• Detection kits for the TPMVd
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
TPMVd TaqMan RT-PCR Kit TM48000 24 rxns
TPMVd TaqMan Probe/Primer and Control Set TM48010 100 rxns
TPMVd End-Point RT-PCR Kit EP48000 24 rxns
TPMVd Primer and Control Set EP48010 100 rxns

TPMVd Detection Kits

Tomato Planta Macho Viroid (TPMVd) is the causal agent of a disease of tomato known as “Planta Macho” (male plant) disease, which occurs primarily in the Mexican states of Morelos and Mexico. Symptoms of the disease resemble those induced in tomato by the potato spindle tuber viroid, but are more severe. Infected plants are severely stunted and exhibit strong epinasty of leaves and leaflets. Infected plants produce more flowers and fruits than healthy plants, however the fruit remains small and has no commercial value. In the field the disease appears to be spread via contact with diseased foliage or contaminated tools. Norgen’s Tomato Planta Macho Viroid RT-PCR Detection Kit is a rapid and sensitive TPMVd detection tool which can be applicable for the early diagnosis of TPMVd.

There are 2 kits available for the detection of TPMVd:

TPMVd TaqMan RT-PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
TPMVd End-Point RT-PCR Kit
  • Master Mix and primers for the specific amplification of a 248 nucleotide region of the TPMVd genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM48000
(24 rxns)
End-Point
Cat. EP48000
(24 rxns)
MDx 2X RT-PCR Master Mix - 350 µL
MDx TaqMan 2X RT-PCR Master Mix 350 µL -
TPMVd Primer Mix 70 µL 70 µL
TPMVd Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM48010
(100 rxns)
Primer
and Control Set
Cat. EP48010
(100 rxns)
TPMVd Primer Mix 3 x 70 µL 3 x 70 µL
TPMVd Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1