Purchasing Information


  • Detection kits for the TCDVd
  • Available in 2 formats for analysis:
 

TCDVd TaqMan RT-PCR Kit (Cat. TM39000) 24 rxns
TCDVd TaqMan Probe/Primer and Control Set (Cat. TM39010) 100 rxns
TCDVd End-Point RT-PCR Kit (Cat. EP39000) 24 rxns
TCDVd Primer and Control Set (Cat. EP39010) 100 rxns

  • Detection kits for the TCDVd
  • Available in 2 formats for analysis:
 


TCDVd Detection Kits

Tomato Chlorotic Dwarf Viroid (TCDVd) is a viroid which causes disease in both field and green-house tomatoes. The viroid is a single-stranded RNA molecule consisting of 300 nucleotides, and is closely related to the Potato Spindle Tuber Viroid. Infected tomato plants show different symptoms depending on the tomato variety, age of the plant, plant vigour and climatic conditions. Some symptoms of TCDVd infection include stunting, overall bunchiness, reduced leaf and fruit, leaf chlorosis, downward bending of leaves and even death of plants. TCDVd has been detected on tomato plants in Europe, Canada and the United States. The detection of TCDVd infection by symptoms is challenging since many other viroids and viruses can produce similar symptoms. Therefore, detection using PCR is the most effective method available.

There are 2 kits available for the detection of TCDVd:


Click to expand options
TCDVd TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
TCDVd TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
TCDVd End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
TCDVd End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


This product has a minimum of 10 per order.

TCDVd TaqMan RT-PCR Kit (Cat. TM39000) 24 rxns
TCDVd TaqMan Probe/Primer and Control Set (Cat. TM39010) 100 rxns
TCDVd End-Point RT-PCR Kit (Cat. EP39000) 24 rxns
TCDVd Primer and Control Set (Cat. EP39010) 100 rxns

  • Detection kits for the TCDVd
  • Available in 2 formats for analysis:
 


TCDVd Detection Kits

Tomato Chlorotic Dwarf Viroid (TCDVd) is a viroid which causes disease in both field and green-house tomatoes. The viroid is a single-stranded RNA molecule consisting of 300 nucleotides, and is closely related to the Potato Spindle Tuber Viroid. Infected tomato plants show different symptoms depending on the tomato variety, age of the plant, plant vigour and climatic conditions. Some symptoms of TCDVd infection include stunting, overall bunchiness, reduced leaf and fruit, leaf chlorosis, downward bending of leaves and even death of plants. TCDVd has been detected on tomato plants in Europe, Canada and the United States. The detection of TCDVd infection by symptoms is challenging since many other viroids and viruses can produce similar symptoms. Therefore, detection using PCR is the most effective method available.

There are 2 kits available for the detection of TCDVd:


Click to expand options
TCDVd TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
TCDVd TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
TCDVd End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
TCDVd End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

 

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