Microbiome DNA Isolation Kit Isolate total DNA from a variety of Microbiome samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Microbiome DNA Isolation Kit Isolate total DNA from a variety of Microbiome samples Price USD$299.00 SKU 64100 Format Spin Column Size 50 Preps Quantity Overview Details Documentation Citations Overview Universal method to isolate and detect microorganisms and host cells simultaneously For use with samples collected using a swab - fresh, frozen or preserved in Norgen's Swab Collection and DNA Preservation System or Norgen's Fecal Swab Collection and Preservation System Rapid and convenient spin-column format Remove all PCR inhibitors from DNA samples Isolate high quality total DNA for PCR and NGS applications (Microbiome/Metagenomic sequencing) Optimized to isolate from the full preservative volume of the Swab Collection and DNA Preservation System for maximum yield Norgen's Microbiome DNA Isolation Kit provides a convenient and rapid method to isolate total DNA from a variety of Microbiome samples collected using a swab. This kit can be used for fresh (dry transport) swab or any swab preserved using Norgen's Swab Collection and DNA Preservation Kit or any other of Norgen's Preservation Systems. This universal protocol conveniently allows for the isolation of total genomic DNA from both host and microbial cells found in the swab sample simultaneously. The kit removes all traces of PCR impurities using the provided combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with downstream PCR or metagenomics using NGS. Details Kit Specifications Maximum sample input 1 mL of preserved swab sample* or up to 0.5 mL preserved samples** Swab samples tested Fecal, saliva, buccal, food, nasal, blood, surface, skin Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Time to Complete 10 Purifications 30 minutes * Collected using Norgen’s Swab Collection and DNA Preservation Kit (Cat. 45690) **Please see the table in Section B of the Product Insert - Samples Collected using Norgen's Preservation Devices Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. Component Cat. 64100 (50 preps) Lysis Buffer E 55 mL Lysis Additive A 6 mL Binding Buffer I 7 mL Binding Buffer B 30 mL Wash Solution A 18 mL Elution Buffer B 8 mL Mini Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS PI64100-6 Microbiome DNA Isolation Kit Insert.pdf SAFETY DATA SHEETS 64100_SDS.pdf Citations Powered by Bioz See more details on Bioz Related Products Milk Bacterial DNA Isolation Kit Saliva DNA Isolation Kit Saliva DNA Collection and Preservation Devices
Microbiome DNA Isolation Kit Isolate total DNA from a variety of Microbiome samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Microbiome DNA Isolation Kit Isolate total DNA from a variety of Microbiome samples Price USD$299.00 SKU 64100 Format Spin Column Size 50 Preps Quantity