For the rapid and efficient extraction and purification of DNA from FFPE samples
|FFPE DNA Purification Kit||47400||50 preps|
FFPE DNA Purification Kit
This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping. The protocol can be completed in as little as 1 hour.
Maximum Column Binding Capacity (gDNA)
|Maximum Loading Volume Per Spin Column||
|Size of DNA Purified||
All sizes > 80 bp
|Maximum Amount of Starting Material||
5 sections < 20 µm thick paraffin slices
25 mg of unsectioned block
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K and RNase should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 2 years in their unopened containers.
|Title||Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin|
|Journal||Pakistan Journal of Biological Sciences. 2016.|
|Authors||T.M. Zohoncon, T.C. Ouedraogo, L.V.C. Brun, D. Obiri-Yeboah, W.F. Djigma, S. Kabibou, S. Ouattara, M. Gomina, A.T. Yonli, V.J.T.E. Bazie, C. Ouedraogo, O. Lompo, S.A. Akpona and J. Simpore|
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