Soil DNA Isolation Maxi Kit
For the detection of microorganisms from large soil samples
For research use only and NOT intended for in vitro diagnostics.
Soil DNA Isolation Maxi Kit
For the detection of microorganisms from large soil samples
Overview
- Rapid and convenient method to detect microorganisms in up to 10 g of soil samples
- Process all soil types
- Efficiently remove organic substances using the OSR Solution
- Remove all humic acid from DNA samples using the Maxi Humic Acid Removal Columns
- Fast and easy processing using a rapid spin-column format
- Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae
Norgen’s Soil DNA Isolation Maxi Kit provides a convenient and rapid method for the detection of microorganisms from large soil samples (up to 10 grams). All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and NGS applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Details
Supporting Data
Kit Specifications
|
|
Maximum Soil Input |
10 g
|
Type of Soil Processed |
All soil types
|
Maximum Column Binding Capacity |
1.5 mg
|
Average Yield from 10 g of soil* |
30-50 μg
|
Time to Complete 10 Purifications |
35 minutes (1 hour with OSR steps)
|
* Average yields will vary depending upon soil sample types
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 62000 (10 preps) |
---|---|
Lysis Buffer G | 2 x 100 mL |
Lysis Additive A | 25 mL |
Binding Buffer I | 25 mL |
OSR Solution | 12 mL |
Buffer SK | 2 x 60 mL |
Wash Solution A | 1 x 18 mL 3 x 38 mL |
Elution Buffer B | 30 mL |
Maxi Bead Tubes | 10 |
Humic Acid Removal Columns | 10 |
Maxi Spin Columns | 10 |
Elution Tubes | 10 |
Product Insert | 1 |