FFPE DNA Purification Kit For the rapid and efficient extraction and purification of DNA from FFPE samples For research use only and NOT intended for in vitro diagnostics.CE-IVDR marked diagnostic version available here Powered by Bioz FFPE DNA Purification Kit For the rapid and efficient extraction and purification of DNA from FFPE samples Price USD$255.00 SKU 47400 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Fast and easy processing using rapid and convenient spin-columns Isolate high quality and high yield DNA DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping. The protocol can be completed in as little as 1 hour. Details Supporting Data Previous Figure 1. Comparison of Total DNA Yield Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit. DNA was isolated from 10 mg of FFPE kidney blocks. DNA concentrations were measured using the NanoVue spectrophotometer (GE Healthcare). Norgen's kit was found to have a much higher DNA yield, compared to the competitor kit. Figure 2. Comparison of DNA Quality Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit. DNA was isolated from 10 mg of FFPE kidney blocks. Quality was assessed using A260:A280 and A260:A230 ratios generated from the NanoVue spectrophotometer (GE Healthcare). While Norgen and the competitor kit were found to have similar A260:A280 ratios, Norgen was found to have a much higher A260:A230 ratio, indicating higher quality DNA. Figure 3. Comparison of qPCR Performance from FFPE DNA Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit. Total DNA was isolated from 10 mg of FFPE kidney blocks, in triplicate. A qPCR reaction was performed using 500 ng of purified DNA in a 2 µL reaction, using Norgen's 2x qPCR Mastermix (Cat# 28007) spiked with SYBR Green (Bio-Rad). The reaction took place in the iCycler iQ™Real-Time PCR Detection System (Bio-Rad), using beta-actin primers. The graph depicts the average Ct value generated from DNA isolated from both kits. Norgen and the leading competitor were found to isolate DNA from FFPE tissues with the highest quality, performing similarly in a qPCR reaction. Figure 4. Comparison of DNA molecular weight range isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kits. DNA was isolated from 10 mg of FFPE kidney blocks. Based on the intensity of the bands, Norgen's kit captured more total DNA. Moreover, the DNA isolated by Norgen's kit covered a larger MW size compared with that isolated by the competitor kit. Both very high molecular DNA (intact genomic DNA, black arrow) as well as small molecular weight DNA were recovered. M = Norgen's HighRanger Molecular Weight Ladder. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity (gDNA) 10 μg Maximum Loading Volume Per Spin Column 650 μL Size of DNA Purified All sizes > 80 bp Maximum Amount of Starting Material 5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment. ComponentCat. 47400 (50 preps)Digestion Buffer A25 mLBuffer RL30 mLWash Solution A38 mLElution Buffer B15 mLProteinase K12 mgRNase A1 tubegDNA Purification Micro Columns50Collection Tubes50Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS (47400) FFPE DNA Purification Kit - Protocol (50 preps) SAFETY DATA SHEETS (47400) FFPE DNA Purification Kit - Safety Data Sheet FLYERS Flyer Citations Title Diversity of High-Risk and Low-Risk Human Papillomavirus Genotypes Oncogenated in ENT Tumors in Burkina Faso Journal Research Square. 2022. Authors Ali Kandé, Maïmouna Ilboudo, Djénéba Ouermi, Théodora Mahoukèdè Zohoncon, Esther Mah Alima Traoré, Ina Marie Angèle Traoré, Florencia W. Djigma, Yvette Marie Chantal Gyebre, Dorcas Obiri-Yeboah, Jacques Simpore Title Microbiome Evidence of Digestive Reflux and Inflammation in the Pancreatic Stump with Pancreatic Fistula After Pancreaticoduodenectomy Journal Research Square. 2022. Authors Tiegong Wang, Fengshan Li, Liang Tian, Yanfeng Xu, Lei Zhang, Xiulei Zhao, Deshuai Kong Title Identification of functional pathways and molecular signatures in neuroendocrine neoplasms by multi-omics analysis Journal Journal of Translational Medicine. 2022. Authors Viola Melone, Annamaria Salvati, Domenico Palumbo, Giorgio Giurato, Giovanni Nassa, Francesca Rizzo, Luigi Palo, Alessandro Giordano, Mariarosaria Incoronato, Mario Vitale, Caterina Mian, Immacolata Di Biase, Stefano Cristiano, Viviana Narciso, Monica Cantile, Annabella Di Mauro, Fabiana Tatangelo, Salvatore Tafuto, Roberta Modica, Claudia Pivonello, Marco Salvatore, Annamaria Colao, Alessandro Weisz & Roberta Tarallo Title Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin Journal Pakistan Journal of Biological Sciences. 2016. Authors T.M. Zohoncon, T.C. Ouedraogo, L.V.C. Brun, D. Obiri-Yeboah, W.F. Djigma, S. Kabibou, S. Ouattara, M. Gomina, A.T. Yonli, V.J.T.E. Bazie, C. Ouedraogo, O. Lompo, S.A. Akpona and J. Simpore Related Products FFPE RNA/DNA Purification Plus Kit
FFPE DNA Purification Kit For the rapid and efficient extraction and purification of DNA from FFPE samples For research use only and NOT intended for in vitro diagnostics.CE-IVDR marked diagnostic version available here Powered by Bioz FFPE DNA Purification Kit For the rapid and efficient extraction and purification of DNA from FFPE samples Price USD$255.00 SKU 47400 Format Spin Column Size 50 Preps Quantity