2X PCR Master Mix A ready-to-use solution optimized for robust amplification of high yield products Powered by Bioz 2X PCR Master Mix A ready-to-use solution optimized for robust amplification of high yield products USD$79.00 SKU 28007 Size 100 Reactions Quantity Overview Details Documentation Citations Overview Convenient ready-to-use solutionHigh sensitivity and yieldRobust amplification Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. Details Supporting Data Previous Figure 1. Successful Amplification of Different Sized Amplicons. Norgen’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. Norgen’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is Norgen’s PCR Sizer 100 bp DNA Ladder (Cat #11400) and Lane M2 is Norgen’s MidRanger 1kb DNA Ladder (Cat # 11700). Figure 2. Highly Stable after Multiple Freeze-Thaw Cycles The stability of Norgen’s 2X PCR Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. Norgen’s 2X PCR Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel. Next Click for expanded view Reagents Supplied - Cat # 28007 2X PCR Master Mix (1 Vial, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions Storage Conditions and Product Stability Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year. Documentation PROTOCOLS 28007 - Protocol (100 rxns) POSTERS 2X PCR Master Mix Sensitivity of DNA Extraction Methods From Different Bodily Fluids For Human Identification - Poster Citations Title Replicative Stress Coincides with Impaired Nuclear DNA Damage Response in COX4-1 Deficiency Journal Int. J. Mol. Sci.. 2022. Authors Liza Douiev , Chaya Miller, Guy Keller, Hadar Benyamini, Bassam Abu-Libdeh and Ann Saada Title Phenotypic and Phylogenetic Characterization of Micro-symbionts Nodulating Winged Bean (Psophocarpus tetragonolobus L. DC.) Landraces Journal West African Journal of Applied Ecology. 2022. Authors A . R. Oloyede, O. O. Oyelakin, S. O. Ogunesan Title Handbook On Cassava: Status Of Cassava Processing And Challenges In The Coastal, Eastern And Western Regions Of Kenya Journal Nova Science Publishers, Inc.. 2017 Authors C. M. Githunguri, M. Gatheru and S. M. Ragwa Title Molecular Detection of Virulence Genes and Antibiotic Resistance Patterns of Escherichia coli O157:H7 Isolated from Raw Beef Sold in Abeokuta, South-West Nigeria Journal Nigerian Journal of Biotechnology. 2016. Authors Oloyede, A. R., Afolabi, O. R., & Olalowo, O. S Title Identification Of Cuscuta Campestrisyuncker In Uae: Study Of Bar Code Loci-Rbcl, Matk And Trnh-Psbain The Uae And Egyptian Cultivars And In The Respective Host Plants Basil And Jute Journal International Journal of Research in Science. 2016. Authors Alsaadi, A., Saif, M., Yasser, M., Gaur, S., El-wakil, H., Maloukh, L., & Lakshmi, T. V. R Title Production of Lipases in Solid-State Fermentation by Aspergillus Niger F7-02 with Agricultural Residues Journal Journal of Microbiology, Biotechnology and Food Sciences. 2015. Authors Olayinka Quadri Adio, Sarafdeen Olateju Kareem, Michael Bamitale Osho, Adebukola Mobolaji Omemu Title Molecular Epidemiology of Escherichia Coli in HIV-Positive Individuals in South west Nigeria Journal International Archives of Medicine. 2015. Authors Lawrence Ehis Okoror, Duna Christina Fashina, Titilayo Silifat Jimoh, Ezekiel Ayo Oisagah Title Na+/H+ Exchanger Isoform 1 Induced Cardiomyocyte Hypertrophy Involves Activation of p90 Ribosomal S6 Kinase Journal PLOS ONE. 2015. Authors Maiy Jaballah, Iman A. Mohamed, Bayan Alemrayat, Fatima Al-Sulaiti, Mohamed Mlih, Fatima Mraiche Title TNF (-308) Polymorphism has no Influence on the Clinical Severity of Sickle Cell Anemia in Saudi Patients. Journal Journal of Applied Hematology. 2013. Authors Hamad Z, Aljedai A, Halwani R, Alsultan A. Title Microbial contamination of human milk purchased via the Internet Journal Pediatrics. 2013. Authors Keim SA, Hogan JS, McNamara KA, Gudimetla V, Dillon CE, Kwiek JJ, Geraghty SR. Title Characterization of unstable microsatellites in mice: No evidence for germline mutation induction following gamma-radiation exposure. Journal Environmental and Molecular Mutagenesis. 2012. Authors Beal MA, Glenn TC, Lance SL, Somers CM.
2X PCR Master Mix A ready-to-use solution optimized for robust amplification of high yield products Powered by Bioz 2X PCR Master Mix A ready-to-use solution optimized for robust amplification of high yield products USD$79.00 SKU 28007 Size 100 Reactions Quantity