Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM39050 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the TCDVd Available in TaqMan format for analysis Tomato Chlorotic Dwarf Viroid (TCDVd) is a viroid which causes disease in both field and green-house tomatoes. The viroid is a single-stranded RNA molecule consisting of 300 nucleotides, and is closely related to the Potato Spindle Tuber Viroid. Infected tomato plants show different symptoms depending on the tomato variety, age of the plant, plant vigour and climatic conditions. Some symptoms of TCDVd infection include stunting, overall bunchiness, reduced leaf and fruit, leaf chlorosis, downward bending of leaves and even death of plants. TCDVd has been detected on tomato plants in Europe, Canada and the United States. The detection of TCDVd infection by symptoms is challenging since many other viroids and viruses can produce similar symptoms. Therefore, detection using PCR is the most effective method available. TaqMan PCR Kit Click to expand options TCDVd TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents TCDVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM39050 (100 preps) Cat. TM39010 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - TCDVd Primer & Probe Mix 280 μL 280 μL TCDVd Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM39050) TCDVd TaqMan RT-PCR Kit - Protocol (100 rxns) (TM39010) TCDVd RT-PCR Primer Set and Controls - Protocol (100 rxns) POSTERS Rapid Isolation and Sensitive Detection of the Chrysanthemum Chlorotic Mottle Viroid Related Products Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan Probe/Primer and Control Set Plant/Fungi Total RNA Purification Kit
Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM39050 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
