For the rapid concentration of total proteins from up to 20mL urine samples

  • Simultaneous concentration and desalting of total urinary proteins
  • No molecular weight cutoff, therefore isolate all sizes of proteins and peptides
  • Process 4 samples in only 40 minutes
  • Each column can concentrate and desalt up to 4 mg of urinary protein

ItemsCat. #Size
ProteoSpin™ Urine Protein Concentration Maxi Kit 21600 4 preps

ProteoSpin™ Urine Protein Concentration Maxi Kit

This kit concentrates urine proteins while simultaneously removing salts, urea, and other urine contaminants. Since there is no molecular weight cut-off these columns will capture total urinary proteins and peptides of all sizes, making them ideal for biomarker discovery work or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. Each spin column is able to concentrate and desalt up to 4 mg of urine proteins.

The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the protein conveniently for downstream applications including SDS-PAGE, 2D SDS-PAGE, Whole protein mass spectrometry, MALDI-TOF, LC/MS, LC/MS/MS, Western blotting and Protein microarrays. Urinary proteins are important for many applications such as biomarker discovery, differential expression of proteins in various diseases and diagnosis.

Kit Specifications
Maximum Urine Input Volume
20 mL
Maximum Recovered Protein
4 mg
Minimum Elution Volume
2 mL
Time to Process 4 Samples
45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C.


Kit Components
Component Cat. 21600 (4 preps)
Wash Solution C 130 mL
Binding Buffer A 8 mL
Elution Buffer C 30 mL
Protein Neutralizer 4 mL
Maxi Spin Columns (assembled with collection tubes) 4
Elution Tubes (50 mL) 4
Product Insert 1

Title Treatment of Collagen Defects Using Protein Solutions
Journal United States Patent Application 20160166645. 2016.
Authors Matuska A, O'shaughnessey K, Woodell-may JE
Title Methods of Treating Pain Using Protein Solutions
Journal United States Patent Application 20160136245. 2016.
Authors Toler K, King W, Woodell-may JE
Title Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins.
Journal Journal of Proteomics. 2015.
Authors Bathla S, Rawat P, Baithalu R, Yadav ML, Naru J, Tiwari A, Kumar S, Balhara AK, Singh S, Chaudhary S, Kumar R, Lotfan M, Behare P, Phulia SK, Mohanty TK, Kaushik JK, Nallapeta S, Singh I, Ambatipudi SK, Mohanty AK
Title Plasmin activates epithelial Na+ channels by cleaving the gamma subunit.
Journal Journal of Biological Chemistry. 2008.
Authors Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.
Title Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS.
Journal Proteomics Clinical Applications. 2007.
Authors Chen YT, Tsao CY, Li JM, Tsai CY, Chiu SF, Tseng TL.