Norgen Biotek offers comprehensive services for Next-generation sequencing (NGS) in an accredited state-of-the-art laboratory from sample isolation to sequencing. Norgen Biotek has extensive expertise in sample preparation, sequencing and analysis of all types of samples: cells, tissues, exosomes, as well as bodily fluids including plasma, serum, urine and saliva, as well as bacteria, viruses, and plant tissues. Our expert staff will assist in the initial consultation and sample handling to the final analysis of data presented in an easy to understand report. Your project will receive full attention by experts with a guaranteed fast turn-around time. Our services include: 1) Sample Isolation, 2) RNA Quantification and QC, 3) Library Preparation, 4) Library Quantification and QC, 5) Sequencing, and 6) Data Analysis, 7) Data Report.
Small RNA Sequencing from as little as 50 μL of Plasma. Norgen Biotek has developed an effective pipeline of small RNA sequencing from small volume of plasma or serum. RNA could be effectively and consistently recovered from as little as 50 μL of plasma using Norgen’s patented sample preparation technology (example shown here with Plasma/Serum RNA Purification Mini Kit, Cat# 55000). Panel A showed that the number of microRNA detected from 50 or 200 μL of Plasma was almost identical to that of 4 mL. Panel B is a Venn diagram showing that of the microRNAs identified, the majority are detected in all volume of plasma input from 50 μL to 4 mL. In fact, the scatter plots in Panel C showed the relative expression level of each microRNA detected was highly correlated between 50 or 200 μL of Plasma and 4 mL plasma.
Expert advice on sample handling, processing and experimental design for best data outcome
You can elect to simply submit isolated RNA or send the samples for RNA isolation; our team will guide you through what is required from different sample types
Isolated RNA will be quantified, assessed for quality and PCR amplification
Library preparation with industry standard kits, QC and quantification, Size selection
Filtering, aligning/mapping, annotating with statistical analysis
Comprehensive report detailing the analysis will be the final deliverable
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