Cell Culture Media Exosome Purification and RNA Isolation Kits
All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media
For research use only and NOT intended for in vitro diagnostics.
Version Available Here.
Cell Culture Media Exosome Purification and RNA Isolation Kits
All-in-one system for the purification of exosomes and the subsequent isolation of RNA from cell culture media
Register today to receive an exclusive 15% off* on your first order.
Features and Benefits
- Purification and enrichment of intact cell culture media exosomes for functional studies
- Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
- Versatile cell culture media input volumes
- No phenol extractions, Proteinase K treatment, nor carrier RNA required
- No time-consuming ultracentrifugation, filtration nor special syringes required
- No precipitation reagents nor overnight incubation required
- Pure exosomes are purified and are free from any other RNA-binding proteins
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin
- The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Norgen's Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
For sample input volumes ranging from 5 mL to 10 mL.
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
For sample input volumes ranging from 10 mL to 20 mL.
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
For sample input volumes ranging from 20 mL to 35 mL.
Details
Supporting Data
Kit Specifications
|
|
Sample Type
|
Cell-Free Cell Culture Media
|
Sample Volume Range
|
10 mL to 20 mL
|
Size of RNA Purified |
All sizes, including miRNA
and small RNA (<200 nt) |
Elution Volume |
50-100 μL
|
Time to Complete 10 Purifications |
40-45 minutes
|
Average Yields* |
Variable depending on specimen
|
*Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60ºC if any salt precipitation is observed.
Component | Cat. 60700 (50 preps) | Cat. 60800 (25 preps) | Cat. 60900 (15 preps) |
---|---|---|---|
Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
ExoC Buffer | 1.5 mL | 1.5 mL | 1.5 mL |
ExoR Buffer | 12 mL | 12 mL | 12 mL |
Lysis Buffer A | 20 mL | 20 mL | 20 mL |
Lysis Additive B | 2 mL | 2 mL | 2 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Mini Filter Spin Column | 50 | 25 | 15 |
Mini Spin Columns | 50 | 25 | 15 |
Collection Tubes | 50 | 25 | 15 |
Elution tubes (1.7 mL) | 100 | 50 | 30 |
Product Insert | 1 | 1 | 1 |
Documentation
FAQs
Mini, Midi, Maxi
Citations
Title | Adipose-derived stem cell exosomes act as delivery vehicles of microRNAs in a dog model of chronic hepatitis |
Citation | Nanotheranostics 2024. |
Authors | Ilaria Zanolla1, Martina Trentini2, Elena Tiengo2, Federica Zanotti2, Tommaso Pusceddu2, Andrea Rubini3, Giuseppe Rubini4, Francesca Brugnoli4, Danilo Licastro5, Marco Debortoli5, Lucia Gemma Delogu6, Letizia Ferroni6, Luca Lovatti2, Barbara Zavan |
Title | Effects of microgravity on neural crest stem cells |
Citation | Frontiers in Neuroscience 2024. |
Authors | Yilin Han1† , Povilas Barasa2† , Lukas Zeger1†, Sara B. Salomonsson3‡ , Federica Zanotti4‡ , Marcel Egli5,6, Barbara Zavan4 , Martina Trentini4 , Gunnar Florin7 , Alf Vaerneus7, Håkan Aldskogius1 , Robert Fredriksson3 and Elena N. Kozlova1* |
Title | RNA-Binding Protein Motifs Predict microRNA Secretion and Cellular Retention in Hypothalamic and Other Cell Types |
Citation | Biomedicnes 2024. |
Authors | Wenyuan He 1 and Denise D. Belsham |