Ready-to-Use TaqMan Master Mixes with pre-mixed internal PCR control (HEX/VIC)

  • Use with Norgen's TaqMan Probe/Primer and Control Sets
  • For any PCR application where the input template quality is important to avoid false negatives
  • Convenience and time savings
  • Cost efficient
  • High sensitivity

ItemsCat. #Size
TaqMan 2x PCR Master Mix 28340 100 rxns
TaqMan 2x RT- PCR Master Mix 28341 100 rxns

TaqMan 2x PCR Master Mix & TaqMan 2x RT-PCR Master Mix

 

 

Description 
Norgen’s TaqMan 2x PCR Master Mix is a ready-to-use TaqMan 2x PCR Master Mix solution that contains a PCR internal control which can be detected by HEX/VIC channel in a real-time PCR machine. By detecting the internal control users can validate the DNA template quality, thereby preventing any false negatives in the PCR results. The user needs only to add template, target TaqMan primer/probe mix and water to set up the TaqMan real-time PCR. 

 

PCR Control: 
TaqMan 2x PCR Master Mix contains PCR control primers/probe (HEX/VIC) and PCR control template. The PCR control reaction in the TaqMan 2x PCR Master Mix is optimized to not interfere with target amplification. The fluorescence of the target probe should not be HEX/VIC.

 

 

Description 
Norgen’s TaqMan 2x RT-PCR Master Mix is a ready-to-use solution that contains a PCR internal control which can be detected by HEX/VIC channel in a real-time PCR machine. By detecting the internal control users can validate the RNA template quality, thereby preventing any false negatives in the RT-PCR results. The user needs only to add template, target TaqMan primer/probe mix and water to set up the TaqMan real-time RT-PCR.

 

PCR Control:

TaqMan 2x RT-PCR Master Mix contains PCR control primers/probe (HEX/VIC) and PCR control template. The PCR control reaction in the TaqMan 2x RT-PCR Master Mix is optimized to not interfere with target amplification. The fluorescence of the target probe should not be HEX/VIC.

 

Reagents Supplied – Cat # 28340

  • TaqMan 2x PCR Master Mix (3 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents Supplied – Cat # 28341

  • TaqMan 2x RT-PCR Master Mix (3 Vials, 100 Reactions each) - Sufficient reagent for 100 x 20 µL reactions

 

Storage Conditions and Product Stability
Norgen’s TaqMan 2x PCR and 2x RT-PCR Master Mixes should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles. When stored at the proper temperature this reagent is stable for at least 1 year.