For the rapid and efficient concentration of total proteins from up to 5 mL of urine
Items | Cat. # | Size | |
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ProteoSpin™ Urine Protein Concentration Midi Kit | 52300 | 10 preps |
ProteoSpin™ Urine Protein Concentration Midi Kit
This kit concentrates urine proteins while simultaneously removing salts, urea, and other contaminants from urine. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work or other diagnostic research. Spin columns offer convenience, speed, and ease-of-use and thus significant time savings over classic dialysis protocols. Each spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.
The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream applications including SDS-PAGE, 2D gels, Whole protein mass spectrometry, MALDI-TOF, LC/MS, LC/MS/MS, Western blotting and Protein microarray applications and more. Urinary proteins are important for many application such as biomarker discovery, differential expression of proteins in various diseases, and diagnosis.
Kit Specifications
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Minimum Urine Input
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1 mL
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Maximum Urine Input
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5 mL
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Maximum Recovered Proteins (5 mL Urine)
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3 mg*
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Elution Volume |
200 - 1000 μL
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Time to Complete 10 Purifications
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30 minutes
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*Yield will depend on the health status of the donor.
Kit Components | ||||||||||||||||
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