For rapid and efficient endotoxin removal from proteins and peptides

  • Columns bind proteins of interest while endotoxins flow through
  • Proteins are desalted
  • Reduce endotoxin levels to less than 0.01 EU/µg of protein
  • Greater than 95% protein recovery
  • Concentrate protein samples and remove detergents at the same time
  • Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20

ItemsCat. #Size
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit 22800 25 preps

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also designed for the rapid removal of endotoxins from up to 200 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. This kit efficiently reduces endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes.

Kit Specifications
Maximum Protein Input
200 μg
Maximum Column Volume Input
600 μL
Molecular Weight of Recovered Proteins No molecular weight cut-off
Final Endotoxin Levels
≤ 0.01 EU/μg protein
Protein Recovery
90-95%
% Detergent Removal 90-95%
Detergents that can be removed Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 22800 (25 preps)
Binding Buffer J 8 mL
Binding Buffer N 4 mL
Wash Solution M 50 mL
Wash Solution CIP 20 mL
Wash Solution N 30 mL
Wash Solution NIP 20 mL
Elution Buffer G 6 mL
Endotoxin Removal Solution 1.5 mL
Protein Neutralizer EF 2 mL
Mini Spin Columns 25
Collection Tubes 25
Elution Tubes (1.7 mL) 25
Product Insert 1

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