Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
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Stool Nucleic Acid Isolation Kit
Simultaneous isolation of both host and microbial DNA and RNA
Eliminates PCR inhibitors including humic acids
High quality total RNA and DNA for sensitive downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid spin column method to isolate total DNA and RNA from fresh or frozen stool samples, as well as from stool collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes. The universal protocol conveniently allows for the isolation of total genomic DNA and total RNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and provides consistent, high yields of RNA and DNA.
The purified DNA and RNA are of the highest quality and are fully compatible with downstream PCR applications, as all humic acid substances and other PCR inhibitors are removed during the isolation.
Figure 1. Total RNA Profile from Different Stool Samples. Total RNA and DNA were isolated from 6 different 200 mg human stool samples using Norgen's Stool Nucleic Acid Isolation Kit. For analysis, 7.5 µL from each 75 µL elution were loaded on 1.2 % MOPS agrarose gel. All six sample showed a good RNA integrity and total RNA profile that includes large and small RNA.
Figure 2. Detection of Bacterial RNA from Stool Samples. Real-time one step RT-PCR analysis (SYBR Green) was carried out to detect the bacterial 16s rRNA expression from six stool samples isolated using Norgen's Stool Nucleic Acid Isolation Kit. The RNA template was treated with DNase I and concentrated with Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600, 43200). No PCR inhibition was observed from 300 ng of total RNA in 20 µL PCR reaction. Black diamond: negative control, Blue diamond: six stool RNA samples.
Figure 3. Isolation of Genomic DNA from Human Stool Samples. Total RNA and DNA was isolated from 6 different 200 mg human stool samples using Norgen's Stool Nucleic Acid Isolation Kit. For analysis, 10 µL of each 75 µL elution was run on a 1.2% 1xTAE agarose gel. High quality DNA was isolated from all six stool samples. M: Norgen's HighRanger 1kb DNA ladder (Cat. 11900).
Figure 4. Detection of Human DNA from Stool Samples. Total RNA and DNA were isolated from two different stool samples using Norgen's Stool Nucleic Acid Isolation Kit. Two microliters of each elution was used in 20 µL of Norgen's 2X PCR Mastermix spiked with SYBR green (Bio-Rad), using 0.3 µM of primers against GAPDH. As can be seen in the amplification plot, all four samples were successfully amplified, indicating that PCR inhibitors were removed and the kit isolated high quality of DNA from stool. PCR quantification data (A) and melting curve analysis date (B) are shown.