Name: Total Nucleic Acid Preservation Tubes Dx
Brand: Norgen Biotek Corp.
SKU: Dx69200
Price: 349.000000 USD
Availability: InStock

Features and Benefits

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro diagnostic workflows
No need to immediately process samples
Total DNA, including viral DNA, preserved at room temperature over 4 months
Total RNA, including viral RNA, preserved at room temperature over 2 months
Inactivate microorganisms including bacteria, fungi, yeast and viruses
Inactivates SARS-CoV-2 virus - Explore the Application Note
Ship swab samples at room temperature safely
Compatible with most DNA and RNA isolation methods

Norgen’s Total Nucleic Acid Preservation Tubes Dx are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The Preservative prevents the growth of Gram-negative and Gram- positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months. This kit is ideal for collecting and preserving nasal and oral swab samples for in vitro diagnostic use for medical purposes. This kit can be used to preserve viral RNA, including SARs-CoV-2 RNA for detection of COVID-19.

Norgen’s Total Nucleic Acid Preservation Tubes Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s Total Nucleic Acid Preservation Tubes Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate these devices in conjunction with a downstream in vitro diagnostic assay.

Details

Supporting Data

Figure 1. Stability of RNA and DNA stored in Norgen's Total Nucleic Acid Preservative. GAPDH (for DNA) and β-actin (for RNA) were monitored over an 18 week period for DNA and 60 days for RNA from swab samples stored at ambient temperature in Norgen’s Total Nucleic Acid Preservation Tubes. RNA was isolated from 250 µL of preserved swab samples on day 0, day 4, day 6, day 10, day 16, day 30 and day 60 using Norgen’s Total RNA Isolation Kit (Cat.# 17200). DNA was isolated from 250 µL of preserved swab samples on day 0 and 18 weeks using Norgen’s Saliva DNA Isolation Kit (Cat# Dx45400). Next, 4 µL of elution was used in a 20 µL real-time PCR reaction using CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). RNA can be stored in Norgen’s Total Nucleic Acid Preservative for up to 60 days and DNA can be stored for up to 18 weeks at room temperature with no change in quality and quantity of RNA and DNA.

Figure 2. Stability of RNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the RNase Protein (RP) gene from 5 oropharyngeal swab samples and 5 nasopharyngeal swab samples purified using Norgen’s Total RNA Purification Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan One-Step RT-PCR.

Figure 3. Stability of DNA from different swab sample types stored in Norgen's Total Nucleic Acid Preservative. Amplification of the GAPDH gene from 5 oropharyngeal swab samples and from 5 nasopharyngeal swab samples purified using Norgen's Saliva DNA Isolation Kit were successfully validated from both swab sample types with high efficiency (<40 Ct.) by TaqMan PCR.

Figure 4. Inhibitory Effects of Norgen’s Total Nucleic Acid Preservative on the Growth of Bacteria, Yeast and Viruses. The effect of Norgen’s Total Nucleic Acid Preservative on the growth of microorganisms clearly demonstrated that Norgen’s preservative inhibits the growth of virus, bacteria and yeast, thereby resulting in non-infectious samples that can be handled and shipped safely.

Figure 5. Downstream Application of Preserved Swab RNA & DNA. The application of the RNA or DNA purified from preserved oropharyngeal swab samples was evaluated to detect RNase P (house keeping gene) and 16S rRNA (bacterial DNA) from swab samples collected from 60 donors and the preserved at room temperature overnight. Each individual swab sample contains different levels of cells, contributing to the variable Ct of each gene detection, but all passed the acceptance criteria for valid RT-PCR and PCR (< 35Ct, red line) in the 20 µL reaction, indicating that preserved swab RNA and DNA stored in Norgen’s Total Nucleic Acid Preservative is of high quality for downstream applications, such as real-time RT-PCR and PCR.

Kit Specifications
Stability of DNA at Room Temperature	4 months
Stability of RNA at Room Temperature	2 months

Shelf Life and Handling

The tubes should be kept tightly sealed and stored at room temperature for up to 1 year without any reduction in performance (The collection due date is written on the device label and the kit box label).

Once collected, RNA is stable for up to 2 months and DNA is stable for up to 4 months when the tubes are kept tightly sealed and stored at room temperature.

Kit Components	Cat. Dx69200
Total Nucleic Acid Preservation Tubes Dx	50
Product Insert	1

Documentation

Citations

Title	Prevalence of SARS-CoV-2 infection among obstetric patients in Ottawa, Canada: a descriptive study
Journal	CMAJ Open .2022.
Authors	Fakhraei, Romina, Erica Erwin, Kameela M. Alibhai, Malia SQ Murphy, Alysha LJ Dingwall-Harvey, Ruth Rennicks White, Sheryll Dimanlig-Cruz et al

Total Nucleic Acid Preservation Tubes Dx