Figure 1. Comparison of Total DNA Yield Isolated by Norgen's FFPE DNA Kit Dx and a Leading Competitor FFPE DNA Purification Kit. DNA was isolated from 10 mg of FFPE kidney blocks. DNA concentrations were measured using the NanoVue spectrophotometer (GE Healthcare). Norgen's kit was found to have a much higher DNA yield, compared to the competitor kit.
Figure 2. Comparison of DNA Quality Isolated by Norgen's FFPE DNA Kit Dx and a Leading Competitor FFPE DNA Purification Kit. DNA was isolated from 10 mg of FFPE kidney blocks. Quality was assessed using A260:A280 and A260:A230 ratios generated from the NanoVue spectrophotometer (GE Healthcare). While Norgen and the competitor kit were found to have similar A260:A280 ratios, Norgen was found to have a much higher A260:A230 ratio, indicating higher quality DNA.
Figure 3. Comparison of qPCR Performance from FFPE DNA Isolated by Norgen's FFPE DNA Kit Dx and a Leading Competitor FFPE DNA Purification Kit. Total DNA was isolated from 10 mg of FFPE kidney blocks, in triplicate. A qPCR reaction was performed using 500 ng of purified DNA in a 2 µL reaction, using Norgen's 2x qPCR Mastermix (Cat# 28007) spiked with SYBR Green (Bio-Rad). The reaction took place in the iCycler iQ™Real-Time PCR Detection System (Bio-Rad), using beta-actin primers. The graph depicts the average Ct value generated from DNA isolated from both kits. Norgen and the leading competitor were found to isolate DNA from FFPE tissues with the highest quality, performing similarly in a qPCR reaction.
Figure 4. Comparison of DNA molecular weight range isolated by Norgen's FFPE DNA Kit Dx and a Leading Competitor FFPE DNA Purification Kits. DNA was isolated from 10 mg of FFPE kidney blocks. Based on the intensity of the bands, Norgen's kit captured more total DNA. Moreover, the DNA isolated by Norgen's kit covered a larger MW size compared with that isolated by the competitor kit. Both very high molecular DNA (intact genomic DNA, black arrow) as well as small molecular weight DNA were recovered. M = Norgen's HighRanger Molecular Weight Ladder.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K and RNase should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 2 years in their unopened containers.
