Name: HIV Quantitative TaqMan RT-PCR Detection Kit
Brand: Norgen Biotek Corp.
SKU: TM33740
Price: 289.000000 USD
Availability: InStock

Overview

Contains ready-to-use Primer&Probe Mix with FAM probe, and 2X One-Step RT-PCR Master Mix for the quantification of HIV-1.
Comes with an RNA transcript from part of the HIV-1 genome quantified at 250,000 copies/µL.
Has a linear range (analytical measurement) from 2,000,000 to 20 copies in a 20 µL reaction.
This kit is designed for research use only and not for use in diagnostic procedures.

Norgen's HIV Quantitative TaqMan RT-PCR Detection Kit is a turn key solution which includes 2X One-Step RT-PCR master mix, HIV specific primer/probe mix, Nuclease-Free Water, and Quantified HIV positive control. Norgen's HIV specific primer and probe mix is designed to detect a 123 nt region of the HIV-1 genome using a taqman based assay utilizing the FAM fluorophore. Norgen's HIV positive control contains an RNA transcript from part of the HIV-1 genome at 250,000 copies/µL quantified by ddPCR to construct a dilution series for HIV RNA quantification.

This kit is designed for research use only and not for use in diagnostic procedures.

Norgen's HIV Quantitative TaqMan RT-PCR Detection Kit was developed and validated to be used with the following PCR instruments:

Qiagen Rotor-Gene Q
BioRad iCycler
BioRad CFX96 Touch
Applied Biosystems QuantStudioTM 5, 6 and 7Pro

Details

Supporting Data

Figure 1. The HIV quantified positive control dilution series generated from Norgen’s HIV Quantitative TaqMan RT-PCR Detection Kit (Cat. TM33740 ran on a BioRad C1000 CFX96 Real-Time PCR Machine.

Figure 2. An example standard curve generated from Norgen’s HIV Quantitative TaqMan RT-PCR Detection Kit (Cat. TM33740) ran on a BioRad C1000 CFX96 Real-Time PCR Machine.

Linear Range

he linear range (analytical measurement) of Norgen's HIV Quantitative TaqMan RT- PCR Detection Kit was determined by analysing a dilution series of Norgen's HIV quantification standard ranging from 2 x 10⁶ copies to 2 x 10¹ copies.
Each dilution has been tested in replicates (n = 4) using Norgen's HIV Quantitative TaqMan RT-PCR Detection Kit.

Storage Conditions and Product Stability

The HIV Quantitative TaqMan RT-PCR Detection Kit is shipped on dry ice.
All kit components should be stored at -20°C upon arrival
If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance.
Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
These reagents should remain stable for at least 1 year when stored at the specified conditions.

Component	Cat. TM33740 (48 samples)
2X One-Step RT-PCR Master Mix	3 x 350 μL
HIV Primer & Probe Mix	3 x 70 μL
HIV Quantified RNA Standard	100 μL
Nuclease-Free Water	1.25 mL
Product Insert	1

HIV Quantitative TaqMan RT-PCR Detection Kit