TruScript Reverse Transcriptase and Kits (Cat. 54440, 54420, 54400)
TruScript Reverse Transcriptase and Kits
![TruScript Reverse Transcriptase](https://norgenbiotek.com/sites/default/files/styles/original/public/images/product_images/54440-TruScript-Reverse-Transcriptase_WEB.jpg?itok=5Vcjt2AG" "TruScript Reverse Transcriptase")
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TruScript Reverse Transcriptase and Kits
All-in-One, Ready-to-use First Strand cDNA Synthesis Kits
- Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
- Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required
- Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
- High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
- Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 54440) 10,000 Units
(Cat. 54420) 50 Reactions
(Cat. 54400) 50 Reactions
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
TruScript Reverse Transcriptase (Cat.# 54440) - Kit Components
1. TruScript Reverse Transcriptase (200 units / μL)
2. 5x RT Buffer
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) - Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) - Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Supporting Data
| Title | Expression of AQP8 in serum of patients with Meniere's disease and its value in evaluating the degree of hydrolabyrinth and predicting prognosis |
| Journal | Authorea. 2022. |
| Authors | Rui Zhao, Tianhua Yi, Qinqin Wu, Xuemei Liu, Jianqiao He, Yufang Tan |
| Title | In vivo assessment of salinity stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene |
| Journal | BIOLOGIA PLANTARUM. 2022. |
| Authors | Not Available |
| Title | Microfluidic-based mechanical phenotyping of cells for the validation of epithelial-to-mesenchymal-like transition caused by insufficient heat treatment |
| Journal | Sensors and Actuators B: Chemical. 2017. |
| Authors | Jiyoon Bu, Tae Hee Lee, In Sik Kim, Young-Ho Cho |
| Title | Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917 |
| Journal | FEMS Microbiology Letters. 2015. |
| Authors | Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW |
TruScript Reverse Transcriptase and Kits
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
1. TruScript Reverse Transcriptase Kit (Cat.#54440)
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
Which TruScript Kit is Best for Your Needs?
| TruScript Reverse Transcriptase |
TruScript First Strand cDNA Synthesis Kit for mRNA |
TruScript First Strand cDNA Synthesis Kit |
|
| Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) |
|||
| Your template is enriched mRNA |
|||
| Your template is total RNA (poly A OR non-poly A-containing transcripts) |
|||
| Kit contains oligo dT | |||
| Kit contains both oligo-dT primer and Random Hexamers |
|||
| Kit contains reaction buffer with nucleotides and primer |
with oligo-dT |
with oligo-dT and random hexamers |
|
| You have your own first strand synthesis primer |
|||
| Your template is microRNA (using your own primers) |
|||
| Cat.#54440 | Cat.#54400 | Cat.#54420 |
Supporting Data
Kit Specifications
TruScript Reverse Transcriptase (Cat.# 54440) - Kit Components
1. TruScript Reverse Transcriptase (200 units / μL)
2. 5x RT Buffer
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) - Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) - Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
| Title | Expression of AQP8 in serum of patients with Meniere's disease and its value in evaluating the degree of hydrolabyrinth and predicting prognosis |
| Journal | Authorea. 2022. |
| Authors | Rui Zhao, Tianhua Yi, Qinqin Wu, Xuemei Liu, Jianqiao He, Yufang Tan |
| Title | In vivo assessment of salinity stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene |
| Journal | BIOLOGIA PLANTARUM. 2022. |
| Authors | Not Available |
| Title | Microfluidic-based mechanical phenotyping of cells for the validation of epithelial-to-mesenchymal-like transition caused by insufficient heat treatment |
| Journal | Sensors and Actuators B: Chemical. 2017. |
| Authors | Jiyoon Bu, Tae Hee Lee, In Sik Kim, Young-Ho Cho |
| Title | Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917 |
| Journal | FEMS Microbiology Letters. 2015. |
| Authors | Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW |