Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit
![Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit](/sites/default/files/styles/norproduct_imagekit/public/68300-web_2.jpg?itok=CAGZdfbH)
For research use only and NOT intended for in vitro diagnostics.
Aperçu du produit
Norgen’s cfc-DNA/cfc-RNA Advanced Fractionation Kit provides fast, reliable and simple procedures for the fractionation of the highest quality and quantity of all sizes of circulating DNA and circulating RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL into two separate elutions. Purification is based on using Norgen’s proprietary resin separation matrix. The kits are designed to isolate total cfc-nucleic acid from either fresh, preserved or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-RNA and cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum total cfc-nucleic acid is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Détails
Supporting Data
Figure 1. Representative Bioanalyzer RNA Pico Chip traces and High Sensitivity DNA Chip traces showing the quality/quantity of the RNA fraction and the DNA fraction purified simultaneously from the same 4 mL K2 EDTA plasma using both automated and manual isolation. A) Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit (DNA Fraction from Automated Isolation), B) Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit (DNA Fraction from Manual Isolation), C) Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit (RNA Fraction from Automated Isolation), D) Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit RNA Fraction from Manual Isolation). All purification methods showed the correct cf-RNA size on the Bioanalyzer trace.
Figure 2. RT-qPCR amplification of miR-21 and miR-16 from the RNA fraction purified from 4mL K2EDTA plasma using Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit, both manually and automated. There is no difference in the amplification of both miR-21 and miR-16 between the automated and the manual isolation.
Figure 3. Real-Time PCR amplification of ALU 247 and ALU 115 from the DNA fraction purified from 4mL K2EDTA plasma using Norgen’s Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit, both manually and automated. There is no difference in the amplification of both ALU 247 and ALU 115 between the automated and the manual isolation.
Kit Specifications
|
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Minimum Plasma/Serum Input
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1 mL |
Maximum Plasma/Serum Input
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6 mL
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Size of cfc-RNA Purified
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All sizes, including miRNA and small RNA (< 200 nt)
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Optimal size of purified cfc-DNA
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146 - 180 bp
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Elution Volume |
25-50 µL
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Time to Complete 10 Purifications |
45 minutes
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Average Yields* | Variable depending on specimen |
*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Component | Cat. 68300 (50 preps) |
---|---|
Binding Buffer A | 20 mL |
Proteinase K | 6.5 mL |
Slurry E | 12.5 mL |
Lysis Buffer A | 130 mL |
Wash Solution A | 2 x 38 mL |
Elution Buffer C | 2 x 8 mL |
Elution Buffer F | 1 x 6 mL 1 x 15 mL |
Elution Solution A | 6 mL |
Micro Spin Columns | 100 |
Mini Filter Spin Columns | 50 |
Collection Tubes | 150 |
Elution Tubes (1.7 mL) | 150 |
Product Insert | 1 |