Supporting Data

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MiniSizer 50 bp DNA Ladder (Cat# 11200) - 100 loads

Ladder Properties:
• Fourteen discrete bands, ranging from 50 bp to 650 bp
• Higher intensity bands at 200 bp and 400 bp for easy reference

 

Fragment	Size (bp)	Mass (ng)
1	650	61
2	600	46
3	550	36
4	500	30
5	450	35
6	400	85
7	350	27
8	300	25
9	250	16
10	200	54
11	150	33
12	100	21
13	50	17
14	25	14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

 

Documentation

PROTOCOLS

11200 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

SAFETY DATA SHEETS

11200 - Minisizer 50 BP DNA Ladder - SDS