At Norgen Biotek Corp. we are doing our part in response to the global coronavirus SARS-CoV-2 outbreak. | Find out how

 

Purchasing Information


  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification
  • Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
  • This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control 

A ready-to-use solution optimized for robust RT-PCR amplification

ItemsCat. #Size
2X One-Step RT-PCR Master Mix 28113 100 reactions
2X One-Step RT-PCR Master Mix 28114 200 reactions
2X One-Step RT-PCR Master Mix 28115 500 reactions

  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification
  • Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
  • This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control 


2X One-Step RT-PCR Master Mix

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

Reagents supplied – Cat # 28113

  • 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents supplied – Cat # 28114

  • 2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions

Reagents supplied – Cat # 28115

  • 2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.


ItemsCat. #Size
2X One-Step RT-PCR Master Mix 28113 100 reactions
2X One-Step RT-PCR Master Mix 28114 200 reactions
2X One-Step RT-PCR Master Mix 28115 500 reactions

  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification
  • Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
  • This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control 


2X One-Step RT-PCR Master Mix

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

Reagents supplied – Cat # 28113

  • 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions

Reagents supplied – Cat # 28114

  • 2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions

Reagents supplied – Cat # 28115

  • 2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

 

Related Products