Soil DNA Isolation Maxi Kit For the detection of microorganisms from large soil samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil DNA Isolation Maxi Kit For the detection of microorganisms from large soil samples Price USD$275.00 SKU 62000 Format Maxi Size 10 Preps Quantity Overview Details Documentation Citations Overview Rapid and convenient method to detect microorganisms in up to 10 g of soil samples Process all soil types Efficiently remove organic substances using the OSR Solution Remove all humic acid from DNA samples using the Maxi Humic Acid Removal Columns Fast and easy processing using a rapid spin-column format Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae Norgen’s Soil DNA Isolation Maxi Kit provides a convenient and rapid method for the detection of microorganisms from large soil samples (up to 10 grams). All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and NGS applications, as all humic acid substances and PCR inhibitors are removed during the isolation. Details Supporting Data Previous Figure 1. Resolution of DNA isolated from 10 g of two different soil types; regular soil (clay) and high humic acid soil (top soil) using Norgen’s Soil DNA Isolation Maxi Kit. For evaluation, 10 µL from the 1st elution (2 mL) and 2nd elution (2 mL) from each soil sample was run on 1X TAE 1.2% agarose gel. Excellent DNA integrity and yield were observed from the Soil DNA Isolation Maxi Kit. Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900) Figure 2. Difference in DNA yield between two soil types (clay and top soil) and elutions (1st and 2nd elution). Ten grams of soil was processed using Norgen’s Soil DNA Isolation Maxi Kit. The second elution increased the total DNA yield by an additional 20-30%. Figure 3. High soil DNA quality was confirmed by real-time PCR using 8 µL of soil DNA (total PCR reaction volume was 20 µL) isolated using Norgen’s Soil DNA Isolation Maxi Kit to detect 16s rDNA. The 16s rRNA gene was successfully amplified in both the first elution (red) and second elution (blue) from two soil types (Clay = circle, Top soil = diamond) without PCR inhibition, indicating the excellent soil DNA quality from 10 grams of soil. Next Click for expanded view Kit Specifications Maximum Soil Input 10 g Type of Soil Processed All soil types Maximum Column Binding Capacity 1.5 mg Average Yield from 10 g of soil* 30-50 μg Time to Complete 10 Purifications 35 minutes (1 hour with OSR steps) * Average yields will vary depending upon soil sample types Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 62000 (10 preps) Lysis Buffer G 2 x 100 mL Lysis Additive A 25 mL Binding Buffer I 25 mL OSR Solution 12 mL Buffer SK 2 x 60 mL Wash Solution A 1 x 18 mL 3 x 38 mL Elution Buffer B 30 mL Maxi Bead Tubes 10 Humic Acid Removal Columns 10 Maxi Spin Columns 10 Elution Tubes 10 Product Insert 1 Documentation PROTOCOLS PI62000-2 Soil DNA Maxi Kit Insert.pdf APPLICATION NOTES App-Note-82-DNA-Molecular-Weight.pdf SAFETY DATA SHEETS 62000_SDS.pdf FLYERS Soil DNA Maxi v1.pdf Citations Powered by Bioz See more details on Bioz Related Products Soil DNA Isolation Kit Water RNA/DNA Purification Kit - 0.22 µm HighRanger 1 kb DNA Ladder UltraRanger 1 kb DNA Ladder
Soil DNA Isolation Maxi Kit For the detection of microorganisms from large soil samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Soil DNA Isolation Maxi Kit For the detection of microorganisms from large soil samples Price USD$275.00 SKU 62000 Format Maxi Size 10 Preps Quantity