Soil DNA Isolation Plus Kit
For the detection of microorganisms from soil samples
For research use only and NOT intended for in vitro diagnostics.
Soil DNA Isolation Plus Kit
For the detection of microorganisms from soil samples
Overview
- Rapid and convenient method to detect microorganisms in soil samples
- Process all soil types
- Remove organic substances using the OSR Solution
- Remove all humic acid from DNA samples
- Fast and easy processing using a rapid spin-column format
- Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae
Norgen's Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Details
Supporting Data
Kit Specifications
|
|
Maximum Soil Input |
250 mg
|
Type of Soil Processed |
All soil types
|
Maximum Column Binding Capacity |
50 μg
|
Maximum Column Loading Volume |
650 μL
|
Time to Complete 10 Purifications |
30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment.
Component | Cat. 64000 (50 preps) |
---|---|
Lysis Buffer G | 45 mL |
Lysis Additive A | 2 x 6 mL |
Binding Buffer I | 7 mL |
OSR Solution | 3 mL |
Lysis Buffer QP | 25 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 8 mL |
Bead Tubes | 50 |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Documentation
Citations
Title | Enhancing bioelectrochemical hydrogen production from industrial wastewater using Ni-foam cathodes in a microbial electrolysis cell pilot plant |
Journal | Water Research. 2024. |
Authors | Oscar Guerrero-Sodric, Juan Antonio Baeza, Albert Guisasola |
Title | Coupling anammox and heterotrophic denitrification activity at mainstream conditions in a single reactor unit |
Journal | Chemical Engineering Journal. 2022. |
Authors | Xènia Juan-Díaz, Lluc Olmo, Julio Pérez, Julián Carrera |
Title | Comparative Metagenomics of Anaerobic Digester Communities Reveals Sulfidogenic and Methanogenic Microbial Subgroups in Conventional and Plug Flow Residential Septic Tank Systems |
Journal | Processes. 2022. |
Authors | James Naphtali, Alexander W. Y. Chan, Faizan Saleem, Enze Li, Jacob Devries and Herb E. Schellhorn |
Title | Ceramic-supported graphene oxide membrane bioreactor for the anaerobic decolorization of azo dyes |
Journal | Journal of Water Process Engineering. 2022. |
Authors | Mohammad Shaiful Alam Amin, Frank Stüber, Jaume Giralt, Agustí Fortuny, Azael Fabregat, Josep Font |
Title | Evaluating the production and bio-stimulating effect of 5-methyl 1, hydroxy phenazine on microbial fuel cell performance |
Journal | International Journal of Environmental Science and Technology. 2017. |
Authors | S. Yousaf, M. Anam, N. Ali |
Title | Comparison of Total DNA Extraction Methods for Microbial Community from Polluted Soil |
Journal | Agriculture and Agricultural Science Procedia. 2015. |
Authors | Ana-Maria Tanase, Ioana Mereuta Iulia Chiciudean, Robertina Ionescu, Ligia Milea, Călina Petruța Cornea, Tatiana Vassu, Ileana Stoica |
Title | Microbial community distribution and genetic analysis in a sludge active treatment for a complex industrial wastewater: a study using microbiological and molecular analysis and principal component analysis |
Journal | Annals of Microbiology. 2015. |
Authors | Giulio Moretti, Federica Matteucci, Claudia Ercole, Francesco Vegliò, Maddalena Del Gallo |
Title | It takes an individual plant to raise a community: TRFLP analysis of the rhizosphere microbial community of two pairs of high- and low-metal-accumulating plants. |
Journal | Soil Biology and Biochemistry. 2015. |
Authors | Melanie P. Columbus, Sheila M. Macfie. |
Title | Molecular Characterization and Potential of Bacterial Species Associated with Cassava Waste |
Journal | Nigerian Food Journal. 2014. |
Authors | A.I. Elijah, , O.O. Atanda, A.R. Popoola, S.V.A. Uzochukwu |
Title | Insertion/Deletion-Based Approach for the Detection of Escherichia coli O157:H7 in Freshwater Environments. |
Journal | American Chemical Society. 2014. |
Authors | Shirley Y. Wong, Athanasios Paschos, Radhey S. Gupta, and Herb E. Schellhorn. |
Title | Network topology reveals high connectance levels and few key microbial genera within soils. |
Journal | Frontiers in Environmental Science. 2014. |
Authors | Lupatini M, Suleiman A, Jacques R, Antoniolli Z, Ferreira A, Kuramae E and Roesch L. |
Title | Optimization and Validation of Molecular Assays for Invasive Tunicate Monitoring in Environmental Water Samples. |
Journal | Macrothink Institute. 2013. |
Authors | Stewart-Clark S, Davidson J, Greenwood S. |
Title | Bioremediation of high organic load lagoon sediments: Compost addition and priming effects. |
Journal | Chemosphere. 2012. |
Authors | d'Errico G, Giovannelli D, Montano C, Milanovic V, Ciani M, Manini E. |
Title | Molecular Approaches to the Study of Biological Phosphorus Cycling. |
Journal | Soil Biology. 2010. |
Authors | Wasaki J. and Maruyama H. |