Features and Benefits

TruScript Reverse Transcriptase is Available in Two Convenient Formats:

1. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
   This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
   The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.

2. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
   This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
   The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

Which TruScript Kit is Best for Your Needs?

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Supporting Data

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Figure 1. Robust Reverse Transcription of All RNA Transcripts. Norgen's TruScript Reverse Transcriptase is a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase that can produce cDNA from different kinds of RNA transcripts, including those with high difficulty or a high degree of secondary structure. In Panel A, TruScript Reverse Transcriptase was compared with a competitor's M-MuLV Reverse Transcriptase for RT-PCR of two different RNA transcripts. Norgen's TruScript showed good amplification for both targets while the competitor failed to amplify Target #2 with good quantity. In Panel B, TruScript Reverse Transcriptase was compared with a competitor's M-MuLV Reverse Transcriptase in an RT-qPCR reaction. Norgen's TruScript Reverse Transcriptase provided a better amplification as shown with a lower Ct value.

Figure 2. High Sensitivity with Robust Amplification from Different Amounts of RNA Input. Norgen’s Norgen’s TruScript Reverse Transcriptase was used in an RT-qPCR reaction to amplify the human 5S rRNA transcripts from a dilution series of HeLa total RNA isolated using Norgen’s Total RNA Purification Kit. The PCR target was robustly amplified from a broad range of RNA inputs, from the 10 ng down to 1 pg.

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