Stool Nucleic Acid Isolation Kit

Cat. 45600
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Stool Nucleic Acid Isolation Kit

Cat. 45600

A convenient and rapid method to isolate total DNA and RNA from fresh, frozen and preserved stool samples.

  • Simultaneous isolation of both host and microbial DNA and RNA
  • Eliminates PCR inhibitors including humic acids
  • High quality total RNA and DNA for sensitive downstream applications
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Stool Nucleic Acid Isolation Kit
(Cat. 45600)
50 preps

Kit Size

  • Simultaneous isolation of both host and microbial DNA and RNA
  • Eliminates PCR inhibitors including humic acids
  • High quality total RNA and DNA for sensitive downstream applications
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Stool Nucleic Acid Isolation Kit

This kit provides a rapid spin column method to isolate total DNA and RNA from fresh or frozen stool samples, as well as from stool collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes. The universal protocol conveniently allows for the isolation of total genomic DNA and total RNA from all the various microorganisms and host cells found in the stool sample simultaneously.  The kit removes all traces of humic acids and provides consistent, high yields of RNA and DNA.

The purified DNA and RNA are of the highest quality and are fully compatible with downstream PCR applications, as all humic acid substances and other PCR inhibitors are removed during the isolation.

Kit Specifications

You have selected: Cat. 45600
Kit Specifications
Maximum Stool Input
200 mg
Type of Stool Processed
Preserved, frozen, and fresh stool
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 45600 (50 preps)
Lysis Buffer C 60 mL
Binding Buffer E 6 mL
Wash Solution A 38 mL
Elution Buffer E 6 mL
Bead Tube 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects
Journal Microorganisms. 2022.
Authors Alessandra De Giani, Anna Sandionigi, Jessica Zampolli, Angela Michelotti, Francesco Tursi, Massimo Labra and Patrizia Di Gennaro
Title Effectiveness of Multistrain Probiotic Formulation on Common Infectious Disease Symptoms and Gut Microbiota Modulation in Flu-Vaccinated Healthy Elderly Subjects
Journal BioMed Research International. 2022.
Authors Anna Sandionigi, Alessandra De Giani, Francesco Tursi, Angela Michelotti, Enza Cestone, Silvana Giardina, Jessica Zampolli, and Patrizia Di Gennaro
Title Escherichia Coli as a Marker for Hypertriglyceridemia
Journal United States Patent Application 20160168627. 2016.
Authors Chou CJ, Darimont-nicolau C, Membrez M

Stool Nucleic Acid Isolation Kit

This kit provides a rapid spin column method to isolate total DNA and RNA from fresh or frozen stool samples, as well as from stool collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes. The universal protocol conveniently allows for the isolation of total genomic DNA and total RNA from all the various microorganisms and host cells found in the stool sample simultaneously.  The kit removes all traces of humic acids and provides consistent, high yields of RNA and DNA.

The purified DNA and RNA are of the highest quality and are fully compatible with downstream PCR applications, as all humic acid substances and other PCR inhibitors are removed during the isolation.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 45600
Kit Specifications
Maximum Stool Input
200 mg
Type of Stool Processed
Preserved, frozen, and fresh stool
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component Cat. 45600 (50 preps)
Lysis Buffer C 60 mL
Binding Buffer E 6 mL
Wash Solution A 38 mL
Elution Buffer E 6 mL
Bead Tube 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Effects of Inulin-Based Prebiotics Alone or in Combination with Probiotics on Human Gut Microbiota and Markers of Immune System: A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Subjects
Journal Microorganisms. 2022.
Authors Alessandra De Giani, Anna Sandionigi, Jessica Zampolli, Angela Michelotti, Francesco Tursi, Massimo Labra and Patrizia Di Gennaro
Title Effectiveness of Multistrain Probiotic Formulation on Common Infectious Disease Symptoms and Gut Microbiota Modulation in Flu-Vaccinated Healthy Elderly Subjects
Journal BioMed Research International. 2022.
Authors Anna Sandionigi, Alessandra De Giani, Francesco Tursi, Angela Michelotti, Enza Cestone, Silvana Giardina, Jessica Zampolli, and Patrizia Di Gennaro
Title Escherichia Coli as a Marker for Hypertriglyceridemia
Journal United States Patent Application 20160168627. 2016.
Authors Chou CJ, Darimont-nicolau C, Membrez M

 


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