Plant microRNA Purification Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plant microRNA Purification Kit SKU 54700 Size 25 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis. Détails Supporting Data Previous Figure 1. Fractionation of Large and Small RNA. Large RNA and small RNA were sequentially purified using Norgen's Plant miRNA Purification Kit from 50 mg of raspberry leaf tissue and the RNA profile was compared with the RNA isolated using Norgen's Plant/Fungi Total RNA Purification Kit (Cat. #25800). For visualization, 10 µL of RNA from 50 µL of RNA elution was loaded on 2% 1x MOPS agarose gel. Norgen's kit was able to isolate both the large and small RNA fractions, and the small RNA fraction does not contain any of the large RNA species. Figure 2. Efficiency of the concentration of miRNA using Norgen's Plant miRNA Purification Kit to detect three plant miRNAs. Small RNA was concentrated from 50 mg raspberry using Norgen's Plant miRNA Purification Kit. Three µL of the elution was directly used for cDNA generation and 3 µL of cDNA was used for the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). Blue: concentrated small RNA from Norgen's Plant miRNA Purification Kit, Red: Total RNA from Plant Total RNA Purification Kit (Cat. 25800), Circle: +RT, Cross: - RT. Figure 3. Isolation of Concentrated RNA that can be used in Sensitive Downstream Applications. Three different miRNA purification methods were compared for their ability to isolate and detect plant miRNA. miRNA was purified from grape using three miRNA purification kits according to the manufacture’s manual. Next, 3 µL of the elution was directly used for cDNA generation and 3 µL of cDNA was used for the CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) to detect the miRNA 159a. As it can be seen, only the miRNA purified using Norgen's kit (blue lines) was amplified, while the miRNA isolated using the other 2 kits failed to amplify (red and green lines). Both of the competitor’s kits rely on phenol to isolate the miRNA, and therefore residual phenol is likely interfering with the amplification. Norgen's kit isolates high yields of good quality miRNA without the use of phenol. Blue: Norgen Plant miRNA, Red: Competitor Q, Trizol: Green. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Minimum Elution Volume 20 μL Size of RNA Purified < 200 nt Amount of Starting Material: Plant Tissues 100 mg 5 x 106 cells Time to Complete 10 Purifications 25 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. ComponentCat. 54700 (25 preps)Lysis Buffer C30 mLWash Solution A18 mLElution Solution A6 mLFilter Columns25Large RNA Removal Columns25microRNA Enrichment Columns25Collection Tubes75Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS (54700) Plant microRNA Purification Kit - Protocol (25 prep) APPLICATION NOTES Plant microRNA Purification Kit Evaluation of Plant RNA Integrity Number Rin Generated Using An Agilent Bioanalyzer 2100 - Application Note Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation SAFETY DATA SHEETS (54700) Plant microRNA Purification Kit - Safety Data Sheet POSTERS The Importance of Sample Preparation For Plant Mirna Purification FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Related Products Plant RNA/DNA Purification Kit Plant/Fungi Total RNA Purification Kit
Plant microRNA Purification Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plant microRNA Purification Kit SKU 54700 Size 25 Preps Quantity Trouver un distributeur Contact