Supporting Data

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Figure 1:  50 bp DNA Ladder

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50 bp DNA Ladder (Cat# 13525) - 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference

 

Fragment	Size (bp)	Mass (ng)
1	500	79
2	450	67
3	400	59
4	350	55
5	300	49
6	250	76
7	200	33
8	150	22
9	100	31
10	50	29

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

 

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Documentation

PROTOCOLS

13525 - Product Insert

APPLICATION NOTES

DNA Quantification by Gel Densitometry with Norgen DNA Ladders

SAFETY DATA SHEETS

13525 - 50 BP DNA Ladder - SDS