Xylella fastidiosa TaqMan PCR Detection Kits
For research use only and NOT intended for in vitro diagnostics.
Lyophilized
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Xylella fastidiosa TaqMan PCR Detection Kits
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Overview
- Detection kits for the Xylella fastidiosa
- Available in TaqMan format for analysis
Xylella fastidiosa is an Gram-negative bacteria and is transmitted by xylem fluid feeding sap insects. Diseases related with X. fastidiosa are bacterial leaf scorch, oleander leaf scorch, coffee leaf scorch (CLS), alfalfa dwarf, phony peach disease, and the economically important Pierce’s disease of grapes (PD) , citrus variegated chlorosis (CVC) and olive quick decline syndrome (OQDS) in Europe. X. fastidiosa is currently regulated in the EU as a quarantine organism. Pathogenicity of the bacterium only occurs when a large proportion of xylem vessels in a plant are colonized thus many plants are asymptomatic carriers of X. fastidiosa, which can contribute to its spread. Early and sensitive detection could prevent the spreading and help effective pest control.
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM67050 (100 preps) | Cat. TM67010 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
Xylella fastidiosa Primer & Probe Mix | 280 μL | 280 μL |
Xylella fastidiosa Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |