Oligo Clean-Up and Concentration Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Oligo Clean-Up and Concentration Kit SKU 34100 Format Spin Column Size 50 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling.Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction. The kit provides a high quality product with up to 90% recovery. The purified oligos can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, in situ hybridization, and RNAi studies. Détails Supporting Data Previous Figure 1. Integrity of Purified DNA Oligonucleotides. Oligonucleotides of different sizes (15mer, 20mer, 42mer and 61mer) were purified using Norgen's Oligo Clean-Up and Concentration Kit, and the integrity of the oligonucleotides before and after cleaning were compared by running a 15% urea-PAGE gel. Equal volumes of the input (I) and cleaned (C) oligonucleotide were run, and as it can be seen the purified oligonucleotides were of a high quality and integrity. Please note that the kit can also clean a mixture of different oligo sizes, as shown on the far right two lanes. Figure 2. Integrity of Purified dsDNA. Samples of Norgen's MiniSizer DNA ladder (25bp-650bp) were purified using Norgen's Oligo Clean-Up and Concentration Kit, and the integrity of the dsDNA before and after cleaning was compared by running aliquots on a 2% agarose gel. Both the input volume and the elution volume were maintained at 50 µL, and 5 µL and 10 µL of the input and 5 µL and 10 µL of the elution were run on the gel for visual comparison. As it can be seen, the purified dsDNA was of a high quality and integrity. The overall recovery of the different ladder bands is 82% of the input. Figure 3. Integrity of Purified RNA. Samples of Norgen's 100b RNA ladder (100b-1000b) were purified using Norgen's Oligo Clean-Up and Concentration Kit, and the integrity of the RNA before and after cleaning was compared by running aliquots of the input and elution on a 1.4% formaldehyde-agarose gel. Both the input volume and elution were maintained at 50 µL, and 5 µL and 10 µL of the input and 5 µL and 10 µL of the elution were run on the gel for visual comparison. The kit provides efficient RNA recovery of all the ladder bands. The overall recovery of the different ladder bands is 84% of the input. Next Click for expanded view Kit Specifications Column Binding Capacity 10 μg for DNA or RNA Maximum Column Loading Volume 600 μL Size of DNA/RNA Purified > 10 nt, single stranded or double stranded Maximum Amount of Starting Material 10 μg of DNA or RNA Minimum Elution Volume 20 μL Time to Complete 10 Purifications 15 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. Component Cat. 34100 (50 preps) Buffer RL 30 mL Wash Solution A 20 mL Elution Solution A 6 mL Micro Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (34100) Oligo Clean-Up and Concentration Kit - Protocol (50 prep) SAFETY DATA SHEETS (34100) Oligo Clean-Up and Concentration Kit - Safety Data Sheet RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Related Products CleanAll DNA/RNA Clean-Up and Concentration Micro Kit PCR Purification Kit RNA Clean-Up and Concentration Kit
Oligo Clean-Up and Concentration Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Oligo Clean-Up and Concentration Kit SKU 34100 Format Spin Column Size 50 Preps Quantity Trouver un distributeur Contact