For the rapid concentration of total proteins from up to 20mL urine samples
|ProteoSpin™ Urine Protein Concentration Maxi Kit||21600||4 preps|
ProteoSpin™ Urine Protein Concentration Maxi Kit
This kit concentrates urine proteins while simultaneously removing salts, urea, and other urine contaminants. Since there is no molecular weight cut-off these columns will capture total urinary proteins and peptides of all sizes, making them ideal for biomarker discovery work or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. Each spin column is able to concentrate and desalt up to 4 mg of urine proteins.
The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the protein conveniently for downstream applications including SDS-PAGE, 2D SDS-PAGE, Whole protein mass spectrometry, MALDI-TOF, LC/MS, LC/MS/MS, Western blotting and Protein microarrays. Urinary proteins are important for many applications such as biomarker discovery, differential expression of proteins in various diseases and diagnosis.
|Maximum Urine Input Volume||
|Maximum Recovered Protein||
|Minimum Elution Volume||
|Time to Process 4 Samples||
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C.
|Title||Treatment of Collagen Defects Using Protein Solutions|
|Journal||United States Patent Application 20160166645. 2016.|
|Authors||Matuska A, O'shaughnessey K, Woodell-may JE|
|Title||Methods of Treating Pain Using Protein Solutions|
|Journal||United States Patent Application 20160136245. 2016.|
|Authors||Toler K, King W, Woodell-may JE|
|Title||Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins.|
|Journal||Journal of Proteomics. 2015.|
|Authors||Bathla S, Rawat P, Baithalu R, Yadav ML, Naru J, Tiwari A, Kumar S, Balhara AK, Singh S, Chaudhary S, Kumar R, Lotfan M, Behare P, Phulia SK, Mohanty TK, Kaushik JK, Nallapeta S, Singh I, Ambatipudi SK, Mohanty AK|
|Title||Plasmin activates epithelial Na+ channels by cleaving the gamma subunit.|
|Journal||Journal of Biological Chemistry. 2008.|
|Authors||Lu CY, Chang YM, Tseng WL, Feng CH, Lu CY.|
|Title||Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS.|
|Journal||Proteomics Clinical Applications. 2007.|
|Authors||Chen YT, Tsao CY, Li JM, Tsai CY, Chiu SF, Tseng TL.|
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