ProteoSpin™ Urine Protein Concentration Midi Kit (Cat. 52300)

For the rapid and efficient concentration of total proteins from up to 5 mL of urine

Simultaneous concentration, desalting and buffer exchange of total urinary proteins
No molecular weight cutoff, therefore isolate all sizes of proteins and peptides
Process up to 10 samples in only 30 minutes

Items	Cat. #	Size
ProteoSpin™ Urine Protein Concentration Midi Kit	52300	10 preps

ProteoSpin™ Urine Protein Concentration Midi Kit

This kit concentrates urine proteins while simultaneously removing salts, urea, and other contaminants from urine. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work or other diagnostic research. Spin columns offer convenience, speed, and ease-of-use and thus significant time savings over classic dialysis protocols. Each spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream applications including SDS-PAGE, 2D gels, Whole protein mass spectrometry, MALDI-TOF, LC/MS, LC/MS/MS, Western blotting and Protein microarray applications and more. Urinary proteins are important for many application such as biomarker discovery, differential expression of proteins in various diseases, and diagnosis.

Kit Specifications
Minimum Urine Input	1 mL
Maximum Urine Input	5 mL
Maximum Recovered Proteins (5 mL Urine)	3 mg*
Elution Volume	200 - 1000 μL
Time to Complete 10 Purifications	30 minutes

*Yield will depend on the health status of the donor.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. Once opened, solutions should be stored at 4°C.

Kit Components

Component	Cat. 52300 (10 preps)
Wash Solution C	60 mL
Binding Buffer A	4 mL
Elution Buffer C	30 mL
Protein Neutralizer	4 mL
Midi Spin Columns (assembled with collection tubes)	10
Midi Elution Tubes	10
Product Insert	1