Tomato Planta Macho Viroid (TPMVd) TaqMan RT-PCR Detection Kits

Overview

Tomato Planta Macho Viroid (TPMVd) is the causal agent of a disease of tomato known as “Planta Macho” (male plant) disease, which occurs primarily in the Mexican states of Morelos and Mexico. Symptoms of the disease resemble those induced in tomato by the potato spindle tuber viroid, but are more severe. Infected plants are severely stunted and exhibit strong epinasty of leaves and leaflets. Infected plants produce more flowers and fruits than healthy plants, however the fruit remains small and has no commercial value. In the field the disease appears to be spread via contact with diseased foliage or contaminated tools. Norgen’s Tomato Planta Macho Viroid RT-PCR Detection Kit is a rapid and sensitive TPMVd detection tool which can be applicable for the early diagnosis of TPMVd.

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

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