TruScript Reverse Transcriptase and Kits
All-in-One, Ready-to-use First Strand cDNA Synthesis Kits
For research use only and NOT intended for in vitro diagnostics.
TruScript Reverse Transcriptase and Kits
All-in-One, Ready-to-use First Strand cDNA Synthesis Kits
Register today to receive an exclusive 15% off* on your first order.
Overview
- Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
- Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required
- Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
- High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
- Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
1. TruScript Reverse Transcriptase Kit (Cat.#54440)
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
Which TruScript Kit is Best for Your Needs?
TruScript Reverse Transcriptase |
TruScript First Strand cDNA Synthesis Kit for mRNA |
TruScript First Strand cDNA Synthesis Kit |
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Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) |
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Your template is enriched mRNA |
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Your template is total RNA (poly A OR non-poly A-containing transcripts) |
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Kit contains oligo dT | |||
Kit contains both oligo-dT primer and Random Hexamers |
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Kit contains reaction buffer with nucleotides and primer |
with oligo-dT |
with oligo-dT and random hexamers |
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You have your own first strand synthesis primer |
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Your template is microRNA (using your own primers) |
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Cat.#54440 | Cat.#54400 | Cat.#54420 |
Details
Supporting Data
TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) - Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 54440 (10,000 units) | Cat. 54420 (50 rxns) | Cat. 54410 (50 rxns) |
---|---|---|---|
TruScript™ Reverse Transcriptase (200 units/ μL) | 50 μL | - | - |
5x RT Buffer | 1 mL | - | - |
TruScript™ Enzyme Mix | - | 100 μL | 100 μL |
2x Reaction Mix | - | 500 μL | - |
2x Reaction Mix for mRNA | - | - | 500 μL |
Nuclease-Free Water | 1.25 mL | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 | 1 |
Documentation
Citations
Title | IL-1ß induced down-regulation of miR-146a-5p promoted pyroptosis and apoptosis of corneal epithelial cell in dry eye disease through targeting STAT3 |
Citation | BMC Ophthalmology 2023. |
Authors | Xuejiao Li, Hua Peng, Jianshu Kang, Xiaomei Sun & Jian Liu |
Title | Molecular cloning, characterization and expression profile of FLOWERING LOCUS T (FT) gene from Prunus armeniaca L. |
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Citation | bioRxiv 2023. |
Authors | Mohammad, Mohammad Ghaleb, El-Serafi, Ahmed, Madkour, Mohamed Ibrahim, Alhabshi, Abeer, Wadea, Ansar, Jabal, Rola Abu, Sandeep, Divyasree, Soliman, Sameh S. M. |
Title | The Effects of Pifithrin-µ on Spermatogonial Stem Cell Viability and Pluripotency |
Citation | Highlights in human heredity 2023. |
Authors | Sara Moghadasi, Ehsan Razeghian, Mehdi Shamsara and Farid Heidari |
Title | Validación de una nueva estrategia para la identificación de variantes de SARS-CoV-2 mediante secuenciación del gen espiga por Sanger |
Citation | Enfermedades Infecciosas y Microbiología Clínica 2023. |
Authors | Enderson Murillo, Katherine Palacio-Rua, Carlos Afanador-Ayala, Juan Felipe García-Correa, Andrés F. Zuluaga |
Title | Validation of a new strategy for the identification of SARS-CoV-2 variants by sequencing the spike gene by SangerValidación de una nueva estrategia para la identificación de variantes de SARS-CoV-2 mediante secuenciación del gen Espiga por Sanger |
Citation | Enfermedades infecciosas y microbiologia clinica (English ed.) 2023. |
Authors | Enderson Murillo, Katherine Palacio-Rua, Carlos Afanador-Ayala, Juan Felipe García-Correa, Andrés F. Zuluaga |