PSTVd TaqMan Probe/Primer and Control Set (Cat. TM38610)100 rxns
Format: RT-PCR
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
The Potato Spindle Tuber Viroid (PSTVd) was the first viroid to be identified, and the natural hosts for this viroid are potatoes and tomatoes. Potato spindle tuber disease has been found in the United States, Canada, Russia and South Africa, and it is one of the most destructive diseases of potatoes. PSTVd is a small (359 nucleotide), circular, single-stranded RNA molecule closely related to the Chrysanthemum stunt viroid. The viroid attacks all varieties of potato, is known to spread rapidly and often occurs in combination with virus diseases. Symptoms of PSTVd infection range from mild to severe, with severe infections resulting in colour change in the foliage, smaller leaves, spindle like elongation, and a slower rate of sprouting.
There are 2 kits available for the detection of PSTVd:
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PLMVd TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
PLMVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
TaqMan PCR Kit -Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.
TaqManPCR Kit-Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.