Synovial Fluid Bacterial Genomic DNA Purification Kit

Cat. 67900
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Synovial Fluid Bacterial Genomic DNA Purification Kit

Cat. 67900

For isolating the highest quality and quantity of bacterial genomic DNA from various amounts of synovial fluid

  • Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
  • Rapid and convenient spin column protocol
  • Purified bacterial gDNA has a minimal host gDNA contamination.
  • High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more

For research use only and NOT intended for in vitro diagnostics.

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Synovial Fluid Bacterial Genomic DNA Isolation Kit
(Cat. 67900)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 67900
Kit Specifications
Maximum Input
2 x 109 bacterial cells
Column Binding Capacity
25 μg
Average Yield
up to 20 µg
Time to Complete 10 Purifications
45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Supporting Data

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Component Cat. 67900 (50 preps)
Precipitation Solution B 15 mL
Lysis Buffer T 6.5 mL
Solution BX 28 mL
Wash Solution A 38 mL
Elution Buffer B 8 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Synovial Fluid Bacterial Genomic DNA Purification Kit

Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.  

Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL.  Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 67900
Kit Specifications
Maximum Input
2 x 109 bacterial cells
Column Binding Capacity
25 μg
Average Yield
up to 20 µg
Time to Complete 10 Purifications
45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Component Cat. 67900 (50 preps)
Precipitation Solution B 15 mL
Lysis Buffer T 6.5 mL
Solution BX 28 mL
Wash Solution A 38 mL
Elution Buffer B 8 mL
Micro Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

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