For the rapid concentration of total proteins from up to 20mL urine samples

  • Simultaneous concentration and desalting of total urinary proteins
  • No molecular weight cutoff, therefore isolate all sizes of proteins and peptides
  • Process 4 samples in only 40 minutes
  • Each column can concentrate and desalt up to 4 mg of urinary protein

ItemsCat. #Size
ProteoSpin™ Urine Protein Concentration Maxi Kit 21600 4 preps

ProteoSpin™ Urine Protein Concentration Maxi Kit

This kit concentrates urine proteins while simultaneously removing salts, urea, and other urine contaminants.  Since there is no molecular weight cut-off these columns will capture total urinary proteins and peptides of all sizes, making them ideal for biomarker discovery work or other diagnostic research.  The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. Each spin column is able to concentrate and desalt up to 4 mg of urine proteins. 

The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the protein conveniently for downstream applications including SDS-PAGE, 2D SDS-PAGE, Whole protein mass spectrometry, MALDI-TOF, LC/MS, LC/MS/MS, Western blotting and Protein microarrays.  Urinary proteins are important for many applications such as biomarker discovery, differential expression of proteins in various diseases and diagnosis. 

Kit Specifications
Maximum Urine Input Volume
20 mL
Maximum Recovered Protein
4 mg
Minimum Elution Volume
2 mL
Time to Process 4 Samples
45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C.

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