• Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA
  • Simple and quick workflow: library could be prepared in less than 5 hours
  • No gel purification for selected types of samples
  • Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)
  • Complements Norgen's Best-in-Class Total RNA (including microRNA) Purification Technology

Related Services


NGS Service

ItemsCat. #Size
Small RNA Library Prep Kit for Illumina 63610 6 preps
Small RNA Library Prep Kit for Illumina (Indexes 1-24) 63600 24 preps
Small RNA Library Prep Kit for Illumina (Indexes 25-48) 63620 24 preps

Small RNA Library Prep Kit for Illumina

The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.

Workflow

Kit Specifications
Number of preps
6 or 24
Number of indices provided 6 (for 6 preps) or 12 (for 24 preps, indexes 1-24 or 25-48)
Time required to complete 6 libraries As little as 5 hours
Input RNA required As little as 50 pg

Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.

                                                                                                                                                      
TitleDiversity and signature of small RNA in different bodily fluids using next generation sequencing
JournalBMC genomics, 19(1), 408. 2018.
AuthorsEl-Mogy, M., Lam, B., Haj-Ahmad, T. A., McGowan, S., Yu, D., Nosal, L., ... & Haj-Ahmad, Y.
                                                                                                                                                      
TitleComprehensive Profiling of the Circulatory miRNAome Response to a High Protein Diet in Elderly Men: A Potential Role in Inflammatory Response Modulation.
JournalMol Nutr Food Res. 2019 Apr;63(8):e1800811.
AuthorsRamzan F, Mitchell CJ, Milan AM, Schierding W, Zeng N, Sharma P, Mitchell SM, D'Souza RF, Knowles SO, Roy NC, Sjödin A, Wagner KH, Cameron-Smith D.