CloneSizer 100 bp DNA Ladder Includes a 2686 bp (pUC19) reference band for easy clone identification For research use only and NOT intended for in vitro diagnostics. Powered by Bioz CloneSizer 100 bp DNA Ladder Includes a 2686 bp (pUC19) reference band for easy clone identification SKU 11600 Size 100 Loads Quantity Overview Details Documentation Citations Overview Ready-to-useQuantitativeHighly StablePreciseFourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp incrementsHigher intensity reference bands at 500 bp and 1000 bp2686 bp (pUC19) reference band for easy clone identification The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.Contents:1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS). Details Supporting Data Previous Figure 1: CloneSizer 100 bp DNA Ladder Next Click for expanded view CloneSizer 100 bp DNA Ladder (Cat# 11600) - 100 loads Ladder Properties: Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments Higher intensity reference bands at 500 bp and 1000 bp 2686 bp (pUC19) reference band for easy clone identification Fragment Size (bp) Mass (ng) 1 2686 72 2 2000 53 3 1500 41 4 1200 42 5 1000 56 6 900 30 7 800 29 8 700 25 9 600 25 10 500 52 11 400 19 12 300 20 13 200 19 14 100 17 Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well. Storage: Stable at room temperature. For longer term storage, -20°C is recommended. This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer. Documentation PROTOCOLS 11600 - Product Insert APPLICATION NOTES DNA Quantification by Gel Densitometry with Norgen DNA Ladders SAFETY DATA SHEETS CloneSizer 100 bp DNA Ladder - MSDS Citations Title PCR Based Screening of Fusarium sp. in some Organic Products Journal Bulletin UASVM Animal Science and Biotechnologies. 2015. Authors Oana M. BOLDURA, Petrica BOZAC, Sorina POPESCU Title Bacterial community shift in the coastal Gulf of Mexico salt-marsh sediment microcosm in vitro following exposure to the Mississippi Canyon Block 252 oil (MC252). Journal 3 Biotech. 2014. Authors Hyunmin Koo, Nazia Mojib, Jonathan P. Huang, Rona J. Donahoe, Asim K. Bej. Title UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2. Journal Extremophiles: Life Under Extreme Conditions. 2013. Authors Mojib N, Farhoomand A, Andersen DT, Bej AK. Title Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR. Journal Antonie Van Leeuwenhoek 97(3):275-87.. 2010. Authors Panicker G, Mojib N, Aislabie J, Bej AK. Title Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes. Journal Appl Environ Microbiol.. 2006. Authors Ward LN, Bej AK. Title Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays. Journal Appl Environ Microbiol.. 2005. Authors Panicker G, Call DR, Krug MJ, Bej AK. Title PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico. Journal Appl Environ Microbiol. 2003 Apr;69(4):2194-200. Authors Myers ML, Panicker G, Bej AK.
CloneSizer 100 bp DNA Ladder Includes a 2686 bp (pUC19) reference band for easy clone identification For research use only and NOT intended for in vitro diagnostics. Powered by Bioz CloneSizer 100 bp DNA Ladder Includes a 2686 bp (pUC19) reference band for easy clone identification SKU 11600 Size 100 Loads Quantity