Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits

Cat. TM39050, TM39010
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Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits

Cat. TM39050, TM39010
  • Detection kits for the TCDVd
  • Available in TaqMan format for analysis
 

For research use only and NOT intended for in vitro diagnostics.

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TCDVd TaqMan RT-PCR Kit
(Cat. TM39050)
100 rxns
TCDVd TaqMan Probe/Primer and Control Set
(Cat. TM39010)
100 rxns

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Detection kits for the TCDVd
  • Available in TaqMan format for analysis
 

Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits

Tomato Chlorotic Dwarf Viroid (TCDVd) is a viroid which causes disease in both field and green-house tomatoes. The viroid is a single-stranded RNA molecule consisting of 300 nucleotides, and is closely related to the Potato Spindle Tuber Viroid. Infected tomato plants show different symptoms depending on the tomato variety, age of the plant, plant vigour and climatic conditions. Some symptoms of TCDVd infection include stunting, overall bunchiness, reduced leaf and fruit, leaf chlorosis, downward bending of leaves and even death of plants. TCDVd has been detected on tomato plants in Europe, Canada and the United States. The detection of TCDVd infection by symptoms is challenging since many other viroids and viruses can produce similar symptoms. Therefore, detection using PCR is the most effective method available.


Click to expand options
TCDVd TaqMan RT-PCR Kit, 100 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
TCDVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Kit Specifications

You have selected: Cat. TM39050

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

You have selected: Cat. TM39010

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

Store both the primer set mix and the positive control at -20°C upon arrival.

Supporting Data

Click for expanded view

Component Cat. TM39050 (100 preps) Cat. TM39010 (100 preps)
MDx TaqMan 2X PCR Master Mix 2 x 700 μL -
TCDVd Primer & Probe Mix 280 μL 280 μL
TCDVd Positive Control 150 μL 150 μL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1

Tomato Chlorotic Dwarf Viroid (TCDVd) TaqMan RT-PCR Detection Kits

Tomato Chlorotic Dwarf Viroid (TCDVd) is a viroid which causes disease in both field and green-house tomatoes. The viroid is a single-stranded RNA molecule consisting of 300 nucleotides, and is closely related to the Potato Spindle Tuber Viroid. Infected tomato plants show different symptoms depending on the tomato variety, age of the plant, plant vigour and climatic conditions. Some symptoms of TCDVd infection include stunting, overall bunchiness, reduced leaf and fruit, leaf chlorosis, downward bending of leaves and even death of plants. TCDVd has been detected on tomato plants in Europe, Canada and the United States. The detection of TCDVd infection by symptoms is challenging since many other viroids and viruses can produce similar symptoms. Therefore, detection using PCR is the most effective method available.


Click to expand options
TCDVd TaqMan RT-PCR Kit, 100 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
TCDVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. TM39050

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

You have selected: Cat. TM39010

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

Store both the primer set mix and the positive control at -20°C upon arrival.

Component Cat. TM39050 (100 preps) Cat. TM39010 (100 preps)
MDx TaqMan 2X PCR Master Mix 2 x 700 μL -
TCDVd Primer & Probe Mix 280 μL 280 μL
TCDVd Positive Control 150 μL 150 μL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1
 


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