Plant viruses cause the second highest losses to crop yields every year, surpassed only by fungi. A research team in Norway recently compared several in vitro virus elimination therapies on a variety of potato cultivars. Traditional meristem culture, cryotherapy, and a combination of chemotherapy and thermotherapy techniques were included in this study and assessed for their ability to eliminate potato-specific viruses from eight unique cultivars. Figure 1 provides an overview of the experimental procedure used in this study.
RNA isolation and quantification of miRNAs are gaining significance as promising methods in cancer biomarker discovery. Establishing standardized protocols for collection, sample storage conditions, and the use of exogenous and endogenous controls for normalization are all critical aspects to reliably quantify miRNA levels which can be further implemented in a clinical laboratory setting.
The National Institute of Health estimates that approximately 6 in 100 000 individuals develop brain or central nervous system-related cancers each year1. Current approaches in the diagnosis of brain cancer include CT scans, MRI and tissue biopsy. However, such techniques can be limited by tumour location and heterogeneity, and in the case of tissue biopsy, its highly invasive nature.