West Nile Virus (WNV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz West Nile Virus (WNV) TaqMan RT-PCR Detection Kits SMART Save USD$749.00 USD$599.20 SKU TM44250 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for WNV CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level. TaqMan PCR Kit Click to expand options WNV TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM44250 (100 preps) Cat. TM44210 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - WNV Primer & Probe Mix 280 μL 280 μL WNV Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM44250) West Nile Virus (WNV) TaqMan RT-PCR Kit - Protocol (100 rxns) SAFETY DATA SHEETS (TM44250) West Nile Virus (WNV) TaqMan RT-PCR Kit - Safety Data Sheet Related Products West Nile Virus (WNV) TaqMan RT-PCR Kit Dx Plasma/Serum RNA Purification Kit
West Nile Virus (WNV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz West Nile Virus (WNV) TaqMan RT-PCR Detection Kits SMART Save USD$749.00 USD$599.20 SKU TM44250 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity