All-in-One, Ready-to-use First Strand cDNA Synthesis Kits

  • Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
  • Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required
  • Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
  • High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
  • Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility

ItemsCat. #Size
TruScript Reverse Transcriptase 54440 10,000 Units
TruScript First Strand cDNA Synthesis Kit 54420 50 Reactions
TruScript First Strand cDNA Synthesis Kit for mRNA 54400 50 Reactions

TruScript Reverse Transcriptase and Kits

TruScript Reverse Transcriptase is Available in Three Convenient Formats:

1. TruScript Reverse Transcriptase Kit (Cat.#54440)

This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.

2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.

3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

 

Which TruScript Kit is Best for Your Needs?

 

  TruScript
Reverse Transcriptase
TruScript
First Strand cDNA
Synthesis Kit
for mRNA
TruScript
First Strand cDNA
Synthesis Kit
Kit contains only reverse
transcriptase enzyme and
buffer (no primers or other
buffer components)
   
Your template is enriched
mRNA
   
Your template is total RNA
(poly A OR non-poly
A-containing transcripts)
 
Kit contains oligo dT    
Kit contains both oligo-dT
primer and Random
Hexamers
   
Kit contains reaction buffer
with nucleotides and primer
 
with oligo-dT
 

with oligo-dT and
random hexamers
You have your own first
strand synthesis primer
   
Your template is microRNA
(using your own primers)
   
  Cat.#54440 Cat.#54400 Cat.#54420

 

TruScript Reverse Transcriptase (Cat.# 54440) - Kit Components

1. TruScript Reverse Transcriptase (200 units / μL)
2. 5x RT Buffer
3. Nuclease-Free Water
4. Product Insert

TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) - Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert

TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) - Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.

Title Microfluidic-based mechanical phenotyping of cells for the validation of epithelial-to-mesenchymal-like transition caused by insufficient heat treatment
Journal Sensors and Actuators B: Chemical. 2017.
Authors Jiyoon Bu, Tae Hee Lee, In Sik Kim, Young-Ho Cho
Title Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917
Journal FEMS Microbiology Letters. 2015.
Authors Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW