Sato'o, Kouji Narita, Ikunori Naito, Shouhei Hirose, Junzo Hisatsune, Krisana Asano, Dong-Liang Hu, Katsuhiko Omoe, Motoyuki Sugai and Akio Nakane
Title A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB.
Journal BMC Veterinary Research. 2015.
Authors Dan Xiong, Li Song, Xianyue Zhai, Shizhong Geng, Zhiming Pan and Xinan Jiao.
Title Amino acids 89–96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response.
Journal Cellular & Molecular Immunology. 2014.
Authors Lei Zhang, Zhiming Pan, Xilong Kang, Yun Yang, Heekap Kang, Na Zhang, James M Rosati and Xinan Jiao.
Title Pulmonary CD103+ dendritic cells prime Th2 responses to inhaled allergens.
Journal Mucosal Immunology. 2011.
Authors Nakano H, Free M, Whitehead G, Maruoka S, Wilson R, Nakano K and Cook D.
Title A Novel Core Genome-Encoded Superantigen Contributes to Lethality of Community-Associated MRSA Necrotizing Pneumonia.
Journal PLoS Pathogens. 2011.
Authors Wilson G, Seo KS, Cartwright R, Connelley T, Chuang-Smith O, Merriman J, Guinane C, Park J, Bohach G, Schlievert P, Morrison W and Fitzgerald J.
Title An Optimized Approach for Protein Residue Extraction and Identification from Ceramics After Cooking.
Journal Journal of Archaeological Method and Theory. 2011.
Authors Barker A, Venables B, Stevens S, Seeley K, Wang P and Wolverton S
Title Two-dimensional electrophoresis and IgE-mediated food allergy.
Journal Electrophoresis. 2010.
Authors De Angelis M, Di Cagno R, Minervini F, Rizzello CG, Gobbetti M.
Title Proteome of Human Calcium Kidney Stones.
Journal Urology. 2010.
Authors Canales BK, Anderson L, Higgins L, Ensrud-Bowlin K, Roberts KP, Wu B, Kim IW, Monga M.
Title Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli.
Journal Peptides. 2010.
Authors Bommarius B, Jenssen H, Elliott M, Kindrachuk J, Pasupuleti M, Gieren H, Jaeger KE, Hancock RE, Kalman D.
Title An aberrant prostate antigen?specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans
Journal Journal of Clinical Investigation. 2009.
Authors Hou Y, DeVoss J, Dao V, Kwek S, Simko JP, McNeel DG, Anderson MS, Fong L.
Title Structural stability of Amandin, a major allergen from almond (Prunus dulcis), and its acidic and basic polypeptides.
Journal Journal of Agriculture and Food Chemistry. 2009.
Authors Albillos SM, Menhart N, Fu TJ.
Title Comprehensive Proteomic Analysis of Human Calcium Oxalate Monohydrate Kidney Stone Matrix
Journal Journal of Endourology. 2008.
Authors Benjamin K. Canales, Lorraine Anderson, Leeann Higgins, Joel Slaton, Ken P. Roberts, Nathan Liu, Manoj Monga.
Title Development and Characterization of a TAPIR-Like Mouse Monoclonal Antibody to Amyloid-beta
Journal Journal of Alzheimer's Disease.Vol 4, no 2, 161-173. 2008.
Authors Wang J, Hara H, Makifuchi T, Tabira T.
Title Immunotherapy of hepatocellular carcinoma with a vaccine based on xenogeneic homologous a fetoprotein in mice
Journal Biochemical and Biophysical Research Communications. 2008.
Authors Zhang W, Liu J, Wu Y, Xiao F, Wang Y, Wang R, Yang H, Wang G, Yang J, Deng H, Li J, Wen Y, Wei Y.
Title A method for the selective isolation and enrichment of carrier protein-bound low-molecular weight proteins and peptides in the blood.
Journal Proteomics Clinical Applications. 2007.
Authors Serena Camerini, Maria Letizia Polci, Lance A. Liotta, Emanuel F. Petricoin, Weidong Zhou.
Title Identification of eggshell membrane proteins and purification of ovotransferrin and beta-NAGase from hen egg white.
Journal Protein Journal. 2006.
Authors Ahlborn GJ, Clare DA, Sheldon BW, Kelly RW.
Title A strategy for high-resolution protein identification in surface-enhanced laser desorption/ionization mass spectrometry: calgranulin A and chaperonin 10 as protein markers for endometrial carcinoma.
Journal Proteomics. 2005.
Authors Guo J, Yang EC, Desouza L, Diehl G, Rodrigues MJ, Romaschin AD, Colgan TJ, Siu KW.

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

Mini Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Maxi Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 22800
Kit Specifications
Maximum Protein Input
200 μg
Maximum Column Volume Input
600 μL
Molecular Weight of Recovered Proteins No Molecular Weight cut-off
Final Endotoxin Levels
≤ 0.01 EU/μg protein
Protein Recovery
90-95%
% Detergent Removal 90-95%
Detergents that can be Removed Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.

You have selected: Cat. 22200
Kit Specifications
Maximum Protein Input
4 mg
Minimum Protein Input
0.25 mg
Maximum Column Volume Input 18 mL
Molecular Weight of Recovered Proteins No Molecular Weight cut-off
Final Endotoxin Levels
≤ 0.01 EU/μg protein
Protein Recovery
90-95%
% Detergent Removal 90-95%
Detergents that can be Removed Including Triton® X-100, CHAPS, NP-40 and Tween 20
Elution Volume 2-4 mL
Time to Complete 10 Purifications
30-40 minutes


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component Cat. 22800 (25 preps) Cat. 22200 (4 preps)
Binding Buffer J 8 mL 8 mL
Binding Buffer N 4 mL 20 mL
Wash Solution M 50 mL 130 mL
Wash Solution CIP 20 mL 60 mL
Wash Solution N 30 mL 130 mL
Wash Solution NIP 20 mL 60 mL
Elution Buffer G 6 mL 20 mL
Endotoxin Removal Solution 1.5 mL 1.5 mL
Protein Neutralizer EF 2 mL 2 mL
Maxi Spin Columns (assembled with Collection Tubes) - 4
Mini Spin Columns 25 -
Collection Tubes 25 -
Maxi Spin Columns (assembled with Collection Tubes) 4
Elution Tubes (1.7 mL) 25 -
Elution Tubes (50 mL) 4 -
Product Insert 1 1

Mini (22800)

Title The emetic activity of staphylococcal enterotoxins, SEK, SEL, SEM, SEN and SEO, in a small emetic animal model, house musk shrew
Journal Microbiology and Immunology. 2017.
Authors Hisaya K. Ono, Shouhei Hirose, Ikunori Naito, Yusuke Sato'o, Krisana Asano, Dong-Liang Hu, Katsuhiko Omoe, Akio Nakane
Title HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice
Journal Journal of Molecular Microbiology and Biotechnology. 2016.
Authors Kang X, Yang Y, Jiao Y, Song H, Song L, Xiong D, Wu L, Pan Z, Jiao X.
Title Nucleic acids encodng anti-C5A antibodies
Journal United States Patents US9434784 B1. 2016.
Authors Russell P. Rother, Douglas L. Sheridan, Paul P. Tamburini, Yuchun Zhang
Title Expression of recombinant Newcastle disease virus F protein in Pichia pastoris and its immunogenicity using flagellin as the adjuvant
Journal Protein Expression and Purification. 2016.
Authors Kang X, Wang J, Jiao Y, Tang P, Song L, Xiong D, Yin Y, Pan Z, Jiao X
Title Synthetic Biology Platform for Sensing and Integrating Endogenous Transcriptional Inputs in Mammalian Cells
Journal Cell Reports. 2016.
Authors Angelici, B., Mailand, E., Haefliger, B., & Benenson, Y
Title Recombinant IL-4/13A and IL-4/13B induce arginase activity and down-regulate nitric oxide response of primary goldfish (Carassius auratus L.) macrophages
Journal Developmental & Comparative Immunology. 2016.
Authors Hodgkinson JW, Fibke C, Belosevic M
Title ANTI-C5 Antibodies Having Improved Pharmacokinetics
Journal United States Patent Application 20160108115. 2016.
Authors Andrien Jr., Bruce A., Sheridan, Douglas L., Tamburini, Paul P.
Title Methods and Compositions for Treating Cancer and Inflammatory Diseases
Journal United States Patent Application 20160159872. 2016.
Authors Zhang Q, Zhao R, Wang XJ, Blevins M
Title Anti-C5A Antibodies
Journal United States Patent Application 20160159892. 2016.
Authors Rother RP, Sheridan DL, Tamburini PP, Zhang Y
Title Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages
Journal Jundishapur Journal of Microbiology. 2016.
Authors Xie, X., Han, M., Zhang, L., Liu, L., Gu, Z., Yang, M., & Yang, H
Title Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Also Exhibit Chaperone like Activity In-Vitro and In-Vivo
Journal PLOS ONE. 2016.
Authors Pandey S, Sharma A, Tripathi D, Kumar A, Khubaib M, Bhuwan M, Chaudhuri TK, Hasnain SE, Ehtesham NZ
Title Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori
Journal Frontiers in Microbiology. 2016.
Authors Hanafi A, Lee WC, Loke MF, Teh X, Shaari A, Dinarvand M, Lehours P, Mégraud F, Leow AH, Vadivelu J, Goh KL.
Title Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.
Journal Glia. 2015.
Authors Levine J, Kwon E, Paez P, Yan W, Czerwieniec G, Loo JA, Sofroniew MV6, Wanner IB
Title Substitution in Amino Acid 70 of Hepatitis C Virus Core Protein Changes the Adipokine Profile via Toll-Like Receptor 2/4 Signaling
Journal PLOS ONE. 2015.
Authors Satoko Uraki, Masahiko Tameda, Kazushi Sugimoto, Katsuya Shiraki, Yoshiyuki Takei, Tsutomu Nobori, Masaaki Ito
Title Inhibition of 4-1BBL-regulated TLR response in macrophages ameliorates endotoxin-induced sepsis in mice
Journal European Journal of Immunology. 2014.
Authors Bo Ram Bang, Sang Jick Kim, Hideo Yagita, Michael Croft and Young Jun Kang
Title Immunological characteristics of Mycobacterium tuberculosis antigen Rv2628
Journal Chinese Journal of Biotechnology. 2014.
Authors Yin Y, Gao Y, Zhao D, Lian K, Chen X, Xu Z, Pan Z and Jiao X.
Title Passive Immunization with Tau Oligomer Monoclonal Antibody Reverses Tauopathy Phenotypes without Affecting Hyperphosphorylated Neurofibrillary Tangles.
Journal Journal of Neuroscience. 2014.
Authors Castillo-Carranza DL, Sengupta U, Guerrero-Muñoz MJ, Lasagna-Reeves CA, Gerson JE, Singh G, Estes DM, Barrett AD, Dineley KT, Jackson GR, Kayed R.
Title Identification and functional characterization of the goldfish (Carassius auratus L.) high mobility group box 1 (HMGB1) chromatin-binding protein.
Journal Developmental and Comparative Immunology. 2014.
Authors Xie J, Hodgkinson JW, Li C, Kovacevic N, Belosevic M.
Title Cross-protective effect of a novel multi-antigen-chimeric vaccine against Streptococcus and Staphylococcus aureus infection in mice.
Journal Journal of Medical Microbiology. 2014.
Authors Yu L, Fan Z, Ma J, Tong C, Song B, Zhu Z, Cui Y.
Title Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase.
Journal Nature Neuroscience. 2014.
Authors Andrei I Molosh, Philip L Johnson, John P Spence, David Arendt, Lauren M Federici, Cristian Bernabe, Steven P Janasik, Zaneer M Segu, Rajesh Khanna, Chirayu Goswami, Weiguo Zhu, Su-Jung Park, Lang Li, Yehia S Mechref, D Wade Clapp & Anantha Shekhar.
Title Prolactin fractions from lactating rats elicit effects upon sensory spinal cord cells of male rats.
Journal Neuroscience. 2013.
Authors Mena F, González-Hernández A, Navarro N, Castilla A, Morales T, Rojas-Piloni G, Martínez-Lorenzana G, Lara MC.
Title Improved protective efficacy of a chimeric staphylococcus aureus vaccine candidate.
Journal Microbiology and Immunology2013.
Authors Yu L, Wang N, Ma J, Tong C, Song B, Chi J, Ma G, Zhu Z, Cui Y.
Title Borrelia burgdorferi BbHtrA degrades host ECM proteins and stimulates release of inflammatory cytokines in vitro.
Journal Molecular Microbiology. 2013.
Authors Russell TM, Delorey MJ, Johnson BJ.
Title FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling.
Journal Proceedings of the National Academy of Sciences of the United States of America. 2013.
Authors Zhu Z, Li R, Li H, Zhou T, Davis RS.
Title Variant screening of the serum amyloid A1 gene and functional study of the p.Gly90Asp variant for its role in atherosclerosis.
Journal Atherosclerosis. 2013.
Authors Leow KY, Goh WW, Tan SZ, Lim J, Ng K, Oh VM, Low AF, Heng CK.
Title The Toll-like receptor 5 ligand flagellin promotes asthma by priming allergic responses to indoor allergens.
Journal Nature Medicine2012.
Authors Wilson RH, Maruoka S, Whitehead GS, Foley JF, Flake GP, Sever ML, Zeldin DC, Kraft M, Garantziotis S, Nakano H, Cook DN.
Title Quantitative retinal protein analysis using iTRAQ tags after optic nerve transection reveals a neuroprotective role for hepatoma derived growth factor.
Journal Investigative Ophthamology & Visual Science. 2012.
Authors Hollander A, D'Onofrio PM, Magharious MM, Lysko MD, Koeberle PD.
Title Serum low-molecular-weight protein fractionation for biomarker discovery.
Journal Methods in Molecular Biology. 2011.
Authors Vanmeter AJ, Camerini S, Polci ML, Tessitore A, Trivedi N, Heiby M, Kamal Y, Hansen J, Zhou W.
Title Active and Passive Immunization with rHyr1p-N Protects Mice against Hematogenously Disseminated Candidiasis.
Journal PLOS One. 2011.
Authors Luo G, Ibrahim A, French S, Edwards J Jr and Fu Y.
Title Galectin-9 and T Cell Immunoglobulin Mucin-3 Pathway Is a Therapeutic Target for Type 1 Diabetes.
Journal Endocrinology2011.
Authors Kanzaki M, Wada J, Sugiyama K, Nakatsuka A, Teshigawara S, Murakami K, Inoue K, Terami T, Katayama A, Eguchi J, Akiba H, Yagita H, Makino H.
Title Generation of Bivalent and Bispecific Kringle Single Domains by Loop Grafting as Potent Agonists against Death Receptors 4 and 5.
Journal Journal of Molecular Biology. 2011.
Authors Lee CH, Park KJ, Kim SJ, Kwon O, Jeong KJ, Kim A, Kim YS.
Title ApoB-100 and HSP60 Peptides Exert a Synergetic Role in Inhibiting Early Atherosclerosis in Immunized ApoE-null Mice.
Journal Protein and Peptide Letters. 2011.
Authors Li J, Zhao X, Zhang S, Wang S, Du P, Qi G.
Title Disparities in TLR5 Expression and Responsiveness to Flagellin in Equine Neutrophils and Mononuclear Phagocytes.
Journal Journal of Immunology. 2011.
Authors Kwon S, Gewirtz AT, Hurley DJ, Robertson TP, Moore JN, Vandenplas ML.
Title Comparative Effects of Toll-Like Receptor Agonists on a Low Dose PspA Intranasal Vaccine against Fatal Pneumococcal Pneumonia in Mice.
Journal Journal of Vaccines and Vaccination. 2011.
Authors Piao Z, Oma K, Ezoe H, Akeda Y, Tomono K, Oishi K.
Title Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood.
Journal Biomaterials. 2011.
Authors Xu H, Aguilar ZP, Yang L, Kuang M, Duan H, Xiong Y, Wei H, Wang A.
Title Quantitative iTRAQ analysis of retinal ganglion cell degeneration after optic nerve crush.
Journal Journal of Proteome Research. 2011.
Authors Magharious M, D'Onofrio PM, Hollander A, Zhu P, Chen J, Koeberle PD.
Title Use of capillary electrophoresis-sodium dodecyl sulfate to monitor disulfide scrambled forms of an Fc fusion protein during purification process.
Journal Analytical Biochemistry. 2011.
Authors Hapuarachchi S, Fodor S, Apostol I, Huang G.
Title Proteomics Analysis of Human Optic Nerve Head Astrocytes Following Biomechanical Strain.
Journal Molecular and Cellular Proteonomics. 2011.
Authors Rogers RS, Dharsee M, Ackloo S, Sivak JM, Flanagan JG.
Title Computational Design of Epitope-Scaffolds Allows Induction of Antibodies Specific for a Poorly Immunogenic HIV Vaccine Epitope
Journal Structure. Vol 18, 2010.
Authors Correia E, Ban Y, Holmes M, Xu H, Ellingson K, Kraft Z, Carrico C, Boni E, Sather D, Zenobia C, Burke K, Bradley-Hewitt T, Bruhn-Johannsen J, Kalyuzhniy O, Baker D, Strong R, Stamatatos L, Schief1 W
Title Myeloid Differentiation Primary Response Protein 88 Couples Reverse Cholesterol Transport to Inflammation.
Journal J Allergy Clin Immunol. 2010.
Authors Conway DH, Dara J, Bagashev A, Sullivan KE.
Title The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins.
Journal The Journal of Allergy and Clinical Immunology. 2010.
Authors Mueller GA, Edwards LL, Aloor JJ, Fessler MB, Glesner J, Pomés A, Chapman MD, London RE, Pedersen LC.
Title An aberrant prostate antigen–specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans
Journal Journal of Clinical Investigation. 2009.
Authors Hou Y, DeVoss J, Dao V, Kwek S, Simko JP, McNeel DG, Anderson MS, Fong L.
Title Intranasal immunization with a mixture of PspA and a Toll-like receptor agonist induces specific antibodies and enhances bacterial clearance in the airways of mice
Journal Vaccine. 2009.
Authors Oma K, Zhao J, Ezoe H, Akeda Y, Koyama S, Ishii KJ, Kataoka K, Oishi K.
Title Molecular cloning and functional characterization of avian interleukin-19.
Journal Molecular Immunology 47(2-3):476-842009.
Authors Kim S, Miska KB, McElroy AP, Jenkins MC, Fetterer RH, Cox CM, Stuard LH, Dalloul RA.
Title Sample preparation and fractionation for proteome analysis and cancer biomarker discovery by mass spectrometry.
Journal Journal of Separation Science. 2009.
Authors Farid E. Ahmed.
Title Proteomic analysis of a matrix stone: a case report.
Journal Urology Research. 2009.
Authors Canales BK, Anderson L, Higgins L, Frethem C, Ressler A, Kim IW, Monga M.
Title Comprehensive Proteomic Analysis of Human Calcium Oxalate Monohydrate Kidney Stone Matrix.
Journal Journal of Endourology. 2008.
Authors Anderson L, Canales B, Higgins L, Liu N, Monga M, Roberts K, Slaton J.
Title Immunotherapy of hepatocellular carcinoma with a vaccine based on xenogeneic homologous a fetoprotein in mice
Journal Biochemical and Biophysical Research Communications. 2008.
Authors Zhang W, Liu J, Wu Y, Xiao F, Wang Y, Wang R, Yang H, Wang G, Yang J, Deng H, Li J, Wen Y, Wei Y.
Title Development and Characterization of a TAPIR-Like Mouse Monoclonal Antibody to Amyloid-beta
Journal Journal of Alzheimer's Disease.Vol 4, no 2, 161-1732008.
Authors Wang J, Hara H, Makifuchi T, Tabira T.
Title Electrophoretic Analysis of Proteins Isolated From Mammalian, Avian, and Reptilian Bone Using a Pressure Cycling
Journal 9th Annual Symposium on Advances in Separation Sciences and Mass Spectrometry Poster. 2007.
Authors Smejkal G, Kwan A, Muddiman D, Schweitzer M, Collier T.
Title Identification of eggshell membrane proteins and purification of ovotransferrin and beta-NAGase from hen egg white.
Journal Protein Journal.. 2006.
Authors Ahlborn GJ, Clare DA, Sheldon BW, Kelly RW.

Maxi (22200)

Title ANTI-C5 Antibodies Having Improved Pharmacokinetics
Journal United States Patent Application 20160108115. 2016.
Authors Andrien Jr., Bruce A., Sheridan, Douglas L., Tamburini, Paul P.
Title Methods and Compositions for Treating Cancer and Inflammatory Diseases
Journal United States Patent Application 20160159872. 2016.
Authors Zhang Q, Zhao R, Wang XJ, Blevins M
Title Anti-C5A Antibodies
Journal United States Patent Application 20160159892. 2016.
Authors Rother RP, Sheridan DL, Tamburini PP, Zhang Y
Title Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Also Exhibit Chaperone like Activity In-Vitro and In-Vivo
Journal PLOS ONE. 2016.
Authors Pandey S, Sharma A, Tripathi D, Kumar A, Khubaib M, Bhuwan M, Chaudhuri TK, Hasnain SE, Ehtesham NZ
Title An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin
Journal BMC Biotechnology. 2015.
Authors Li Song, Dan Xiong, Xilong Kang, Yun Yang, Jing Wang, Yaxin Guo, Hui Xu, Sujuan Chen, Daxin Peng, Zhiming Pan and Xinan Jiao
Title Identification and Characterization of a Novel Staphylococcal Emetic Toxin
Journal Applied and Environmental Microbiology. 2015.
Authors Hisaya K. Ono, Yusuke Sato'o, Kouji Narita, Ikunori Naito, Shouhei Hirose, Junzo Hisatsune, Krisana Asano, Dong-Liang Hu, Katsuhiko Omoe, Motoyuki Sugai and Akio Nakane
Title A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB.
Journal BMC Veterinary Research. 2015.
Authors Dan Xiong, Li Song, Xianyue Zhai, Shizhong Geng, Zhiming Pan and Xinan Jiao.
Title Amino acids 89–96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response.
Journal Cellular & Molecular Immunology. 2014.
Authors Lei Zhang, Zhiming Pan, Xilong Kang, Yun Yang, Heekap Kang, Na Zhang, James M Rosati and Xinan Jiao.
Title Pulmonary CD103+ dendritic cells prime Th2 responses to inhaled allergens.
Journal Mucosal Immunology. 2011.