• Rapid and convenient method to detect microorganisms in soil samples
  • Process all soil types
  • Remove organic substances using the OSR Solution
  • Remove all humic acid from DNA samples
  • Fast and easy processing using a rapid spin-column format
  • Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae

ItemsCat. #Size
Soil DNA Isolation Plus Kit 64000 50 preps
Soil DNA Isolation Plus Kit 64060 100 preps

Soil DNA Isolation Plus Kit

Norgen's Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

Kit Specifications
Maximum Soil Input
250 mg
Type of Soil Processed
All soil types
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 64000 (50 preps)
Lysis Buffer G 45 mL
Lysis Additive A 2 x 6 mL
Binding Buffer I 7 mL
OSR Solution 3 mL
Lysis Buffer QP 25 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Evaluating the production and bio-stimulating effect of 5-methyl 1, hydroxy phenazine on microbial fuel cell performance
Journal International Journal of Environmental Science and Technology. 2017.
Authors S. Yousaf, M. Anam, N. Ali
Title Comparison of Total DNA Extraction Methods for Microbial Community from Polluted Soil
Journal Conference paper. 2015.
Authors Ana-Maria Tanase, Ioana Mereuta Iulia Chiciudean, Robertina Ionescu, Ligia Milea, Călina Petruța Cornea, Tatiana Vassu, Ileana Stoica
Title Microbial community distribution and genetic analysis in a sludge active treatment for a complex industrial wastewater: a study using microbiological and molecular analysis and principal component analysis
Journal Annals of Microbiology. 2015.
Authors Giulio Moretti, Federica Matteucci, Claudia Ercole, Francesco Vegliò, Maddalena Del Gallo
Title Molecular Characterization and Potential of Bacterial Species Associated with Cassava Waste
Journal Nigerian Food Journal. 2014.
Authors A.I. Elijah, , O.O. Atanda, A.R. Popoola, S.V.A. Uzochukwu
Title Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments.
Journal American Chemical Society. 2014.
Authors Shirley Y. Wong, Athanasios Paschos, Radhey S. Gupta, and Herb E. Schellhorn.
Title Network topology reveals high connectance levels and few key microbial genera within soils.
Journal Frontiers in Environmental Science. 2014.
Authors Lupatini M, Suleiman A, Jacques R, Antoniolli Z, Ferreira A, Kuramae E and Roesch L.
Title Optimization and Validation of Molecular Assays for Invasive Tunicate Monitoring in Environmental Water Samples.
Journal Macrothink Institute. 2013.
Authors Stewart-Clark S, Davidson J, Greenwood S.
Title Bioremediation of high organic load lagoon sediments: Compost addition and priming effects.
Journal Chemosphere. 2012.
Authors d'Errico G, Giovannelli D, Montano C, Milanovic V, Ciani M, Manini E.
Title Molecular Approaches to the Study of Biological Phosphorus Cycling.
Journal Soil Biology. 2010.
Authors Wasaki J. and Maruyama H.