Microbiome DNA Isolation Kit (Cat. 64100)

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DNA Purification Kits
Total Genomic DNA

Microbiome DNA Isolation Kit

Cat. 64100
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Microbiome DNA Isolation Kit

Cat. 64100

Isolate total DNA from a variety of Microbiome samples

For research use only and NOT intended for in vitro diagnostics.

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Microbiome DNA Isolation Kit
(Cat. 64100) 50 preps

Kit Size

Microbiome DNA Isolation Kit

Norgen's Microbiome DNA Isolation Kit provides a convenient and rapid method to isolate total DNA from a variety of Microbiome samples collected using a swab. This kit can be used for fresh (dry transport) swab or any swab preserved using Norgen's Swab Collection and DNA Preservation Kit or any other of Norgen's Preservation Systems. This universal protocol conveniently allows for the isolation of total genomic DNA from both host and microbial cells found in the swab sample simultaneously. The kit removes all traces of PCR impurities using the provided combination of chemical and physical homogenization and lysis.  A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with downstream PCR or metagenomics using NGS.

Kit Specifications

You have selected: Cat. 64100
Kit Specifications
Maximum sample input
1 mL of preserved swab sample* or up to 0.5 mL preserved samples**
Swab samples tested
Fecal, saliva, buccal, food, nasal, blood, surface, skin
Maximum Column Binding Capacity
50 Î¼g
Maximum Column Loading Volume
650 Î¼L
Time to Complete 10 Purifications
30 minutes

* Collected using Norgen’s Swab Collection and DNA Preservation Kit (Cat. 45690)
**Please see the table in Section B of the Product Insert - Samples Collected using Norgen's Preservation Devices

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment.


Component Cat. 64100 (50 preps)
Lysis Buffer E 55 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
Binding Buffer B 30 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Mini Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Protocols: 
64100 - Protocol (50 preps)
Safety Data Sheets: 
64100 - Safety Data Sheet

Title Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus
Journal Scientific Reports. 2022.
Authors Yonathan Tilahun, Jessica Quijada Pinango, Felicia Johnson, Charles Lett, Kayla Smith, Terry Gipson, Malcolm McCallum, Peter Hoyt, Andrew Tritt, Archana Yadav, Mostafa Elshahed & Zaisen Wang
Title First line of defence: Skin microbiota may protect anurans from infective larval lungworms
Journal International Journal for Parasitology: Parasites and Wildlife. 2021.
Authors Keit Christian, Richard Shine, Kimberley A. Day, Mirjam Kaestli, Karen Gibb, Catherine M. Shilton, Gregory P. Brown

Microbiome DNA Isolation Kit

Norgen's Microbiome DNA Isolation Kit provides a convenient and rapid method to isolate total DNA from a variety of Microbiome samples collected using a swab. This kit can be used for fresh (dry transport) swab or any swab preserved using Norgen's Swab Collection and DNA Preservation Kit or any other of Norgen's Preservation Systems. This universal protocol conveniently allows for the isolation of total genomic DNA from both host and microbial cells found in the swab sample simultaneously. The kit removes all traces of PCR impurities using the provided combination of chemical and physical homogenization and lysis.  A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with downstream PCR or metagenomics using NGS.

Kit Specifications

You have selected: Cat. 64100
Kit Specifications
Maximum sample input
1 mL of preserved swab sample* or up to 0.5 mL preserved samples**
Swab samples tested
Fecal, saliva, buccal, food, nasal, blood, surface, skin
Maximum Column Binding Capacity
50 Î¼g
Maximum Column Loading Volume
650 Î¼L
Time to Complete 10 Purifications
30 minutes

* Collected using Norgen’s Swab Collection and DNA Preservation Kit (Cat. 45690)
**Please see the table in Section B of the Product Insert - Samples Collected using Norgen's Preservation Devices

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment.

Component Cat. 64100 (50 preps)
Lysis Buffer E 55 mL
Lysis Additive A 6 mL
Binding Buffer I 7 mL
Binding Buffer B 30 mL
Wash Solution A 18 mL
Elution Buffer B 8 mL
Mini Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Protocols: 
64100 - Protocol (50 preps)
Safety Data Sheets: 
64100 - Safety Data Sheet

Title Transcript and blood-microbiome analysis towards a blood diagnostic tool for goats affected by Haemonchus contortus
Journal Scientific Reports. 2022.
Authors Yonathan Tilahun, Jessica Quijada Pinango, Felicia Johnson, Charles Lett, Kayla Smith, Terry Gipson, Malcolm McCallum, Peter Hoyt, Andrew Tritt, Archana Yadav, Mostafa Elshahed & Zaisen Wang
Title First line of defence: Skin microbiota may protect anurans from infective larval lungworms
Journal International Journal for Parasitology: Parasites and Wildlife. 2021.
Authors Keit Christian, Richard Shine, Kimberley A. Day, Mirjam Kaestli, Karen Gibb, Catherine M. Shilton, Gregory P. Brown

 

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