Saliva RNA Collection and Preservation Devices Dx

Cat. 53800

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Saliva RNA Collection and Preservation Devices Dx

Cat. 53800

Preserve High Quality RNA, including Viral RNA, for up to 2 Months

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro diagnostic workflows
Render samples non-infectious for safe shipping and handling
Preserved RNA is suitable for a variety of downstream molecular applications, including PCR and qPCR
Preserved RNA is stable for up to 2 months at room temperature
Inactivates viruses including SARS-CoV-2 upon mixing - Explore the Application Note
Non-invasive, user-friendly sample collection
Suitable for self-collection of saliva samples supervised by a trained individual in the collection procedure by watching the training video and reading over the collection instructions.

Intended for in vitro diagnostics use.

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Saliva RNA Collection and Preservation Devices Dx
(Cat. 53800) 50 Devices

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Product Size

Kit Specifications

You have selected: Cat. 53800

Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Up to 2 months at room temperature

Shelf Life and Handling

The Collection Device should be kept tightly sealed and stored at room temperature for up to 2 years without any reduction in kit performance (The collection due date is written on the device label).
Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable.

Supporting Data

Figure 1. Stability of RNA Preserved in Norgen's Saliva RNA Preservative at room temperature for up to 2 months. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva RNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 2 months. Saliva RNA was subsequently isolated at day 0, day 4, day 6, day 16, 1 month and 2 months from 0.25 mL of the saliva/preservative sample using the supplementary protocol associated withNorgen's Total RNA Purification Kit (Cat. 17200). For visual analysis, 7.5 µL from 50 µL elution was mixed with RNA loading dye and was run on a1x MOPS agarose gel. RNA integrity was maintained after the saliva samples werestored for up to 2 months at ambient temperatures in Norgens Saliva RNA Preservative.

Figure 2. Stability of three house keeping genes over a 2 month period. GAPDH, Human 18S rRNA and beta actin genes were monitored from saliva samples stored at ambient temperature in Norgen's Saliva RNA Preservative. RNA was isolated from 250 µL of preserved samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 usingNorgen's Total RNA PurificationKit (Cat. 17200). Next, 4 µL of elution was used in 20 µL of the real-time PCR reaction (SYBR Green I) using CFX96 Touch^TM Real-Time PCR Detection System (Bio-Rad). All of the genes were preserved and stable over 2 month period.

Figure 3. Norgens Saliva RNA Preservative is able to successfully preserve saliva RNA samples when used outside of the ideal range of 1:1 at room temperature for 3 days. As the amount of saliva added to 2 mL of Saliva RNA Preservative increased from 0.2 mL to 3 mL, RNase protein and β-actin gene were monitored for RNA stability using one-step RT-PCR. The results indicated that the variation of the saliva input volumes did not significantly influence the RNA gene detection.

Figure 4. Detection of Viral, Bacterial and Human RNA from Preserved Saliva Samples. Saliva (2 mL) collected from a single healthy donor was mixed with 2 mL of Saliva RNA Preservative. Norgens COVID-19 positive control from the 2019-nCoV TaqMan RT-PCR Kit (E/RdRP gene) (Cat. TM67200) was spiked into the preserved saliva along with HeLa cells (1.6 m cells /ml ) to generate a contrived sample. From the contrived sample, total RNA was purified using Norgens Total RNA Purification Kit (Cat. 17200) and 5 genes (16s rRNA, COVID-19 E gene, RdRP-P1 and P2 gene and RP) were analyzed by PCR or RT-PCR to detect viral, bacterial and human genes.

Click for expanded view

Protocols:

(53800) Saliva RNA Collection and Preservation Devices Dx - Protocol (50 devices)

Supplementary Protocols:

Supplementary Protocol - Purification of Viral RNA with the Maxwell® RSC Viral Total Nucleic Acid Purification Kit

Application Notes:

Viral Inactivation Using Norgen’s Total Nucleic Acid Preservative and Norgen’s Saliva RNA Preservative

Inactivation of Coronavirus in Samples using Norgen's Saliva RNA Preservative

Posters:

Saliva Donor Flowchart

Product Flyers:

Customize Your Product

Title	Pre-analytical sample stabilization by different sampling devices for PCR-based COVID-19 diagnostics
Journal	New Biotechnology.2022.
Authors	Melina Hardt, Esther Föderl-Höbenreich, Stephanie Freydl, Antonio Kouros, Martina Loibner, Kurt Zatloukal

Title	Matrix Metalloproteinases and Their Tissue Inhibitors in the Pathogenesis of Periodontal Diseases in Type 1 Diabetes Mellitus
Journal	Dentistry Clinical Research.2019.
Authors	Domenyuk, D. A., Fuad Samedov, S. V. Dmitrienko, O. I. Anfinogenova, T. N. Glizhova, Diana Lysan, and Christina Nuzhnaya..

Title	Peculiarities of Phosphorine Calcium Exchange in the Pathogenesis of Dental Caries in Children with Diabetes of the First Type
Journal	Entomology and Applied Science Letters.2018.
Authors	Domenyuk, D. A., Zelensky, V. A., Dmitrienko, S. V., Anfinogenova, O. I., and Pushkin, S. V.

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Saliva RNA Collection and Preservation Devices Dx

Norgen’s Saliva RNA Collection and Preservation Devices Dx are designed for 1) safe and non-invasive supervised self-collection of saliva specimens and 2) preservation of RNA in saliva specimens at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices Dx consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen’s Saliva RNA Preservative contained within a sealed squeezable ampoule. Saliva collection is performed through supervised self-collection. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits Dx (Cat# Dx69100, Dx69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for in vitro diagnostic use for medical purposes. This kit can be used to collect and preserve viral RNA, including SARs-CoV-2 RNA for detection of COVID-19.

Norgen’s Saliva RNA Collection and Preservation Devices Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate these devices in conjunction with a downstream in vitro diagnostic assay.

SALIVA RNA PRESERVATIVE

Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh saliva specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature. The Saliva RNA Preservative also inactivates all bacteria, yeast and viruses present in the saliva sample.

NUCLEIC ACID ISOLATION FROM PRESERVATIVE

Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits Dx (Cat# Dx69100, Dx69300). To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Saliva RNA Collection Training Video | Swab Free Saliva Collection

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 53800

Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Up to 2 months at room temperature

Shelf Life and Handling

The Collection Device should be kept tightly sealed and stored at room temperature for up to 2 years without any reduction in kit performance (The collection due date is written on the device label).
Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable.

Protocols:

(53800) Saliva RNA Collection and Preservation Devices Dx - Protocol (50 devices)

Supplementary Protocols:

Supplementary Protocol - Purification of Viral RNA with the Maxwell® RSC Viral Total Nucleic Acid Purification Kit

Application Notes:

Viral Inactivation Using Norgen’s Total Nucleic Acid Preservative and Norgen’s Saliva RNA Preservative

Inactivation of Coronavirus in Samples using Norgen's Saliva RNA Preservative

Posters:

Saliva Donor Flowchart

Product Flyers:

Customize Your Product

Title	Pre-analytical sample stabilization by different sampling devices for PCR-based COVID-19 diagnostics
Journal	New Biotechnology.2022.
Authors	Melina Hardt, Esther Föderl-Höbenreich, Stephanie Freydl, Antonio Kouros, Martina Loibner, Kurt Zatloukal

Title	Matrix Metalloproteinases and Their Tissue Inhibitors in the Pathogenesis of Periodontal Diseases in Type 1 Diabetes Mellitus
Journal	Dentistry Clinical Research.2019.
Authors	Domenyuk, D. A., Fuad Samedov, S. V. Dmitrienko, O. I. Anfinogenova, T. N. Glizhova, Diana Lysan, and Christina Nuzhnaya..

Title	Peculiarities of Phosphorine Calcium Exchange in the Pathogenesis of Dental Caries in Children with Diabetes of the First Type
Journal	Entomology and Applied Science Letters.2018.
Authors	Domenyuk, D. A., Zelensky, V. A., Dmitrienko, S. V., Anfinogenova, O. I., and Pushkin, S. V.

Improve Your Workflow with Best-in-Class Total RNA Purification Technology | Learn More

Saliva RNA Collection and Preservation Devices Dx (Cat. 53800)

Saliva RNA Collection and Preservation Devices Dx

Saliva RNA Collection and Preservation Devices Dx

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Saliva RNA Collection and Preservation Devices Dx (Cat. 53800)

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