2X One-Step RT-PCR Master Mix

Cat. 28113, 28114, 28115
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2X One-Step RT-PCR Master Mix

Cat. 28113, 28114, 28115

A ready-to-use solution optimized for robust RT-PCR amplification

  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification
  • Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
  • This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control 

For research use only and NOT intended for in vitro diagnostics.

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2X One-Step RT-PCR Master Mix
(Cat. 28113)
100 reactions
2X One-Step RT-PCR Master Mix
(Cat. 28114)
200 reactions
2X One-Step RT-PCR Master Mix
(Cat. 28115)
500 reactions

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification
  • Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
  • This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control 

2X One-Step RT-PCR Master Mix

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

Kit Specifications

You have selected: Cat. 28113

Reagents supplied – Cat # 28113

  • 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions


Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

You have selected: Cat. 28114

Reagents supplied – Cat # 28114

  • 2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions


Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

You have selected: Cat. 28115

Reagents supplied – Cat # 28115

  • 2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions


Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Supporting Data

Click for expanded view

Title Translational feasibility and efficacy of nasal photodynamic disinfection of SARS-CoV-2
Journal Scientific Reports. 2022.
Authors Layla Pires, Brian C. Wilson, Rod Bremner, Amanda Lang, Jeremie Larouche, Ryan McDonald, Joel D. Pearson, Daniel Trcka, Jeff Wrana, James Wu & Cari M. Whyne

2X One-Step RT-PCR Master Mix

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.

Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:

Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 28113

Reagents supplied – Cat # 28113

  • 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions


Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

You have selected: Cat. 28114

Reagents supplied – Cat # 28114

  • 2X One-Step RT-PCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions


Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

You have selected: Cat. 28115

Reagents supplied – Cat # 28115

  • 2X One-Step RT-PCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions


Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Title Translational feasibility and efficacy of nasal photodynamic disinfection of SARS-CoV-2
Journal Scientific Reports. 2022.
Authors Layla Pires, Brian C. Wilson, Rod Bremner, Amanda Lang, Jeremie Larouche, Ryan McDonald, Joel D. Pearson, Daniel Trcka, Jeff Wrana, James Wu & Cari M. Whyne