CleanAll DNA/RNA Clean-Up and Concentration Micro Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz CleanAll DNA/RNA Clean-Up and Concentration Micro Kit SKU 23800 Format Micro Size 50 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions. The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less. Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified. Détails Supporting Data Previous Figure 1. High Recoveries and Efficient Endotoxin Removal. High recoveries of DNA can be seen when using the CleanAll kit, and these high recoveries are not compromised even during endotoxin removal. In Panel A, 1 µg of a PCR product was cleaned using the CleanAll DNA/RNA Clean-Up and Concentration Micro Kit as well as Norgen's PCR Purification Kit. More than 0.9 µg of the DNA was recovered from both kits with a comparable percent recovery of over 90%. In Panel B, 10 µg of plasmid DNA preparation was cleaned from endotoxin by using the CleanAll DNA/RNA Kit. Endotoxin levels of the plasmid DNA sample were reduced from 0.75 EU/µg plasmid DNA to less 0.05 EU/µg plasmid DNA. Approximately 95% of the endotoxin units in the DNA sample were removed using the kit, therefore the kit allows for high recoveries coupled with exceptional cleanup. Figure 2: Clean-Up of RNA with High Recovery. Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit can be used to clean up various enzymatic reactions including DNase treatment. Lane 1 is the RNA input, while lanes 2-5 contain the RNA that has been cleaned using Norgen's CleanAll Kit. It can be seen that in all cases the recovery is high, and the purified RNA is intact and of a high quality. Next Click for expanded view Kit Specifications Column Binding Capacity 50 μg RNA 10 μg DNA Maximum Column Loading Volume 600 μL Minimum Elution Volume 20 μL Time to Complete 10 Purifications 20 minutes Size of RNA Purified All sizes, including small RNA (<200) Size of DNA Purified ≥ 100 bp* Average Recovery ≥90% for RNA ≥90% for DNA 100 bp to 10 kbp ≥75% for DNA ≥ 10 kbp * Applicable to smaller fragments or oligonucleotides with alternative protocol Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. ComponentCat. 23800 (50 preps)Binding Buffer H60 mLWash Solution K27 mLElution Buffer L15 mLMicro Spin Columns50Collection Tubes50Elution Tubes (1.7 mL)50Product Insert1 Documentation PROTOCOLS PI23800-11 CleanAll Kit Insert.pdf SAFETY DATA SHEETS (23800) CleanAll DNA/RNA Clean-up and Concentration Micro Kit - Safety Data Sheet POSTERS Effect of DNA Contaminants On Transfection Efficiency and Mutation Rate In Cho Cells - ASCB 2007 FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Related Products Total RNA Purification Kit Cytoplasmic & Nuclear RNA Purification Kit Leukocyte RNA Purification Kit microRNA Purification Kit
CleanAll DNA/RNA Clean-Up and Concentration Micro Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz CleanAll DNA/RNA Clean-Up and Concentration Micro Kit SKU 23800 Format Micro Size 50 Preps Quantity Trouver un distributeur Contact