TruScript Reverse Transcriptase and Kits All-in-One, Ready-to-use First Strand cDNA Synthesis Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz TruScript Reverse Transcriptase and Kits All-in-One, Ready-to-use First Strand cDNA Synthesis Kits Price USD$375.00 SKU 54420 Format First Strand cDNA Synthesis Kit First Strand cDNA Synthesis Kit for mRNA Size 50 Reactions Quantity Overview Details Documentation Citations Overview Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reactionTime Savings - Set up RT reactions in a shorter time since less pipetting steps are requiredCost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kitsHigh Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR productsRobust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility TruScript Reverse Transcriptase is Available in Three Convenient Formats: 1. TruScript Reverse Transcriptase Kit (Cat.#54440) This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers. 2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420) This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts). The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts. 3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400) This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts). The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample. Which TruScript Kit is Best for Your Needs? TruScript Reverse Transcriptase TruScript First Strand cDNA Synthesis Kit for mRNA TruScript First Strand cDNA Synthesis Kit Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) Your template is enriched mRNA Your template is total RNA (poly A OR non-poly A-containing transcripts) Kit contains oligo dT Kit contains both oligo-dT primer and Random Hexamers Kit contains reaction buffer with nucleotides and primer with oligo-dT with oligo-dT and random hexamers You have your own first strand synthesis primer Your template is microRNA (using your own primers) Cat.#54440 Cat.#54400 Cat.#54420 Details Supporting Data Previous Figure 1. Robust Reverse Transcription of All RNA Transcripts. Norgen's TruScript Reverse Transcriptase is a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase that can produce cDNA from different kinds of RNA transcripts, including those with high difficulty or a high degree of secondary structure. In Panel A, TruScript Reverse Transcriptase was compared with a competitor's M-MuLV Reverse Transcriptase for RT-PCR of two different RNA transcripts. Norgen's TruScript showed good amplification for both targets while the competitor failed to amplify Target #2 with good quantity. In Panel B, TruScript Reverse Transcriptase was compared with a competitor's M-MuLV Reverse Transcriptase in an RT-qPCR reaction. Norgen's TruScript Reverse Transcriptase provided a better amplification as shown with a lower Ct value. Figure 2. High Sensitivity with Robust Amplification from Different Amounts of RNA Input. Norgen’s Norgen’s TruScript Reverse Transcriptase was used in an RT-qPCR reaction to amplify the human 5S rRNA transcripts from a dilution series of HeLa total RNA isolated using Norgen’s Total RNA Purification Kit. The PCR target was robustly amplified from a broad range of RNA inputs, from the 10 ng down to 1 pg. Next Click for expanded view TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) - Kit Components 1. TruScript Enzyme Mix 2. 2x Reaction Mix 3. Nuclease-Free Water 4. Product Insert Storage Conditions and Product Stability Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers. Component Cat. 54440 (10,000 units) Cat. 54420 (50 rxns) Cat. 54410 (50 rxns) TruScript™ Reverse Transcriptase (200 units/ μL) 50 μL - - 5x RT Buffer 1 mL - - TruScript™ Enzyme Mix - 100 μL 100 μL 2x Reaction Mix - 500 μL - 2x Reaction Mix for mRNA - - 500 μL Nuclease-Free Water 1.25 mL 1.25 mL 1.25 mL Product Insert 1 1 1 Documentation PROTOCOLS 54420 - TruScript First Strand cDNA Synthesis Kit Protocol (50 rxns) 54440 - TruScript Reverse Transcriptase Protocol (10,000 units) 54400 - TruScript First Strand cDNA Synthesis Kit for mRNA Protocol (50 rxns) APPLICATION NOTES Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device FLYERS Flyer Citations Title Expression of AQP8 in serum of patients with Meniere's disease and its value in evaluating the degree of hydrolabyrinth and predicting prognosis Journal Authorea. 2022. Authors Rui Zhao, Tianhua Yi, Qinqin Wu, Xuemei Liu, Jianqiao He, Yufang Tan Title In vivo assessment of salinity stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene Journal BIOLOGIA PLANTARUM. 2022. Authors Not Available Title Microfluidic-based mechanical phenotyping of cells for the validation of epithelial-to-mesenchymal-like transition caused by insufficient heat treatment Journal Sensors and Actuators B: Chemical. 2017. Authors Jiyoon Bu, Tae Hee Lee, In Sik Kim, Young-Ho Cho Title Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917 Journal FEMS Microbiology Letters. 2015. Authors Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW Related Products microScript microRNA cDNA Synthesis Kit
TruScript Reverse Transcriptase and Kits All-in-One, Ready-to-use First Strand cDNA Synthesis Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz TruScript Reverse Transcriptase and Kits All-in-One, Ready-to-use First Strand cDNA Synthesis Kits Price USD$375.00 SKU 54420 Format First Strand cDNA Synthesis Kit First Strand cDNA Synthesis Kit for mRNA Size 50 Reactions Quantity
