For the rapid and efficient concentration of total proteins from up to 5 mL of urine

  • Simultaneous concentration, desalting and buffer exchange of total urinary proteins
  • No molecular weight cutoff, therefore isolate all sizes of proteins and peptides
  • Process up to 10 samples in only 30 minutes

ItemsCat. #Size
ProteoSpin™ Urine Protein Concentration Midi Kit 52300 10 preps

ProteoSpin™ Urine Protein Concentration Midi Kit

This kit concentrates urine proteins while simultaneously removing salts, urea, and other contaminants from urine. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work or other diagnostic research. Spin columns offer convenience, speed, and ease-of-use and thus significant time savings over classic dialysis protocols. Each spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream applications including SDS-PAGE, 2D gels, Whole protein mass spectrometry, MALDI-TOF, LC/MS, LC/MS/MS, Western blotting and Protein microarray applications and more. Urinary proteins are important for many application such as biomarker discovery, differential expression of proteins in various diseases, and diagnosis.

Kit Specifications
Minimum Urine Input
1 mL
Maximum Urine Input
5 mL
Maximum Recovered Proteins (5 mL Urine)
3 mg*
Elution Volume
200 - 1000 μL
Time to Complete 10 Purifications
30 minutes

*Yield will depend on the health status of the donor.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. Once opened, solutions should be stored at 4°C.


Kit Components
Component Cat. 52300 (10 preps)
Wash Solution C 60 mL
Binding Buffer A 4 mL
Elution Buffer C 30 mL
Protein Neutralizer 4 mL
Midi Spin Columns (assembled with collection tubes) 10
Midi Elution Tubes 10
Product Insert 1