Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.

• Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM72450 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL
Trichinella spiralis Primer & Probe Mix	280 μL
Trichinella spiralis Positive Control	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL
Product Insert	1

Documentation

PROTOCOLS

(TM72450) Trichinella spiralis TaqMan PCR Kit - Protocol (100 rxns)

SAFETY DATA SHEETS

TM72450 - Trichinella spiralis TaqMan PCR Detection Kit - SDS

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