Synovial Fluid Bacterial Genomic DNA Purification Kit For isolating the highest quality and quantity of bacterial genomic DNA from various amounts of synovial fluid For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Synovial Fluid Bacterial Genomic DNA Purification Kit For isolating the highest quality and quantity of bacterial genomic DNA from various amounts of synovial fluid Price USD$339.00 SKU 67900 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative) Rapid and convenient spin column protocol Purified bacterial gDNA has a minimal host gDNA contamination. High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests. Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS. Details Supporting Data Previous Figure 1. Isolation and Detection of the Gram +Ve S. Aureus Bacterial Genomic DNA from 1 mL of spike-in Synovial fluid. Genomic DNA was isolated from 1 mL synovial fluid spiked with a serially diluted Gram +Ve S. Aureus using Norgen’s Synovial Fluid Bacterial genomic DNA Purification Kit. The efficiency of the purified bacterial gDNA from the spiked-in synovial fluid was evaluated against the gDNA isolation from pure culture containing the same amount of S. Aureus spiked in 1mL synovial fluid. The purified gDNA was subsequently detected using quantitative PCR. All serially diluted spiked-in Gram +Ve S. Aureus was purified with high efficiency from 1mL synovial fluid as compared to the amplification of S. Aureus from pure culture. The limit of detection for the Gram +Ve S. Aureus was down to 5 CFU/mL. Figure 2. Isolation and Detection of the Gram -Ve E.coli Bacterial Genomic DNA from 1 mL of spike-in Synovial fluid. Genomic DNA was isolated from 1 mL synovial fluid spiked with a serially diluted Gram -Ve E.coli using Norgen’s Synovial Fluid Bacterial genomic DNA Purification Kit. The efficiency of the purified bacterial gDNA from the spiked-in synovial fluid was evaluated against the gDNA isolation from pure culture containing the same amount of E.coli spiked in 1mL synovial fluid. The purified gDNA was subsequently detected using quantitative PCR. All serially diluted spiked-in Gram -Ve E.coli was purified with high efficiency from 1mL synovial fluid as compared to the amplification of E.coli from pure culture. The limit of detection for the Gram -Ve E.coli was down to 5 CFU/mL. Next Click for expanded view Kit Specifications Maximum Input 2 x 109 bacterial cells Column Binding Capacity 25 μg Average Yield up to 20 µg Time to Complete 10 Purifications 45 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. Component Cat. 67900 (50 preps) Precipitation Solution B 15 mL Lysis Buffer T 6.5 mL Solution BX 28 mL Wash Solution A 38 mL Elution Buffer B 8 mL Micro Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (67900) Synovial Fluid Bacterial Genomic DNA Isolation Kit - Protocol
Synovial Fluid Bacterial Genomic DNA Purification Kit For isolating the highest quality and quantity of bacterial genomic DNA from various amounts of synovial fluid For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Synovial Fluid Bacterial Genomic DNA Purification Kit For isolating the highest quality and quantity of bacterial genomic DNA from various amounts of synovial fluid Price USD$339.00 SKU 67900 Format Spin Column Size 50 Preps Quantity