Figure 2. Detection of Bacterial RNA from Stool Samples. Real-time one step RT-PCR analysis (SYBR Green) was carried out to detect the bacterial 16s rRNA expression from six stool samples isolated using Norgen's Stool Nucleic Acid Isolation Kit. The RNA template was treated with DNase I and concentrated with Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600, 43200). No PCR inhibition was observed from 300 ng of total RNA in 20 µL PCR reaction. Black diamond: negative control, Blue diamond: six stool RNA samples.

Figure 3. Isolation of Genomic DNA from Human Stool Samples. Total RNA and DNA was isolated from 6 different 200 mg human stool samples using Norgen's Stool Nucleic Acid Isolation Kit. For analysis, 10 µL of each 75 µL elution was run on a 1.2% 1xTAE agarose gel. High quality DNA was isolated from all six stool samples. M: Norgen's HighRanger 1kb DNA ladder (Cat. 11900).

Figure 4. Detection of Human DNA from Stool Samples. Total RNA and DNA were isolated from two different stool samples using Norgen's Stool Nucleic Acid Isolation Kit. Two microliters of each elution was used in 20 µL of Norgen's 2X PCR Mastermix spiked with SYBR green (Bio-Rad), using 0.3 µM of primers against GAPDH. As can be seen in the amplification plot, all four samples were successfully amplified, indicating that PCR inhibitors were removed and the kit isolated high quality of DNA from stool. PCR quantification data (A) and melting curve analysis date (B) are shown.