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Soil DNA Isolation Maxi Kit
Rapid and convenient method to detect microorganisms in up to 10 g of soil samples
Process all soil types
Efficiently remove organic substances using the OSR Solution
Remove all humic acid from DNA samples using the Maxi Humic Acid Removal Columns
Fast and easy processing using a rapid spin-column format
Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae
Norgen’s Soil DNA Isolation Maxi Kit provides a convenient and rapid method for the detection of microorganisms from large soil samples (up to 10 grams). All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and NGS applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Figure 1. Resolution of DNA isolated from 10 g of two different soil types; regular soil (clay) and high humic acid soil (top soil) using Norgens Soil DNA Isolation Maxi Kit. For evaluation, 10 µL from the 1st elution (2 mL) and 2nd elution (2 mL) from each soil sample was run on 1X TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Soil DNA Isolation Maxi Kit. Marker = Norgens HighRanger DNA Ladder (Cat. 11900)
Figure 2. Difference in DNA yield between two soil types (clay and top soil) and elutions (1st and 2nd elution). Ten grams of soil was processed using Norgens Soil DNA Isolation Maxi Kit. The second elution increased the total DNA yield by an additional 20-30%.
Figure 3. High soil DNA quality was confirmed by real-time PCR using 8 µL of soil DNA (total PCR reaction volume was 20 µL) isolated using Norgens Soil DNA Isolation Maxi Kit to detect 16s rDNA. The 16s rRNA gene was successfully amplified in both the first elution (red) and second elution (blue) from two soil types (Clay = circle, Top soil = diamond) without PCR inhibition, indicating the excellent soil DNA quality from 10 grams of soil.