Soil DNA Isolation 96-Well Kit Rapid, high throughput purification of DNA from soil samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil DNA Isolation 96-Well Kit Rapid, high throughput purification of DNA from soil samples Price USD$1,025.00 SKU 26560 Format High Throughput Size 2 x 96-Well Plates Quantity Overview Details Documentation Citations Overview Fast and easy high throughput processing using either a vacuum manifold or centrifugationProcess all soil types including clay, loam, sandy soils and high humic content soils such as peat, compost and manureRemove organic substances using the OSR SolutionRemove all humic acid from DNA samples using the Humic Acid Removal plateIsolate high quality total DNA from all soil types - ready for any downstream PCR, qPCRExcellent DNA for metagenomic studiesNo phenol or chloroform extractions This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation. Details Supporting Data Previous Figure 1. Consistent Yield of High Quality of Soil DNA. Total soil DNA was isolated from gardening top soil samples using Norgen's Soil DNA Isolation 96-Well Kit. For analysis, 10 µL from each 100 µL elution from 10 different wells was run on a 1.2% TAE agarose gel. Lane M is Norgen's High Ranger DNA ladder. Figure 2. Consistent and high DNA quality. Total DNA was isolated from gardening top soil using Norgen's Soil DNA Isolation 96-Well Kit. For analysis of DNA quality, 3 µL from each 100 µL elution was used as the template in a real-time PCR reaction (SYBR Green) for the detection of the 16s rDNA. All DNA template from different wells showed a consistent Ct, indicating the consistent high DNA quality and yield. Black circle: NTC. Next Click for expanded view Kit Specifications Binding Capacity Per Well 50 μg Maximum Loading Volume Per Well 500 μL Size of DNA Purified All sizes Maximum Amount of Starting Material 250 mg Time to Complete 96 Purifications 50 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 26560 (192 preps) Lysis Buffer G 2 x 100 mL Lysis Additive A 25 mL Binding Buffer I 25 mL OSR Solution 12 mL Wash Solution A 2 x 38 mL Elution Buffer B 30 mL Bead Tubes 200 HAR Plate 2 96-Well Filter Plate 2 Adhesive Tape 4 96-Well Collection Plate 4 96-Well Elution Plate 2 Product Insert 1 Documentation PROTOCOLS (26560) Soil DNA Isolation 96 Well Kit - Protocol (2 x 96-well) APPLICATION NOTES Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods SAFETY DATA SHEETS (26560) Soil DNA Isolation 96 Well Kit - Safety Data Sheet FLYERS Flyer Citations Title Ecotoxicological effects of a synthetic and a natural insecticide on earthworms and soil bacterial community Journal Environmental Advances. 2015. Authors Arianna De Bernardi, Enrica Marini, Cristiano Casucci, Luca Tiano, Fabio Marcheggiani, Maurizio Ciani, Francesca Comitini, Eren Taskin, Edoardo Puglisi, Costantino Vischetti Title Metagenomic and satellite analyses of red snow in the Russian Arctic Journal PeerJ. 2015. Authors Hisakawa N, Quistad SD, Hester ER, Martynova D, Maughan H, Sala E, Gavrilo MV, Rohwer F Related Products Plant/Fungi DNA Isolation Kit Water RNA/DNA Purification Kit - 0.45 µm
Soil DNA Isolation 96-Well Kit Rapid, high throughput purification of DNA from soil samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil DNA Isolation 96-Well Kit Rapid, high throughput purification of DNA from soil samples Price USD$1,025.00 SKU 26560 Format High Throughput Size 2 x 96-Well Plates Quantity
