Name: Fecal DNA Collection & Preservation Mini Tubes
Brand: Norgen Biotek Corp.
SKU: 27650
Price: 119.000000 USD
Availability: InStock

Features and Benefits

Complete System:

Include a tube with liquid preservative and glass beads to immediately homogenize the hard fecal pellets using a vortex.
Sample Homogeneity:

Eliminates sample variability.
Inactivating:

Renders the sample non-infectious by preventing bacterial/fungal growth and inactivating viruses; eliminates odor.
Ambient Temperature Preservation:

Stable preservation for over 2 years for DNA.
Cost-Effective:

No cold shipping or storage required.
Robust Preservation:

Effective across various temperatures.

Applications:

DNA Isolation: Compatible with multiple isolation methods, including Norgen's Stool DNA Isolation Kit.

Microbiome profiling: A reliable and reproducible source of sample for microbial detection or metagenomics analysis via NGS, qPCR, ddPCR and other applications.

Metabolomics: Stool samples preserved with this preservative are compatible with SCFA and metabolomic analysis according to literature.

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Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV's from fecal samples

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Details

Supporting Data

Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods..

Figure 2. Collection of Mouse Fecal Samples in Norgen's Fecal DNA Collection and Preservation Mini Tubes. Approximately 30 mg (3-5 droppings) of mouse fecal samples were added to Norgen’s Fecal Collection and Preservation Mini Tubes and briefly vortexed. The preserved samples are ready to ship, or for isolation of DNA using compatible DNA isolation kits.

Figure 3. Resolution of DNA Isolated from 200 µL Preserved Mouse Fecal Samples using Norgen’s Stool DNA Isolation Kit (Cat. 27600). Briefly, 4-5 drops (<30 mg) of mouse fecal samples were collected using Norgen's Fecal DNA Collection and Preservation Mini Tubes (Cat. 27650). Stool DNA was isolated from the preserved samples using Norgen's Stool DNA Isolation Kit. For evaluation, 10 µL of each 50 µL elution were run on 1X TAE 1.2% agarose gel. Marker = Norgen’s HighRanger DNA Ladder.

Figure 4. Quality of Mouse Fecal DNA was Evaluated by 16s rDNA Amplification using Real-Time PCR. Two mouse fecal samples (red and blue) were collected and preserved using Norgen’s Fecal DNA Collection and Preservation Mini Tubes, and then 3 µL of DNA was used in 20 µL PCR reaction to detect 16s rDNA. No PCR inhibition was observed, indicating the high quality of DNA from mouse fecal samples for use in downstream applications.

Kit Specifications
Fecal Input	Maximum 30 mg (approximately 3-5 mouse droppings)
Stability of Fecal DNA at Room Temperature	2 years

Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Kit Components	Cat. 27650
Fecal DNA Collection & Preservation Mini Tubes (containing liquid preservative and beads)	12
Product Insert	1

FAQs

What does each fecal mini tube contain?

Each tube contains 600 μL of preservative and glass beads for easy homogenization.

What is the total volume of each fecal mini tube?

Each tube is a 2 mL screw cap tube. Some of the volume is already taken up by the preservative and the glass beads.

Is the stool preservative in mini tubes the same as that present in Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660)?

Yes, the fecal mini tubes share the same excellent preservative as supplied in Norgen's Stool Nucleic Acid Collection and Preservation Tubes.

Which Norgen kit is most compatible with fecal mini tubes?

The DNA can be isolated from samples collected, homogenized, and preserved in fecal mini tubes, using Norgen's Stool DNA isolation kit (Cat. 27600).

What is the maximum amount of fecal sample that can be added in each fecal mini tube?

Each fecal mini tube can take a maximum 30 mg sample (approximately 3-5 mouse droppings).

Citations

Title	Harnessing the Power of PCR Molecular Fingerprinting Methods and Next Generation Sequencing for Understanding Structure and Function in Microbial Communities
Citation	PCR: Methods and Protocols, Methods in Molecular Biology 2017.
Authors	Phadke, S., Salvador, A. F., Alves, J. I., Bretschger, O., Alves, M. M., & Pereira, M. A. (2017).

Fecal DNA Collection & Preservation Mini Tubes