TruScript Reverse Transcriptase and Kits

Cat. 54440, 54420, 54400
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TruScript Reverse Transcriptase and Kits

Cat. 54440, 54420, 54400

All-in-One, Ready-to-use First Strand cDNA Synthesis Kits

  • Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
  • Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required
  • Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
  • High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
  • Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility

For research use only and NOT intended for in vitro diagnostics.

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TruScript Reverse Transcriptase
(Cat. 54440)
10,000 Units
TruScript First Strand cDNA Synthesis Kit
(Cat. 54420)
50 Reactions
TruScript First Strand cDNA Synthesis Kit for mRNA
(Cat. 54400)
50 Reactions

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
  • Time Savings - Set up RT reactions in a shorter time since less pipetting steps are required
  • Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
  • High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
  • Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility

TruScript Reverse Transcriptase and Kits

TruScript Reverse Transcriptase is Available in Three Convenient Formats:

1. TruScript Reverse Transcriptase Kit (Cat.#54440)

This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.

2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.

3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

 

Which TruScript Kit is Best for Your Needs?

 

  TruScript
Reverse Transcriptase
TruScript
First Strand cDNA
Synthesis Kit
for mRNA
TruScript
First Strand cDNA
Synthesis Kit
Kit contains only reverse
transcriptase enzyme and
buffer (no primers or other
buffer components)
   
Your template is enriched
mRNA
   
Your template is total RNA
(poly A OR non-poly
A-containing transcripts)
 
Kit contains oligo dT    
Kit contains both oligo-dT
primer and Random
Hexamers
   
Kit contains reaction buffer
with nucleotides and primer
 
with oligo-dT
 

with oligo-dT and
random hexamers
You have your own first
strand synthesis primer
   
Your template is microRNA
(using your own primers)
   
  Cat.#54440 Cat.#54400 Cat.#54420

 

Kit Specifications

You have selected: Cat. 54440

TruScript Reverse Transcriptase (Cat.# 54440) Kit Components

1. TruScript Reverse Transcriptase (200 units / μL)
2. 5x RT Buffer
3. Nuclease-Free Water
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. 54420

TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water 
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. 54400

TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) - Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water 
4. Product Insert

 

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

Supporting Data

Click for expanded view

Component Cat. 54440 (10,000 units) Cat. 54420 (50 rxns) Cat. 54410 (50 rxns)
TruScript™ Reverse Transcriptase (200 units/ μL) 50 μL - -
5x RT Buffer 1 mL - -
TruScript™ Enzyme Mix - 100 μL 100 μL
2x Reaction Mix - 500 μL -
2x Reaction Mix for mRNA - - 500 μL
Nuclease-Free Water 1.25 mL 1.25 mL 1.25 mL
Product Insert 1 1 1

Title Expression of AQP8 in serum of patients with Meniere's disease and its value in evaluating the degree of hydrolabyrinth and predicting prognosis
Journal Authorea. 2022.
Authors Rui Zhao, Tianhua Yi, Qinqin Wu, Xuemei Liu, Jianqiao He, Yufang Tan
Title Microfluidic-based mechanical phenotyping of cells for the validation of epithelial-to-mesenchymal-like transition caused by insufficient heat treatment
Journal Sensors and Actuators B: Chemical. 2017.
Authors Jiyoon Bu, Tae Hee Lee, In Sik Kim, Young-Ho Cho
Title Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917
Journal FEMS Microbiology Letters. 2015.
Authors Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW

TruScript Reverse Transcriptase and Kits

TruScript Reverse Transcriptase is Available in Three Convenient Formats:

1. TruScript Reverse Transcriptase Kit (Cat.#54440)

This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.

2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.

3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

 

Which TruScript Kit is Best for Your Needs?

 

  TruScript
Reverse Transcriptase
TruScript
First Strand cDNA
Synthesis Kit
for mRNA
TruScript
First Strand cDNA
Synthesis Kit
Kit contains only reverse
transcriptase enzyme and
buffer (no primers or other
buffer components)
   
Your template is enriched
mRNA
   
Your template is total RNA
(poly A OR non-poly
A-containing transcripts)
 
Kit contains oligo dT    
Kit contains both oligo-dT
primer and Random
Hexamers
   
Kit contains reaction buffer
with nucleotides and primer
 
with oligo-dT
 

with oligo-dT and
random hexamers
You have your own first
strand synthesis primer
   
Your template is microRNA
(using your own primers)
   
  Cat.#54440 Cat.#54400 Cat.#54420

 

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 54440

TruScript Reverse Transcriptase (Cat.# 54440) Kit Components

1. TruScript Reverse Transcriptase (200 units / μL)
2. 5x RT Buffer
3. Nuclease-Free Water
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. 54420

TruScript First Strand cDNA Synthesis Kit (Cat.# 54420) Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water 
4. Product Insert

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

You have selected: Cat. 54400

TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) - Kit Components

1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water 
4. Product Insert

 

Storage Conditions and Product Stability

Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C.  These reagents should remain stable for at least 1 year in their unopened containers.

Component Cat. 54440 (10,000 units) Cat. 54420 (50 rxns) Cat. 54410 (50 rxns)
TruScript™ Reverse Transcriptase (200 units/ μL) 50 μL - -
5x RT Buffer 1 mL - -
TruScript™ Enzyme Mix - 100 μL 100 μL
2x Reaction Mix - 500 μL -
2x Reaction Mix for mRNA - - 500 μL
Nuclease-Free Water 1.25 mL 1.25 mL 1.25 mL
Product Insert 1 1 1

Title Expression of AQP8 in serum of patients with Meniere's disease and its value in evaluating the degree of hydrolabyrinth and predicting prognosis
Journal Authorea. 2022.
Authors Rui Zhao, Tianhua Yi, Qinqin Wu, Xuemei Liu, Jianqiao He, Yufang Tan
Title Microfluidic-based mechanical phenotyping of cells for the validation of epithelial-to-mesenchymal-like transition caused by insufficient heat treatment
Journal Sensors and Actuators B: Chemical. 2017.
Authors Jiyoon Bu, Tae Hee Lee, In Sik Kim, Young-Ho Cho
Title Recombinant production of omega-3 fatty acids by probiotic Escherichia coli Nissle 1917
Journal FEMS Microbiology Letters. 2015.
Authors Amiri-Jami M, Abdelhamid AG, Hazaa M, Kakuda Y, Griffths MW

 


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