FFPE DNA Purification Kit (Cat. 47400)
FFPE DNA Purification Kit
![FFPE DNA Purification Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/47400-Reflection-web.jpg?itok=LNMn-d2c" "FFPE DNA Purification Kit")
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FFPE DNA Purification Kit
For the rapid and efficient extraction and purification of DNA from FFPE samples
- Fast and easy processing using rapid and convenient spin-columns
- Isolate high quality and high yield DNA
- DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping
For research use only and NOT intended for in vitro diagnostics.
CE-IVDD marked diagnostic version available here
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(Cat. 47400) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity (gDNA)
|
10 μg
|
| Maximum Loading Volume Per Spin Column |
650 μL
|
| Size of DNA Purified |
All sizes > 80 bp
|
| Maximum Amount of Starting Material |
5 sections < 20 µm thick paraffin slices
25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
Supporting Data
| Component | Cat. 47400 (50 preps) |
|---|---|
| Digestion Buffer A | 25 mL |
| Buffer RL | 30 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 12 mg |
| RNase A | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Diversity of High-Risk and Low-Risk Human Papillomavirus Genotypes Oncogenated in ENT Tumors in Burkina Faso |
| Journal | Research Square. 2022. |
| Authors | Ali Kandé, Maïmouna Ilboudo, Djénéba Ouermi, Théodora Mahoukèdè Zohoncon, Esther Mah Alima Traoré, Ina Marie Angèle Traoré, Florencia W. Djigma, Yvette Marie Chantal Gyebre, Dorcas Obiri-Yeboah, Jacques Simpore |
| Title | Microbiome Evidence of Digestive Reflux and Inflammation in the Pancreatic Stump with Pancreatic Fistula After Pancreaticoduodenectomy |
| Journal | Research Square. 2022. |
| Authors | Tiegong Wang, Fengshan Li, Liang Tian, Yanfeng Xu, Lei Zhang, Xiulei Zhao, Deshuai Kong |
| Title | Identification of functional pathways and molecular signatures in neuroendocrine neoplasms by multi-omics analysis |
| Journal | Journal of Translational Medicine. 2022. |
| Authors | Viola Melone, Annamaria Salvati, Domenico Palumbo, Giorgio Giurato, Giovanni Nassa, Francesca Rizzo, Luigi Palo, Alessandro Giordano, Mariarosaria Incoronato, Mario Vitale, Caterina Mian, Immacolata Di Biase, Stefano Cristiano, Viviana Narciso, Monica Cantile, Annabella Di Mauro, Fabiana Tatangelo, Salvatore Tafuto, Roberta Modica, Claudia Pivonello, Marco Salvatore, Annamaria Colao, Alessandro Weisz & Roberta Tarallo |
| Title | Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin |
| Journal | Pakistan Journal of Biological Sciences. 2016. |
| Authors | T.M. Zohoncon, T.C. Ouedraogo, L.V.C. Brun, D. Obiri-Yeboah, W.F. Djigma, S. Kabibou, S. Ouattara, M. Gomina, A.T. Yonli, V.J.T.E. Bazie, C. Ouedraogo, O. Lompo, S.A. Akpona and J. Simpore |
FFPE DNA Purification Kit
This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.  The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping.  The protocol can be completed in as little as 1 hour.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity (gDNA)
|
10 μg
|
| Maximum Loading Volume Per Spin Column |
650 μL
|
| Size of DNA Purified |
All sizes > 80 bp
|
| Maximum Amount of Starting Material |
5 sections < 20 µm thick paraffin slices
25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47400 (50 preps) |
|---|---|
| Digestion Buffer A | 25 mL |
| Buffer RL | 30 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 12 mg |
| RNase A | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Diversity of High-Risk and Low-Risk Human Papillomavirus Genotypes Oncogenated in ENT Tumors in Burkina Faso |
| Journal | Research Square. 2022. |
| Authors | Ali Kandé, Maïmouna Ilboudo, Djénéba Ouermi, Théodora Mahoukèdè Zohoncon, Esther Mah Alima Traoré, Ina Marie Angèle Traoré, Florencia W. Djigma, Yvette Marie Chantal Gyebre, Dorcas Obiri-Yeboah, Jacques Simpore |
| Title | Microbiome Evidence of Digestive Reflux and Inflammation in the Pancreatic Stump with Pancreatic Fistula After Pancreaticoduodenectomy |
| Journal | Research Square. 2022. |
| Authors | Tiegong Wang, Fengshan Li, Liang Tian, Yanfeng Xu, Lei Zhang, Xiulei Zhao, Deshuai Kong |
| Title | Identification of functional pathways and molecular signatures in neuroendocrine neoplasms by multi-omics analysis |
| Journal | Journal of Translational Medicine. 2022. |
| Authors | Viola Melone, Annamaria Salvati, Domenico Palumbo, Giorgio Giurato, Giovanni Nassa, Francesca Rizzo, Luigi Palo, Alessandro Giordano, Mariarosaria Incoronato, Mario Vitale, Caterina Mian, Immacolata Di Biase, Stefano Cristiano, Viviana Narciso, Monica Cantile, Annabella Di Mauro, Fabiana Tatangelo, Salvatore Tafuto, Roberta Modica, Claudia Pivonello, Marco Salvatore, Annamaria Colao, Alessandro Weisz & Roberta Tarallo |
| Title | Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin |
| Journal | Pakistan Journal of Biological Sciences. 2016. |
| Authors | T.M. Zohoncon, T.C. Ouedraogo, L.V.C. Brun, D. Obiri-Yeboah, W.F. Djigma, S. Kabibou, S. Ouattara, M. Gomina, A.T. Yonli, V.J.T.E. Bazie, C. Ouedraogo, O. Lompo, S.A. Akpona and J. Simpore |
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