Intact Exosome Purification and Exosomal RNA Isolation
Intact Exosome Purification and Exosomal RNA Isolation
Exosomes: The Essentials
All-in-one systems for intact exosome purification and high-quality RNA isolation from the purified exosomes
- Purification and enrichment of intact exosomes from plasma/serum, urine and cell culture media followed by subsequent exosomal RNA isolation from the purified intact exosomes
- Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
- Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
- No precipitation reagents, overnight incubation, protease or coagulant treatments required
- No time-consuming ultracentrifugation, filtration or special syringes required
- Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
- Purified exosomes are compatible with functional studies.
- Purified exosomes are free from any protein-bound cell-free circulating RNA
- Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
- The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Norgen’s Exosome Purification and RNA Isolation Kits enable fast, flexible, robust, and convenient all-in-one systems for intact exosome purification from a wide range of cell-free liquid biopsies and cell-free cell culture media, in addition to the subsequent exosomal RNA isolation from the purified intact exosomes. Relying on Norgen’s proprietary resin technology, the intact exosomes are purified in 30 minutes and are free from any cell-free circulating protein-bound RNA which typically contaminate exosomes and impedes effective exosomal content analyses. The purified intact exosomes are amenable to labelling, counting, imaging, and in vivo / in vitro functional studies, as well as exosomal analyte isolations and analyses.
These kits also allow for the subsequent isolation of RNA from the previously purified intact extracellular vesicles (EVs), including exosomes. The purification is based on Norgen’s proprietary resin which isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. The high integrity purified RNA is isolated in a flexible elution volume ranging from 50 µL to 100 µL, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.