Total Nucleic Acid Preservation Tubes (Cat. 69200)

Figure 2. Stability of RNA

Figure 2. Stability of RNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the RNase Protein (RP) gene from 5 oropharyngeal swab samples and 5 nasopharyngeal swab samples purified using Norgens Total RNA Purification Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan One-Step RT-PCR.

Figure 3. Stability of DNA

Figure 3. Stability of DNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the GAPDH gene from 5 oropharyngeal swab samples and from 5 nasopharyngeal swab samples purified using Norgen's Saliva DNA Isolation Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan PCR.

Figure 4. Inhibitory Effects

Figure 4. Inhibitory Effects of Norgens Total Nucleic Acid Preservative on the Growth of Bacteria, Yeast and Viruses. The effect of Norgens Total Nucleic Acid Preservative on the growth of microorganisms clearly demonstrated that Norgens preservative inhibits the growth of virus, bacteria and yeast, thereby resulting in non-infectious samples that can be handled and shipped safely.

Figure 5. Downstream Application of Preserved Swab RNA & DNA

Figure 5. Downstream Application of Preserved Swab RNA & DNA. The application of the RNA or DNA purified from preserved oropharyngeal swab samples was evaluated to detect RNase P (house keeping gene) and 16S rRNA (bacterial DNA) from swab samples collected from 60 donors and the preserved at room temperature overnight. Each individual swab sample contains different levels of cells, contributing to the variable Ct of each gene detection, but all passed the acceptance criteria for valid RT-PCR and PCR (< 35Ct, red line) in the 20 µL reaction, indicating that preserved swab RNA and DNA stored in Norgens Total Nucleic Acid Preservative is of high quality for downstream applications, such as real-time RT-PCR and PCR.