For the rapid preparation of inhibitor-free DNA from all types of soil, including difficult samples with high humic acid content

  • Isolate total DNA from all microorganisms found in soil, including bacteria, fungi and algae
  • Process all types of soil, including common soil samples such as clay, loam and sandy soils and difficult samples with high humic acid such as compost, peat and manure
  • Remove all humic acids using the provided Humic Acid Removal Columns and the OSR Solution
  • Isolated DNA is free of inhibitors such as humic acid - ready for PCR applications and any metagenomic study
  • Easy- to-use beads for extraction - fast without any time consuming sample grinding

Items Cat. # Size
Soil DNA Isolation Kit 26500 50 preps

Soil DNA Isolation Kit

This kit provides a convenient and rapid method for the isolation of DNA from microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples such as clay, loam and sandy soil and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution.  Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae.  DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications, any metagenomic study and more, as all humic acid substances and PCR inhibitors are removed during the isolation.

PRODUCT DEMONSTRATION VIDEO

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Kit Specifications
Maximum Soil Input
250 mg
Type of Soil Processed
All soil types, including clay,
loam and sandy soil, compost
and manure
Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 26500 (50 preps)
Lysis Buffer G 45 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
OSR Solution 3 mL
Buffer SK 60 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tubes 50
Humic Acid Removal Columns 50
Spin Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.
Journal American Chemical Society. 2014.
Authors Shirley Y. Wong, Athanasios Paschos, Radhey S. Gupta, and Herb E. Schellhorn.
Title Network topology reveals high connectance levels and few key microbial genera within soils.
Journal Frontiers in Environmental Science. 2014.
Authors Lupatini M, Suleiman A, Jacques R, Antoniolli Z, Ferreira A, Kuramae E and Roesch L.
Title Optimization and Validation of Molecular Assays for Invasive Tunicate Monitoring in Environmental Water Samples.
Journal Macrothink Institute. 2013.
Authors Stewart-Clark S, Davidson J, Greenwood S.
Title Bioremediation of high organic load lagoon sediments: Compost addition and priming effects.
Journal Chemosphere. 2012.
Authors d'Errico G, Giovannelli D, Montano C, Milanovic V, Ciani M, Manini E.
Title Molecular Approaches to the Study of Biological Phosphorus Cycling.
Journal Soil Biology. 2010.
Authors Wasaki J. and Maruyama H.