Microbiome DNA Isolation Kit (Cat. 64100)
Microbiome DNA Isolation Kit
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Microbiome DNA Isolation Kit
Isolate total DNA from a variety of Microbiome samples
- Universal method to isolate and detect microorganisms and host cells simultaneously
- For use with samples collected using a swab - fresh, frozen or preserved in Norgen's Swab Collection and DNA Preservation System or Norgen's Fecal Swab Collection and Preservation System
- Rapid and convenient spin-column format
- Remove all PCR inhibitors from DNA samples
- Isolate high quality total DNA for PCR and NGS applications (Microbiome/Metagenomic sequencing)
- Optimized to isolate from the full preservative volume of the Swab Collection and DNA Preservation System for maximum yield
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 64100) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
| Maximum sample input |
1 mL of preserved swab sample* or up to 0.5 mL preserved samples**
|
| Swab samples tested |
Fecal, saliva, buccal, food, nasal, blood, surface, skin
|
| Maximum Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
| Time to Complete 10 Purifications |
30 minutes
|
* Collected using Norgen’s Swab Collection and DNA Preservation Kit (Cat. 45690)
**Please see the table in Section B of the Product Insert - Samples Collected using Norgen's Preservation Devices
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment.
| Component | Cat. 64100 (50 preps) |
|---|---|
| Lysis Buffer E | 55 mL |
| Lysis Additive A | 6 mL |
| Binding Buffer I | 7 mL |
| Binding Buffer B | 30 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus |
| Journal | Scientific Reports. 2022. |
| Authors | Yonathan Tilahun, Jessica Quijada Pinango, Felicia Johnson, Charles Lett, Kayla Smith, Terry Gipson, Malcolm McCallum, Peter Hoyt, Andrew Tritt, Archana Yadav, Mostafa Elshahed & Zaisen Wang |
| Title | First line of defence: Skin microbiota may protect anurans from infective larval lungworms |
| Journal | International Journal for Parasitology: Parasites and Wildlife. 2021. |
| Authors | Keit Christian, Richard Shine, Kimberley A. Day, Mirjam Kaestli, Karen Gibb, Catherine M. Shilton, Gregory P. Brown |
Microbiome DNA Isolation Kit
Norgen's Microbiome DNA Isolation Kit provides a convenient and rapid method to isolate total DNA from a variety of Microbiome samples collected using a swab. This kit can be used for fresh (dry transport) swab or any swab preserved using Norgen's Swab Collection and DNA Preservation Kit or any other of Norgen's Preservation Systems. This universal protocol conveniently allows for the isolation of total genomic DNA from both host and microbial cells found in the swab sample simultaneously. The kit removes all traces of PCR impurities using the provided combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with downstream PCR or metagenomics using NGS.
Kit Specifications
|
Kit Specifications
|
|
| Maximum sample input |
1 mL of preserved swab sample* or up to 0.5 mL preserved samples**
|
| Swab samples tested |
Fecal, saliva, buccal, food, nasal, blood, surface, skin
|
| Maximum Column Binding Capacity |
50 μg
|
| Maximum Column Loading Volume |
650 μL
|
| Time to Complete 10 Purifications |
30 minutes
|
* Collected using Norgen’s Swab Collection and DNA Preservation Kit (Cat. 45690)
**Please see the table in Section B of the Product Insert - Samples Collected using Norgen's Preservation Devices
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment.
| Component | Cat. 64100 (50 preps) |
|---|---|
| Lysis Buffer E | 55 mL |
| Lysis Additive A | 6 mL |
| Binding Buffer I | 7 mL |
| Binding Buffer B | 30 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus |
| Journal | Scientific Reports. 2022. |
| Authors | Yonathan Tilahun, Jessica Quijada Pinango, Felicia Johnson, Charles Lett, Kayla Smith, Terry Gipson, Malcolm McCallum, Peter Hoyt, Andrew Tritt, Archana Yadav, Mostafa Elshahed & Zaisen Wang |
| Title | First line of defence: Skin microbiota may protect anurans from infective larval lungworms |
| Journal | International Journal for Parasitology: Parasites and Wildlife. 2021. |
| Authors | Keit Christian, Richard Shine, Kimberley A. Day, Mirjam Kaestli, Karen Gibb, Catherine M. Shilton, Gregory P. Brown |
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