Name: Fecal Swab Collection and Preservation System (Bulk Format)
Brand: Norgen Biotek Corp.
SKU: 45670-B
Price: 349.000000 USD
Availability: InStock

Features and Benefits

Complete System:

Include a tube with liquid preservative, and a swab with matching break point to easily deposit sample in the tube.
Sample Homogeneity:

Eliminates sample variability.
Inactivating:

Renders the sample non-infectious by preventing bacterial/fungal growth and inactivating viruses; eliminates odor.
Ambient Temperature Preservation:

Stable preservation for over 2 years for DNA and 7 days for RNA at room temperature.
Cost-Effective:

No cold shipping or storage required.
Robust Preservation:

Effective over a range of various temperatures.
Freezer Compatible:

The tubes can be stored at -80°C to extend the stabilization of nucleic acids for longer periods.

Applications:

Optimized for difficult samples: This option is ideal for collecting less solid stool samples, making it particularly suitable for infants and patients with irritable bowel syndrome (IBS).

DNA and RNA Isolation: Compatible with multiple isolation methods, including Norgen's Microbiome DNA Isolation Kit , Stool total RNA and total nucleic acid isolation kits.

Microbiome profiling: A reliable and reproducible source of sample for microbial detection or metagenomics analysis via NGS, qPCR, ddPCR and other applications.

Metabolomics: Stool samples preserved with this preservative are compatible with SCFA and metabolomic analysis according to literature.

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Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV's from fecal samples

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Details

Supporting Data

Figure 1. Norgen's Best-In-Class Preservation. Summary of community shifts in response to stabilizing solutions over a 14-day storage period. Bray-Curtis distance towards the reference for each patient grouped by stabilizing solution. Median and 5th-to-95th percentile ranges are shown for both interaliquot and interpatient variability (adapted from Plauzolles et al, 2022). Norgen is the most similar to frozen stool samples, over 11 other sample collection and storage methods..

Figure 2. Integrity of Total Stool DNA and RNA Isolated from Preserved Stool Samples. DNA and RNA were isolated from 200 µL of preserved stool from 4 different donors (lanes A to D) stored at room temperature at the indicated time points (DNA at 27 months and RNA at 7 days) using Norgen's Stool Nucleic Acid Isolation Kit (Cat# 45600). Next, 10 µL from the 75 µL eluted nucleic acid was loaded onto a gel for visual analysis. High integrity stool DNA was isolated from preserved stool samples that were stored at room temperature for 27 months, and high integrity RNA was isolated from stool samples stored for 7 days at ambient temperature. Lane M is Norgen's HighRanger 1 kb DNA Ladder (Cat. 11900).

Figure 3. Monitoring the DNA stability of Adenovirus (Ad5subE1) Spiked into Preserved Stool Samples using TaqMan Real-Time PCR Assay. Preserved stool samples were spiked with 6 x 10⁹ pfμ/mL of Adenovirus, and 200 µL samples were removed at the indicated time points. The stool DNA was then isolated using Norgen's Stool DNA Isolation Kit (Cat. 27600). Next, 2 µL of DNA template from each 50 µL of elution was used in a 20 µL PCR reaction to detect the Adenovirus DNA. The stool preservative was shown to stabilize Adenovirus DNA up to 21 weeks at ambient temperature, indicating that Norgen's Fecal Swab Collection and Preservation System provides a safe and convenient sample transportation system.

Figure 4. Hierarchical Clustering of Stool Samples at Phylum Level Under Different Storage Conditions. The microbial composition from the stool samples preserved in Norgen’s Stool Nucleic Acid Preservative did not differ significantly to that of controls (fresh stool from Day 0 at RT) over a 2 day period, while non-preserved stool samples showed a greater difference in the dendrogram from the original stool profile.

Figure 5. Difference In Top 5 Abundant Microorganisms at Phylum Level Compared to Fresh Stool (Day 0). Abundance of Firmicutes, Bacteroidetes and proteobacteria from the preserved stool sample was higher than non-preserved (NP) compared to the fresh stool after 2 days storage at RT, indicating that the microorganism profiles from the preserved stool are much closer to the fresh stool sample (Day 0).

Figure 6. Relative Abundance of Bacterial Genera Between Preserved And Non-Preserved Stool Samples Stored at RT For 2 Days. Stool samples preserved using Norgen’s Stool Nucleic Acid Preservative showed a closed relativeness to the fresh stool sample (Day 0) compared to the non-preserved sample.

Figure 7. Correlation between duplicate bacterial metagenomic reads using (A) Fresh stool (day 0) and preserved stool at RT for 2 days and (B) Fresh stool (day 0) and non-preserved stool at RT for 2 days. A greater R2 was observed for Fresh stool (day 0) and preserved stool, suggestive of higher consistency between fresh stool sample and preserved stool sample.

Kit Specifications
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA Up to 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

The Fecal Swab Collection and Preservation System (Bulk Format) can be stored at room temperature for up to 2 years without any reduction in kit performance. The expiry date for the swabs is written on the swab wrapper.

Kit Components	Cat. 45670-B
Individually Wrapped Swabs	50
Fecal Swab Collection and Preservation Tube	50
Product Insert	1

Documentation

PROTOCOLS

(45670-B) Fecal Swab Collection and Preservation System - Protocol (Bulk Format)

SUPPLEMENTARY PROTOCOLS

Donor Procedure

Citations

Title	Analysis of Intestinal Microbiota and Metabolic Pathways before and after a 2-Month-Long Hydrolyzed Fish and Rice Starch Hypoallergenic Diet Trial in Pruritic Dogs
Citation	Veterinary Sciences 2023.
Authors	Chiara Noli, Antonella Varina, Caterina Barbieri, Alessandra Pirola and Daniela Olivero

Title	Identifying Microbiome Dynamics in Pediatric IBD: More than a Family Matter
Citation	Biomedicines 2023.
Authors	Nikolas Dovrolis, Anastasia Moschoviti, Smaragdi Fessatou ,George Karamanolis, George Kolios and Maria Gazouli

Fecal Swab Collection and Preservation System