Urine DNA Isolation Kits
Fast and reliable purification of genomic and apoptotic DNA from urine
For research use only and NOT intended for in vitro diagnostics.
CE-IVDD marked diagnostic slurry format available here
Urine DNA Isolation Kits
Fast and reliable purification of genomic and apoptotic DNA from urine
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Features and Benefits
- Rapid isolation of both small and large species of DNA from urine
- Convenient spin column format
- Effective removal of PCR inhibitors
- Purified DNA is highly suited to sensitive downstream applications
- Allows for the purification of viral DNA from urine
Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.
Urine DNA Isolation Kit
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Preparation time for a single sample is about 30 minutes.
Urine DNA Isolation Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Preparation time for a single sample is less than 30 minutes.
Urine DNA Isolation Maxi Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Preparation time for a single sample is less than 30 minutes.
Background
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
Details
Supporting Data
Kit Specifications - Spin Column
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Minimum Urine Input | 3 mL |
Maximum Urine Input | 25 mL |
Time to Complete 10 Purifications | < 30 minutes |
Size of Urine DNA Purified | Large (> 1 kb) and small (150-250 bp) |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Slurry B1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry B1 contains a grey resin that will not dissolve when warmed.
Component | Cat. 18100 (50 preps) | Cat. 48800 (50 preps) | Cat. 50100 (50 preps) |
---|---|---|---|
Binding Solution K | 15 mL | - | - |
Slurry B1 | - | 18 mL | 18 mL |
Proteinase K in Storage Buffer | 2 mL | - | - |
Pronase K in Storage Buffer | 2 mL | - | - |
Soluton WN | 9 mL | - | - |
Binding Buffer A | - | - | 50 mL |
Lysis Buffer A | - | 30 mL | 30 mL |
Wash Solution A | - | 38 mL | 38 mL |
Wash Solution B | 30 mL | - | - |
Wash Solution D | 9 mL | - | - |
Binding Solution K | 15 mL | - | - |
Elution Buffer B | 15 mL | 15 mL | 15 mL |
Micro Spin Columns | 50 | - | - |
Mini Filter Spin Columns | - | 50 | 50 |
Collection Tubes | 50 | 50 | 50 |
Elution Tubes (1.7 mL) | 100 | 100 | 100 |
Product Insert | 1 | 1 | 1 |
Documentation
A Novel Method To Capture Methylated Human DNA From Urine Implications For Prostate Cancer Screening - Ascb 2008
The Urinary Genomic and Proteomic Profiling of Hepatocellular Carcinoma Patients Infected With Hepatitis C Virus - ASM 2008
Sensitivity of DNA Extraction Methods from Different Bodily Fluids for Human Identification
FAQs
Spin Column, Slurry, Maxi Slurry
RPM= √RCF/(1.118x10-5)(r)
Where RCF = required gravitational acceleration (relative centrifugal force in units of g); r = radius of the rotor in cm; and RPM = the number of revolutions per minute required to achieve the necessary g-force.
We recommend the following steps to prepare frozen urine for isolation:
- Gently warm the sample to room temperature or 37°C for 5 min.
- DO NOT perform a centrifugation step - this will eliminate the precipitated proteins leading to loss of protein-bound cf-NA or exosomes.
- Proceed with the protocol.
We recommend the use of Norgen’s Urine Preservative when collecting urine samples. Norgen’s Urine Preservative is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures, therefore no protein precipitation will occur and the purified nucleic acids will be of a higher quality.
Citations
Title | ColiSeq: a multiplex amplicon assay that provides strain level resolution of Escherichia coli directly from clinical specimens |
Citation | Microbiology Spectrum 2024. |
Authors | Charles H. D. Williamson,1 Adam J. Vazquez,1 Amalee E. Nunnally,1 Kristen Kyger,1 Viacheslav Y. Fofanov,1,2 Tara N. Furstenau,1,2 Heidie M. Hornstra,1 Joel Terriquez,3 Paul Keim,1 Jason W. Sahl |
Title | Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy |
Citation | Equine Veterinary Journal 2023. |
Authors | Ana Velloso Alvarez, E. Jose-Cunilleras, Abel Dorrego-Rodriguez, Isabel Santiago-Llorente, Maria de la Cuesta-Torrado, Lucas Troya-Portillo, Belen Rivera, Valentina Vitale, Lucia de Juan, Fatima Cruz-Lopez |
Title | DNA Capture and Enrichment: A Culture-Independent Approach for Characterizing the Genomic Diversity of Pathogenic Leptospira Species |
Citation | Microorganisms 2023. |
Authors | Nathan E. Stone, Ryelan F. McDonough, Camila Hamond, Karen LeCount, Joseph D. Busch, Katherine L. Dirsmith, Sarai Rivera-Garcia, Fred Soltero, Laura M. Arnold, Zachary Weiner, Renee L. Galloway, Lina K. Schlater, Jarlath E. Nally, Jason W. Sahl and David M. Wagne |
Title | When Plaquing Is Not Possible: Computational Methods for Detecting Induced Phages |
Citation | Viruses 2023. |
Authors | Taylor Miller-Esminger, Genevieve Johnson, Swarnali Banerjee and Catherine Putonti |
Title | Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo) |
Citation | mBio 2016. |
Authors | Alexander, K. A., Sanderson, C. E., Larsen, M. H., Robbe-Austerman, S., Williams, M. C., & Palmer, M. V |
Title | Multigene Methylation Analysis And The Noninvasive Diagnosis Of Prostate Cancer From Body Fluids |
Citation | European Scientific Journal 2016. |
Authors | Raluca, D., Anca, T., Ionescu, D., Florin, R. A., & Razvan, B |
Title | The potential use of urine cell free DNA as a marker for cancer |
Citation | Expert Review of Molecular Diagnostics 2016. |
Authors | Salvi, S., Martignano, F., Molinari, C., Gurioli, G., Calistri, D., De Giorgi, U., ... & Casadio, V |
Title | Urinary Mitochondrial DNA Copy Number Identifies Chronic Renal Injury in Hypertensive Patients |
Citation | Hypertension 2016. |
Authors | Eirin, A., Saad, A., Tang, H., Herrmann, S. M., Woollard, J. R., Lerman, A., ... & Lerman, L. O |
Title | Epigenetic silencing of the MUPCDH gene as a possible prognostic biomarker for cyst growth in ADPKD |
Citation | Scientific Reports 2015. |
Authors | Yu Mi Woo1 , Yubin Shin1 , Jung-Ah Hwang2 , Young-Hwan Hwang3 , Sunyoung Lee1 , EunYoung Park1 , Hyun Kyung Kong1 , Hayne Cho Park4, Yeon-Su Lee2 & Jong Hoon Park1 |
Title | Multiplex PCR assay for the simultaneous detection of C. perfringens, P. aeruginosa and K. pneumoniae |
Citation | Pathogenesis 2015. |
Authors | Pradeepkiran Jangampalli Adi, Ramesh Babu Pappithi, Praveen Chakravarthi Veeraraghavulu, Bhaskar Matcha |