Urine Cell-Free Circulating DNA Purification Kits

Cat. 56600, 56700, 56800
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Urine Cell-Free Circulating DNA Purification Kits

Cat. 56600, 56700, 56800

Purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA

  • Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples
  • Isolate viral DNA
  • Versatile urine input volumes
  • Concentrate circulating DNA into flexible elution volumes
  • Isolate inhibitor-free cell-free circulating DNA
  • Compatible with Norgen’s Urine Preservative and other commercially available urine preservatives
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

#TogetherWithNorgen

  • For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
  • Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
  • Learn More by clicking here.

For research use only and NOT intended for in vitro diagnostics.

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Urine Cell-Free Circulating DNA Purification Mini Kit
(Cat. 56600)
50 preps
Urine Cell-Free Circulating DNA Purification Midi Kit
(Cat. 56700)
20 preps
Urine Cell-Free Circulating DNA Purification Maxi Kit
(Cat. 56800)
10 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples
  • Isolate viral DNA
  • Versatile urine input volumes
  • Concentrate circulating DNA into flexible elution volumes
  • Isolate inhibitor-free cell-free circulating DNA
  • Compatible with Norgen’s Urine Preservative and other commercially available urine preservatives
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

#TogetherWithNorgen

  • For every purchase of Urine kits during November, 10% of the proceeds will be donated to charity by Norgen.
  • Take a selfie wearing a Norgen moustache and share it using the hashtag #TogetherWithNorgen, and we will donate $10 in your name to charity.
  • Learn More by clicking here.

Urine Cell-Free Circulating DNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.

Background

Recent evidence indicates that cell-free circulating DNA (cf-DNA) contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood). Urine cell-free circulating DNA (cf-DNA) has been utilized for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases, as well as for investigating fetal DNA that is normally present in the maternal blood. This cf-DNA is usually present as short fragments, generally between 150 and 250 bp or its duplicates, and is derived either from the apoptotic process, necrotic process or from the fetus.

Urine Cell-Free Circulating DNA Purification Mini Kit

For sample volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating DNA Purification Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Workflow

Urine Cell-Free Circulating DNA Purification Maxi Kit

For sample volumes ranging from 10 mL to 30 mL.

Kit Specifications

You have selected: Cat. 56600
Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
2 mL
Size of DNA Purified
All sizes of
DNA ≥ 50 bp
Elution Volume
50-100 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 56700
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of DNA Purified
All sizes of
DNA ≥ 50 bp
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 56800
Kit Specifications
Minimum Urine Input
10 mL
Maximum Urine Input
30 mL
Size of DNA Purified
All sizes of
DNA ≥ 50 bp
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Supporting Data

Click for expanded view

Component Cat. 56600 (50 preps) Cat. 56700 (20 preps) Cat. 56800 (10 preps)
Binding Solution K 25 mL 75 mL 1 x 75 mL
1 x 25 mL
Proteinase K 1.2 mL 0.3 mL 0.3 mL
Wash Solution A 38 mL 2 x 38 mL 38 mL
Elution Buffer B 8 mL 15 mL 15 mL
Mini Spin Columns 50 20 10
Midi Spin Columns - 20 -
Maxi Spin Columns - - 10
Collection Tubes 50 20 10
Elution Tubes (1.7 mL) 50 20 10
Product Insert 1 1 1

Mini (56600)

Midi (56700)

Title Measurement Biases Distort Cell-Free DNA Fragmentation Profiles and Define the Sensitivity of Metagenomic Cell-Free DNA Sequencing Assays
Journal Clinical Chemistry. 2021.
Authors Chang A, Mzava O, Lenz JS, et al.
Title Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions
Journal Diagnostics (Basel). 2020.
Authors Eun Young Lee, Eun-Ju Lee, Hana Yoon, Dong Hyeon Lee, and Kwang Hyun Kim

Maxi (56800)

Urine Cell-Free Circulating DNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.

Background

Recent evidence indicates that cell-free circulating DNA (cf-DNA) contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood). Urine cell-free circulating DNA (cf-DNA) has been utilized for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases, as well as for investigating fetal DNA that is normally present in the maternal blood. This cf-DNA is usually present as short fragments, generally between 150 and 250 bp or its duplicates, and is derived either from the apoptotic process, necrotic process or from the fetus.

Urine Cell-Free Circulating DNA Purification Mini Kit

For sample volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating DNA Purification Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Workflow

Urine Cell-Free Circulating DNA Purification Maxi Kit

For sample volumes ranging from 10 mL to 30 mL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 56600
Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
2 mL
Size of DNA Purified
All sizes of
DNA ≥ 50 bp
Elution Volume
50-100 μL
Time to Complete 10 Purifications
15-20 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 56700
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of DNA Purified
All sizes of
DNA ≥ 50 bp
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

You have selected: Cat. 56800
Kit Specifications
Minimum Urine Input
10 mL
Maximum Urine Input
30 mL
Size of DNA Purified
All sizes of
DNA ≥ 50 bp
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component Cat. 56600 (50 preps) Cat. 56700 (20 preps) Cat. 56800 (10 preps)
Binding Solution K 25 mL 75 mL 1 x 75 mL
1 x 25 mL
Proteinase K 1.2 mL 0.3 mL 0.3 mL
Wash Solution A 38 mL 2 x 38 mL 38 mL
Elution Buffer B 8 mL 15 mL 15 mL
Mini Spin Columns 50 20 10
Midi Spin Columns - 20 -
Maxi Spin Columns - - 10
Collection Tubes 50 20 10
Elution Tubes (1.7 mL) 50 20 10
Product Insert 1 1 1

Mini (56600)

Midi (56700)

Title Measurement Biases Distort Cell-Free DNA Fragmentation Profiles and Define the Sensitivity of Metagenomic Cell-Free DNA Sequencing Assays
Journal Clinical Chemistry. 2021.
Authors Chang A, Mzava O, Lenz JS, et al.
Title Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions
Journal Diagnostics (Basel). 2020.
Authors Eun Young Lee, Eun-Ju Lee, Hana Yoon, Dong Hyeon Lee, and Kwang Hyun Kim

Maxi (56800)

 


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