• Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA
  • Simple and quick workflow: library could be prepared in less than 5 hours
  • No gel purification for selected types of samples
  • Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)
  • Complements Norgen's Best-in-Class Total RNA (including microRNA) Purification Technology

ItemsCat. #Size
Small RNA Library Prep Kit for Illumina 63610 6 preps
Small RNA Library Prep Kit for Illumina 63600 24 preps

Small RNA Library Prep Kit for Illumina

The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.

Workflow

Kit Specifications
Number of preps
6 or 24
Number of indices provided 6 (for 6 preps) or 12 (for 24 preps)
Time required to complete 6 libraries As little as 5 hours
Input RNA required As little as 50 pg

Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.


Kit Components
3’ Adaptor Ligation Reagents
Component Cat. 63450 (6 preps) Cat. 64600 (24 preps)
3' Adaptor 6 µL 24 µL
3' Adaptor Ligation Master Mix 72 µL 288 µL
T4 RNA Ligase 2 (truncated) 6 µL 24 µL
Nuclease-Free Water 1 mL 1 mL
5’ Adaptor Ligation Reagents
Component Cat. 63450 (6 preps) Cat. 64600 (24 preps)
5' Adaptor 6 µL 24 µL
5' Adaptor Ligation Master Mix 66 µL 264 µL
T4 RNA Ligase 1 6 µL 24 µL
cDNA Synthesis Reagents
Component Cat. 64650 (6 preps) Cat. 64600 (24 preps)
Reverse Primer 6 µL 24 µL
cDNA Synthesis Master Mix 48 µL 192 µL
TruScript Reverse Transcriptase 6 µL 24 µL
PCR Amplification and Index Reagents
Component Cat. 63450 (6 preps) Cat. 64600 (24 preps)
2x NGS PCR Master Mix 300 µL 1.2 mL
Forward Index Primer 3 µL (6 Indices Total) 6 µL (24 Indices Total)
PCR Reverse Primer 18 µL 72 µL
NGS Reaction Clean-Up Kit
Component Cat. 63400 (18 preps) Cat. 63500 (75 preps)
Buffer RL 6 mL 40 mL
Wash Solution A

3 x 2.4 mL (Reconstituted to 8 mL)

38 mL (Reconstituted to 128 mL)
Elution Solution A 1 mL 6 mL
Columns 18 75
Gel Filtration Columns 6 24
Collection Tubes 18 75
Elution Tubes 18 75
Product Insert 1 1
Supplementary Components for Size Selection
Component Cat. 64650 (6 preps) 64600 (24 preps)
NGS MW Ladder 50 µL 50 µL
NGS Control Ladder 50 µL 50 µL
Loading Dye 300 µL 300 µL