|Small RNA Library Prep Kit for Illumina||63610||6 preps|
|Small RNA Library Prep Kit for Illumina (Indexes 1-24)||63600||24 preps|
|Small RNA Library Prep Kit for Illumina (Indexes 25-48)||63620||24 preps|
Small RNA Library Prep Kit for Illumina
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.
|Number of preps||
6 or 24
|Number of indices provided||6 (for 6 preps) or 12 (for 24 preps, indexes 1-24 or 25-48)|
|Time required to complete 6 libraries||As little as 5 hours|
|Input RNA required||As little as 50 pg|
Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.
|Title||Diversity and signature of small RNA in different bodily fluids using next generation sequencing|
|Journal||BMC genomics, 19(1), 408. 2018.|
|Authors||El-Mogy, M., Lam, B., Haj-Ahmad, T. A., McGowan, S., Yu, D., Nosal, L., ... & Haj-Ahmad, Y.|
|Title||Comprehensive Profiling of the Circulatory miRNAome Response to a High Protein Diet in Elderly Men: A Potential Role in Inflammatory Response Modulation.|
|Journal||Mol Nutr Food Res. 2019 Apr;63(8):e1800811.|
|Authors||Ramzan F, Mitchell CJ, Milan AM, Schierding W, Zeng N, Sharma P, Mitchell SM, D'Souza RF, Knowles SO, Roy NC, Sjödin A, Wagner KH, Cameron-Smith D.|
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