Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Detection Kits
For research use only and NOT intended for in vitro diagnostics.
Potato Spindle Tuber Viroid (PSTVd) TaqMan RT-PCR Detection Kits
Overview
- Detection kits for PSTVd
- Available in TaqMan format for analysis
The Potato Spindle Tuber Viroid (PSTVd) was the first viroid to be identified, and the natural hosts for this viroid are potatoes and tomatoes. Potato spindle tuber disease has been found in the United States, Canada, Russia and South Africa, and it is one of the most destructive diseases of potatoes. PSTVd is a small (359 nucleotide), circular, single-stranded RNA molecule closely related to the Chrysanthemum stunt viroid. The viroid attacks all varieties of potato, is known to spread rapidly and often occurs in combination with virus diseases. Symptoms of PSTVd infection range from mild to severe, with severe infections resulting in colour change in the foliage, smaller leaves, spindle like elongation, and a slower rate of sprouting.
There are 2 kits available for the detection of PSTVd:
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM38650 (100 preps) | Cat. TM38610 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
PSTVd Primer & Probe Mix | 280 μL | 280 μL |
PSTVd Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |